Phusion™ HF and GC Buffer Packs

Name: Phusion™ HF and GC Buffer Packs
SKU: F539L

5X Phusion Green buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel.

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Número de catálogo	Nombre del producto químico o material	Color
F539L	GC Buffer Pack	Green
F518L	HF Buffer Pack	Colorless
F519L	GC Buffer Pack	Colorless
F520L	Detergent-free FF Buffer Pack	Colorless
F521L	Detergent-free GC Buffer Pack	Colorless
F538L	HF Buffer Pack	Green

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Número de catálogo F539L

Precio (MXN)

Nombre del producto químico o material:

GC Buffer Pack

Color:

Green

The error rate of Phusion DNA Polymerase in HF Buffer (4.4 x 10^-7) is lower than that in GC Buffer (9.5 x 10^-7). Therefore, the HF Buffer should be used as the default buffer for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures. For applications such as microarray or DHPLC, where the DNA templates need to be free of detergents, we recommend using detergent-free reaction buffers.

5X Phusion Green Buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffers do not interfere with the enzyme performance and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Two optimized buffers are available for Thermo Scientific Phusion High-Fidelity DNA Polymerases:

5X Phusion HF Buffer (F-518L, F-538L (Phusion Green), F-520L (Detergent-free))
5X Phusion GC Buffer (F-519L, F-539L (Phusion Green), F-521L (Detergent-free))

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Nombre del producto químico o materialGC Buffer Pack

ColorGreen

IncluyeEl paquete de tampones GC Phusion Green (F-539L) incluye:
• Tampón 5X GC Phusion Green (el tampón 1X proporciona 1,5 mm de MgCl₂)
• 50 mm de MgCl₂
• DMSO

Almacénese a -20 °C.

Para utilizar con (aplicación)Standard PCR

Cantidad4 x 1,5 mL

Unit SizeEach

Preguntas frecuentes

Can I use Phusion Green and Phire Green PCR products for subsequent applications such as sequencing, ligation, and restriction digestion?

Yes. The dyes do not interfere with downstream applications such as DNA sequencing, ligation, and restriction digestion.

What dyes are present in Phusion Green and Phire Green buffers?

Phusion Green and Phire Green buffers contain two dyes for monitoring electrophoresis progress. The blue dye migrates with 3-5 kb DNA fragments and the yellow dye migrates faster than 10 bp DNA fragments in 1% agarose gel. The dyes have excitation peaks at 424 nm and 615 nm, respectively.