FISH Tag™ DNA Kits with Alexa Fluor™ Dyes
FISH Tag™ DNA Kits with Alexa Fluor™ Dyes
Citas y referencias (1)
Invitrogen™

FISH Tag™ DNA Kits with Alexa Fluor™ Dyes

FISH Tag DNA kits provide a complete solution for fluorescence in situ hybridization (FISH) applications. Choose from kits using Alexa Fluor 488, Alexa Fluor 555, Alexa Fluor 594, Alexa Fluor 647, or multicolor dyes for probe synthesis, purification, and labeling of specimens for imaging.
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Número de catálogoEtiqueta o tinteColor
F32948Alexa Fluor™ 555Naranja
F32947Alexa Fluor™ 488Verde
F32949Alexa Fluor™ 594Rojo
F32951Alexa Fluor™ 488, Alexa Fluor™ 555, Alexa Fluor™ 594, Alexa Fluor™ 647, Alexa Fluor 555, Alexa Fluor 594, Alexa Fluor 647Multicolor: verde, naranja, rojo, rojo lejano
Número de catálogo F32948
Precio (MXN)
-
Etiqueta o tinte:
Alexa Fluor™ 555
Color:
Naranja

Choose from three, single-color FISH Tag DNA kits with Alexa Fluor dye options (green, orange, or red). The FISH DNA multicolor kit contains four spectrally distinct dyes (green, orange, red, and deep-red) to allow simultaneous localization of multiple sequence-specific DNA targets in chromosomal spreads and in situ analysis of mitochondrial DNA localization and content. All kits include 16 reagents and come with a comprehensive, detailed protocol to help ensure your success.

FISH Tag™ DNA Kits provide a complete workflow solution for fluorescence in situ hybridization (FISH) applications. Each kit provides all of the reagents needed for probe synthesis, labeling, and purification, as well as for imaging the labeled specimen. Choose from FISH Tag™ DNA Kits in three Alexa Fluor™ colors or get the FISH Tag™ DNA Multicolor Kit (Cat. No. F32951), which contains four spectrally distinct dyes to allow simultaneous localization of multiple sequence-specific DNA targets in chromosomal spreads and for in situ analysis of mitochondrial DNA localization and content. All kits include 16 reagents and come with a comprehensive, detailed protocol to help ensure your success.

FISH Tag™ Kit Specifications:

  • Dye (Ex/Em): Alexa Fluor™ 594 (590/615 nm)
  • Comprehensive kit includes 16 reagents
  • Comes with detailed protocol to help ensure your success

Complete Workflow Solution for FISH

FISH Tag™ Kits employ a two-step labeling technology—nick translation to enzymatically incorporate an amine-modified nucleotide (aminoallyl dUTP) followed by chemical labeling with Alexa Fluor™ dyes. PureLink™ nucleic acid purification technology is used for fast and efficient purification of the labeled probe, and SlowFade™ Gold antifade reagent is added to preserve the fluorescent signal during imaging.

More Options for Nucleic Acid Labeling

To review various options for nucleic acid labeling (including DNA and RNA FISH), review Labeling Oligonucleotides and Nucleic Acids—Section 8.2 in the Molecular Probes™ Handbook.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

For Research Use Only. Not for use in diagnostic procedures.
Especificaciones
ColorNaranja
Excitación/emisión555/565 nm
Para utilizar con (aplicación)FISH Tag, fluorescent microscopy, DNA In situ hybridization
N.º de reacciones10 Reactions
Línea de productosAlexa Fluor, FISH Tag
Tipo de productoKit de ensayo de hibridación de ADN in situ
Cantidad1 Kit
Condiciones de envíoHielo seco
ObjetivoADN
Especificidad de dianaSin especificidad
Método de detecciónFluorescencia
Tipo de producto finalProbes (Labeled DNA), cDNA (Labeled)
FormatoKit
Labeling TargetDNA (General), cDNA
Etiqueta o tinteAlexa Fluor™ 555
Tipo de muestraDNA, Tissue (FFPE), Tissue (CRYO), Cell Culture Cells
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador (de -5 a -30 °C) y proteger de la luz.

Preguntas frecuentes

For imaging, what are the limitations of FISH probes?

FISH probes are generally from 300 to 1500 bp in length. To adequately image a gene or other target, the FISH probes must cover a contiguous length of ˜10 kbp. Shorter strand lengths or low-abundance DNA or RNA targets may be detected with the use of fluorescent amplification methods, such as tyramide signal amplification (TSA). For more information using TSA to amplify FISH detection, see this manual (http://tools.thermofisher.com/content/sfs/manuals/mp20912.pdf).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

When should I use an RNA FISH probe versus a DNA FISH probe?

RNA FISH probes are the best option for hybridization to mRNA, rRNA, or other RNA targets. DNA FISH probes can also be used to detect RNA targets, but they do not hybridize as well to mRNA and other RNA target molecules. DNA FISH probes are routinely used to detect gene loci on chromosomes. RNA FISH probes can also be used to detect genes on chromosomes, but DNA FISH probes are more robust for this application.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (1)

Citations & References
Abstract
Aneuploid mosaicism in the developing and adult cerebellar cortex.
Authors:Westra JW, Peterson SE, Yung YC, Mutoh T, Barral S, Chun J,
Journal:J Comp Neurol
PubMed ID:18273885
'Neuroprogenitor cells (NPCs) in several telencephalic proliferative regions of the mammalian brain, including the embryonic cerebral cortex and postnatal subventricular zone (SVZ), display cell division "defects" in normal cells that result in aneuploid adult progeny. Here, we identify the developing cerebellum as a major, nontelencephalic proliferative region of the vertebrate ... More