FM™ 4-64FX, analógico fijable de tinción de membrana de FM™ 4-64
FM™ 4-64FX, analógico fijable de tinción de membrana de FM™ 4-64
Citas y referencias (23)
Invitrogen™

FM™ 4-64FX, analógico fijable de tinción de membrana de FM™ 4-64

La sonda lipofílica FM™ 4-64FX muestra una baja fluorescencia en agua, pero presenta una fluorescencia intensa al unirse a laMás información
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Número de catálogoCantidad
F3465310 x 100 μg
Número de catálogo F34653
Precio (MXN)
-
Cantidad:
10 x 100 μg
La sonda lipofílica FM™ 4-64FX muestra una baja fluorescencia en agua, pero presenta una fluorescencia intensa al unirse a la hoja externa de la membrana plasmática proporcionando una tinción discontinua de la membrana plasmática. La unión es rápida y reversible, y «FX» indica que este proceso analógico se puede fijar mediante fijadores basados en aldehídos. También se trata de un reactivo excelente para identificar activamente neuronas emisoras y para investigar los mecanismos de los ciclos vesiculares dependientes de la actividad.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Colorinfrarrojo
Método de detecciónFluorescente
Para utilizar con (equipo)Microscopio de fluorescencia
Línea de productosFM
Cantidad10 x 100 μg
Condiciones de envíoTemperatura ambiente
Tipo de etiquetaFluorescent Dye
Tipo de productoSonda lipofílica
SubCellular LocalizationMembrana plasmática
Unit SizeEach
Contenido y almacenamiento
Almacenar a temperatura ambiente y proteger de la luz.

Preguntas frecuentes

I want to study endosomes trafficking using FM 4-64. Will the label be retained after fixation? And can I label already-fixed cells?

No. For that you would need the FM 4-64FX version. The non-FX version will not be retained upon fixation, leading to loss of much of the stain and an increase in background. The FX version will be retained using an aldehyde-based fixative. Cells that are already fixed will be stained throughout the cell and the signal will not be localized; it is recommended to stain live cells and then fix.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to study endosome trafficking using FM 4-64 dye. Will the label be retained after fixation? And can I label cells that have already been fixed?

No. For that, you would need the FM 4-64FX version. The non-FX version will be lost, leading to loss of much of the specific label and a vast increase in background labeling. The FX version will be fixed in place with formaldehyde. Cells that have been fixed already will not label correctly, so you will need to label the cells live and then fix.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (23)

Citations & References
Abstract
Crystal structure of the complete integrin alphaVbeta3 ectodomain plus an alpha/beta transmembrane fragment.
Authors:Xiong JP, Mahalingham B, Alonso JL, Borrelli LA, Rui X, Anand S, Hyman BT, Rysiok T, Müller-Pompalla D, Goodman SL, Arnaout MA,
Journal:J Cell Biol
PubMed ID:19704023
'We determined the crystal structure of 1TM-alphaVbeta3, which represents the complete unconstrained ectodomain plus short C-terminal transmembrane stretches of the alphaV and beta3 subunits. 1TM-alphaVbeta3 is more compact and less active in solution when compared with DeltaTM-alphaVbeta3, which lacks the short C-terminal stretches. The structure reveals a bent conformation and ... More
Endocytic trafficking from the small intestinal brush border probed with FM dye.
Authors:Hansen GH, Rasmussen K, Niels-Christiansen LL, Danielsen EM,
Journal:Am J Physiol Gastrointest Liver Physiol
PubMed ID:19679822
The small intestinal brush border functions as the body's main portal for uptake of dietary nutrients and simultaneously acts as the largest permeability barrier against pathogens. To enable this, the digestive enzymes of the brush border are organized in lipid raft microdomains stabilized by cross-linking galectins and intelectin, but little ... More
A stochastic mechanism for biofilm formation by Mycoplasma pulmonis.
Authors:Simmons WL, Bolland JR, Daubenspeck JM, Dybvig K,
Journal:J Bacteriol
PubMed ID:17142389
Bacterial biofilms are communities of bacteria that are enclosed in an extracellular matrix. Within a biofilm the bacteria are protected from antimicrobials, environmental stresses, and immune responses from the host. Biofilms are often believed to have a highly developed organization that is derived from differential regulation of the genes that ... More
Activity-induced rapid synaptic maturation mediated by presynaptic cdc42 signaling.
Authors:Shen W, Wu B, Zhang Z, Dou Y, Rao ZR, Chen YR, Duan S
Journal:Neuron
PubMed ID:16675395
Maturation of presynaptic transmitter secretion machinery is a critical step in synaptogenesis. Here we report that a brief train of presynaptic action potentials rapidly converts early nonfunctional contacts between cultured hippocampal neurons into functional synapses by enhancing presynaptic glutamate release. The enhanced release was confirmed by a marked increase in ... More
Helical disposition of proteins and lipopolysaccharide in the outer membrane of Escherichia coli.
Authors:Ghosh AS, Young KD
Journal:J Bacteriol
PubMed ID:15743937
In bacteria, several physiological processes once thought to be the products of uniformly dispersed reactions are now known to be highly asymmetric, with some exhibiting interesting geometric localizations. In particular, the cell envelope of Escherichia coli displays a form of subcellular differentiation in which peptidoglycan and outer membrane proteins at ... More
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