Image-IT™ DEAD Green™ Viability Stain
Image-IT™ DEAD Green™ Viability Stain
Citas y referencias (6)
Invitrogen™

Image-IT™ DEAD Green™ Viability Stain

La tinción de viabilidad verde Image-iT® DEAD™ es un indicador de viabilidad rápido, sensible y fácil de usar que tambiénMás información
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Número de catálogoCantidad
I102911 vial
Número de catálogo I10291
Precio (MXN)
-
Cantidad:
1 vial
La tinción de viabilidad verde Image-iT® DEAD™ es un indicador de viabilidad rápido, sensible y fácil de usar que también es susceptible de fijación y permeabilidad, lo que permite el multiplexing con otros biomarcadores de citotoxicidad. El colorante verde Image-iT® DEAD™ es impermeable a las células sanas y se vuelve permeable cuando la membrana plasmática de las células está en riesgo.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Concentración1 mM
Método de detecciónFluorescente
FormularioLíquido
Cantidad1 vial
Condiciones de envíoHielo húmedo
Localización subcelularNucleus
ColorVerde
EmissionVisible
Para utilizar con (aplicación)Ensayo de viabilidad
Para utilizar con (equipo)Microscopio de fluorescencia
Línea de productosImage-iT, Molecular Probes
Tipo de productoTinción de viabilidad
Unit SizeEach
Contenido y almacenamiento
La tinción incluye material suficiente para realizar 10 placas en formato de placa de 96 pocillos y 50 μl de volumen de pocillos.
  • Almacenar a ≤ −20 °C, desecar y proteger de la luz.

    • Preguntas frecuentes

    Do you offer a cell viability stain compatible with fixation?

    Our Image-IT DEAD Green Viability Stain (Cat. No. I10291) is amenable to fixation and permeabilization after the application of the stain. It is important to use fixatives free of alcohol or other organic solvents.

    Find additional tips, troubleshooting help, and resources within our Cell Viability, Proliferation, Cryopreservation, and Apoptosis Support Center.

    Which cell viability kits are compatible with fixation?

    The LIVE/DEAD Fixable kits for flow cytometry analysis are compatible with fixation. These kits use amine-reactive cell-impermeant dyes that stain the cell surface of live cells and also the cytosol of dead cells-live cells are dim and dead cells are bright. Since the dye is covalently bound to the cells, it will be retained after fixation. Unfortunately, this method does not work well for imaging-based assays, as all cells are stained and it is difficult to distinguish bright dead cells from dim live cells with a microscope. Ethidium monoazide (EMA; Cat No. E1374) is a cell impermeant nucleic acid stain that can be applied to live cultures and stains only dead cells. After incubation and washing away unbound dye, the cells can be exposed to light to photoactivate EMA to crosslink to dead cell DNA. After crosslinking to dead cell DNA, the samples may be fixed and permeabilized. Image-IT DEAD Green Viability Stain (Cat. No. I10291) for imaging and high-content screening (HCS) analysis is a live-cell impermeant DNA binding dye that is compatible with fixation and permeabilization with good retention up to 48 hours. We also have a LIVE/DEAD Reduced Biohazard Cell Viability Kit (Cat. No. L7013) for imaging and flow analysis that contains two DNA binding dyes, SYTO 10 and Dead Red, that are sufficiently retained to be analyzed soon after 4% glutaraldehyde fixation.
    Note: In general, DNA-binding dyes and calcein AM are not compatible with fixation, as these dyes are not covalently bound to components of the cell and will thus slowly diffuse out of cells after fixation, gradually staining all cells as dead.

    Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

    Citations & References (6)

    Citations & References
    Abstract
    Global metabolic profiling of infection by an oncogenic virus: KSHV induces and requires lipogenesis for survival of latent infection.
    Authors:Delgado T, Sanchez EL, Camarda R, Lagunoff M,
    Journal:PLoS Pathog
    PubMed ID:22916018
    Like cancer cells, virally infected cells have dramatically altered metabolic requirements. We analyzed global metabolic changes induced by latent infection with an oncogenic virus, Kaposi's Sarcoma-associated herpesvirus (KSHV). KSHV is the etiologic agent of Kaposi's Sarcoma (KS), the most common tumor of AIDS patients. Approximately one-third of the nearly 200 ... More
    Antibody-targeted nanovectors for the treatment of brain cancers.
    Authors:Sharpe MA, Marcano DC, Berlin JM, Widmayer MA, Baskin DS, Tour JM,
    Journal:ACS Nano
    PubMed ID:22390360
    Introduced here is the hydrophilic carbon clusters (HCCs) antibody drug enhancement system (HADES), a methodology for cell-specific drug delivery. Antigen-targeted, drug-delivering nanovectors are manufactured by combining specific antibodies with drug-loaded poly(ethylene glycol)-HCCs (PEG-HCCs). We show that HADES is highly modular, as both the drug and antibody component can be varied ... More
    Analysis of cell cycle and replication of mouse macrophages after in vivo and in vitro Cryptococcus neoformans infection using laser scanning cytometry.
    Authors:Coelho C, Tesfa L, Zhang J, Rivera J, Gonçalves T, Casadevall A,
    Journal:Infect Immun
    PubMed ID:22252872
    We investigated the outcome of the interaction of Cryptococcus neoformans with murine macrophages using laser scanning cytometry (LSC). Previous results in our lab had shown that phagocytosis of C. neoformans promoted cell cycle progression. LSC allowed us to simultaneously measure the phagocytic index, macrophage DNA content, and 5-ethynyl-2'-deoxyuridine (EdU) incorporation ... More
    Induction of the Warburg effect by Kaposi's sarcoma herpesvirus is required for the maintenance of latently infected endothelial cells.
    Authors:Delgado T, Carroll PA, Punjabi AS, Margineantu D, Hockenbery DM, Lagunoff M,
    Journal:Proc Natl Acad Sci U S A
    PubMed ID:20498071
    Kaposi's sarcoma (KS) is the most commonly reported tumor in parts of Africa and is the most common tumor of AIDS patients world-wide. KS-associated herpesvirus (KSHV) is the etiologic agent of KS. Although KS tumors contain many cell types, the predominant cell is the spindle cell, a cell of endothelial ... More
    Miniaturized modular-array fluorescence microscopy.
    Authors:
    Journal:Biomed Opt Express
    PubMed ID:33408992
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