Portaobjetos preparado n.º 1 FluoCells™ (células BPAE con MitoTracker™ Red CMXRos, Alexa Fluor™ 488-faloidina y DAPI)

Citas y referencias (2)

Invitrogen™

Portaobjetos preparado n.º 1 FluoCells™ (células BPAE con MitoTracker™ Red CMXRos, Alexa Fluor™ 488-faloidina y DAPI)

Número de catálogo	Cantidad
F36924	1 unidad

Número de catálogo F36924

Precio (MXN)

6,959.36

Añadir al carro de la compra

Cantidad:

1 unidad

Precio (MXN)

6,959.36

Añadir al carro de la compra

Los portaobjetos para microscopio preparados FluoCells™ contienen preparaciones celulares multietiqueta fijas para la observación mediante microscopia de fluorescencia. El portaobjetos preparado FluoCells™ n.º 1 contiene células endoteliales de arteria pulmonar bovina (BPAEC). Se ha usado MitoTracker™ Red CMXRos para teñir las mitocondrias de las células vivas, con acumulación dependiente del potencial de la membrana. Después de la fijación y la permeabilización, la F-actina se tiñó con faloidina Alexa Fluor™ 488 y los núcleos se contratiñeron con la tinción de ADN azul fluorescente DAPI.

Consulte nuestra colección completa de reactivos para calibración de microscopios ›

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Para utilizar con (aplicación)Inmunofluorescencia

Cantidad1 unidad

Condiciones de envíoTemperatura ambiente

Línea de productosAlexa Fluor, FluoCells, MitoTracker

TipoPortaobjetos preparados para microscopios

Unit SizeEach

Contenido y almacenamiento

Almacenar a temperatura ambiente y proteger de la luz.

Preguntas frecuentes

Do you have any slides of pre-labeled cells or tissues that I can use to demonstrate with?

Some of the most popular are the FluoCells demonstration slides, which have cells or tissue sections that are labeled with three different dyes. FluoCells 1 and 2 have cultured cells, while FluoCells 3 is a tissue section (mouse kidney). Slides with fluorescent beads are also available.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

DAPI and Hoechst dyes are quite similar to each other. Why would I choose one over the other?

DAPI is a very common blue-fluorescent dye for nuclear counterstaining and gives very bright labeling on nuclei in fixed and permeabilized cells and tissues. However, it is considered to be a semi-permeant to impermeant stain and provides inconsistent staining of live cells. Hoechst 33342 dye is cell-permeant and stains with the same binding mechanism and fluorescent color; it is preferred for live-cell imaging and is just as good as DAPI for fixed cell labeling.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (2)

Citations & References

Abstract

Quality assessment of confocal microscopy slide based systems: performance.

Authors:Zucker RM

Journal:Cytometry A

PubMed ID:16807897

'BACKGROUND: All fluorescence slide-based cytometry detections systems basically include the following components: (1) an excitation light source, (2) intermediate optics, and (3) a detection device consisting of a CCD camera or a PMT. The optical principles employed is slide-based systems are similar to those of confocal microscopes (CLSM). METHODS: The ... More

Quality assessment of confocal microscopy slide-based systems: instability.

Authors:Zucker RM

Journal:Cytometry A

PubMed ID:16807898

'BACKGROUND: All slide-based fluorescence cytometry detections systems basically include an excitation light source, intermediate optics, and a detection device (CCD or PMT). Occasionally, this equipment becomes unstable, generating unreliable and inferior data. METHODS: A number of tests have been devised to evaluate equipment performance and instability. The following four instability ... More