ImaGene Red™ C12RG lacZ Gene Expression Kit
ImaGene Red&trade; C<sub>12</sub>RG <i>lacZ</i> Gene Expression Kit
Citas y referencias (4)
Invitrogen™

ImaGene Red™ C12RG lacZ Gene Expression Kit

El kit de expresión de genes lacZ ImaGene Red™ contiene un sustrato de galactosidasa a base de fluoresceína que seMás información
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Número de catálogoCantidad
I29061 kit
Número de catálogo I2906
Precio (MXN)
-
Cantidad:
1 kit
El kit de expresión de genes lacZ ImaGene Red™ contiene un sustrato de galactosidasa a base de fluoresceína que se ha modificado covalentemente para incluir una fracción lipofílica de 12 carbonos, C12RG. Una vez dentro de la célula, los sustratos son escindidos por la β-galactosidasa, generando un producto fluorescente que es bien retenido por las células, probablemente por la incorporación de la cola lipofílica dentro de la membrana celular. Además de la C12RG, los kits también incluyen el inhibidor de la β-galactosidasa de amplio espectro, PETG, y difosfato de cloroquina para inhibir la hidrólisis ácida del sustrato.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
ensayoEnsayo de β-Gal (lacZ)
Método de detecciónFluorescente
Línea de productosDetectaGene
Tipo de productoSistema de ensayo de gen indicador de β-galactosidasa
Cantidad1 kit
Condiciones de envíoTemperatura ambiente
SustratoC12RG
Propiedades de sustratoSustrato químico
Tipo de sustratoSustrato Beta-Gal
Enzima dianaBeta-galactosidasa
FormatoKit
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador (de -5 a -30 °C) y proteger de la luz.

Citations & References (4)

Citations & References
Abstract
Vital staining of cardiac myocytes during embryonic stem cell cardiogenesis in vitro.
Authors:Metzger JM, Lin WI, Samuelson LC,
Journal:Circ Res
PubMed ID:8635211
'Mouse embryonic stem (ES) cells differentiate in vitro into a variety of cell types, including spontaneously contracting cardiac myocytes. The primary aim of this work was to use vital stain techniques for real-time detection of developing cardiac myocytes in ES cell differentiation cultures. The -440 to +6 human cardiac alpha-actin ... More
Activity of Hb9 interneurons during fictive locomotion in mouse spinal cord.
Authors:Kwan AC, Dietz SB, Webb WW, Harris-Warrick RM,
Journal:J Neurosci
PubMed ID:19759307
'Hb9 interneurons (Hb9 INs) are putative components of the mouse spinal locomotor central pattern generator (CPG) and candidates for the rhythm-generating kernel. Studies in slices and hemisected spinal cords showed that Hb9 INs display TTX-resistant membrane potential oscillations, suggesting a role in rhythm generation. To further investigate the roles of ... More
Functional ephrin-B2 expression for promotive interaction between arterial and venous vessels in postnatal neovascularization.
Authors:Hayashi S, Asahara T, Masuda H, Isner JM, Losordo DW,
Journal:Circulation
PubMed ID:15851594
'Ephrin-B2, one of the transmembrane ligands, is a genetic marker of arterial endothelial cells (ECs) at embryonic stages and is essential for cardiovascular development, but its roles in ischemic cardiovascular disease are not well understood. In this study, we focused on the function of ephrin-B2 in postnatal neovascularization. We found ... More
Fluorescence-activated cell sorting (FACS) of Drosophila hemocytes reveals important functional similarities to mammalian leukocytes.
Authors:Tirouvanziam R, Davidson CJ, Lipsick JS, Herzenberg LA
Journal:Proc Natl Acad Sci U S A
PubMed ID:14976247
Drosophila is a powerful model for molecular studies of hematopoiesis and innate immunity. However, its use for functional cellular studies remains hampered by the lack of single-cell assays for hemocytes (blood cells). Here we introduce a generic method combining fluorescence-activated cell sorting and nonantibody probes that enables the selective gating ... More