Reactivo verde de hipoxia para citometría de flujo

El reactivo verde de hipoxia para citometría de flujo es un reactivo independiente de anticuerpos que se utiliza para detectarMás información

Número de catálogo	Cantidad
H20035	2 x 50 ensayos

Número de catálogo H20035

Precio (MXN)

Cantidad:

2 x 50 ensayos

El reactivo verde de hipoxia para citometría de flujo es un reactivo independiente de anticuerpos que se utiliza para detectar niveles bajos de oxígeno en células vivas. La sonda que penetra la membrana libera rodamina a medida que disminuyen los niveles de oxígeno, lo que da como resultado una respuesta fluorogénica. La hipoxia es una característica de muchas enfermedades, incluyendo enfermedades cardiovasculares y de inmunosupresión mediada por tumores y es crítica para la supervivencia y el crecimiento del tumor.

• Ensayo de punto final, fijo y sensible
• Detecta disminuciones de oxígeno en células vivas
• Sonda fluorogénica, no basada en anticuerpos

Especificaciones

Método de detecciónFluorescente

FormatoVial(es)

Cantidad2 x 50 ensayos

Condiciones de envíoTemperatura ambiente

Emission488

Para utilizar con (equipo)Citómetro de flujo

Tipo de productoReactivo

Unit SizeEach

Contenido y almacenamiento

Dos viales de 50 pruebas cada uno

Almacenar entre -5 ° y -30 °C.

Preguntas frecuentes

Can the Hypoxia Green Reagent for Flow Cytometry be used in cells expressing fluorescent proteins?

Yes, as long as the fluorescent protein does not emit in the green range (˜510–535 nm). For cells that do not express a fluorescent protein, we recommend viewing unstained cells under the FITC channel to examine autofluorescence. For cells expressing a fluorescent protein, we recommend analyzing unstained cell samples to determine the extent the fluorescent protein emission may overlap in the FITC channel.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Our Hypoxia Green Reagent for Flow Cytometry is already highly fluorescent before using the product. What could have caused this?

This could be due to storage of the product under anaerobic or low oxygen conditions. The Hypoxia Green Reagent for Flow Cytometry increases in fluorescence upon exposure to low oxygen environments, and this change is not reversible. For some ROS indicators, we recommend storing the reagent under dry nitrogen or argon to prevent oxidation during storage. However, this is not appropriate for the Hypoxia Green Reagent for Flow Cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is different between the use of the Hypoxia Green Reagent for Flow Cytometry (Cat No. H20035) versus imaging using the Image-iT Green Hypoxia Reagent (Cat Nos. I14833, I14834)?

The difference between these products is in the final working concentration of the reagent and incubation time. For flow cytometry applications, we recommend using a final concentration in the range of 0.5 to 1 µM with an incubation time ranging from 2 to 3 hrs. For imaging, we recommend a final concentration in the range of 1 to 10 µM with an incubation time ranging from 30 mins to 1 hr. For both applications, one should optimize the final working concentration and incubation time.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Is Hypoxia Green Reagent for Flow Cytometry (Cat No. H20035) the same reagent as the Image-iT Green Hypoxia Reagent (Cat Nos. I14833, I14834)?

The Hypoxia Green Reagent for Flow Cytometry and the Image-iT Green Hypoxia Reagent are the same reagent, formulated differently for flow cytometry and imaging use, respectively.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (2)

Citations & References

Abstract

Catalase-loaded cisplatin-prodrug-constructed liposomes to overcome tumor hypoxia for enhanced chemo-radiotherapy of cancer.

Authors:Zhang R, Song X, Liang C, Yi X, Song G, Chao Y, Yang Y, Yang K, Feng L, Liu Z

Journal:Biomaterials

PubMed ID:28550753

Aiming at improved therapeutic efficacies, the combination of chemotherapy and radiotherapy (chemo-radiotherapy) has been widely studied and applied in clinic. However, the hostile characteristics of tumor microenvironment such as hypoxia often limit the efficacies in both types of cancer therapies. Herein, catalase (CAT), an antioxidant enzyme, is encapsulated inside liposomes ... More

Tumor-targeted nanoplatform for in situ oxygenation-boosted immunogenic phototherapy of colorectal cancer.

Authors:He H, Liu L, Liang R, Zhou H, Pan H, Zhang S, Cai L

Journal:Acta Biomater

PubMed ID:31945508

Advanced colorectal cancer has a high mortality rate since conventional treatments have limited therapeutic effects and poor prognosis with high risks of metastasis and recurrence. Photodynamic therapy (PDT) is a promising treatment modality for the eradication of colorectal cancer, but its curative efficacy is severely affected by tumor hypoxia. Herein, ... More