Reactivo Image-iT™ TMRM (indicador de potencial de membrana mitocondrial)

Citas y referencias (5)

Invitrogen™

Reactivo Image-iT™ TMRM (indicador de potencial de membrana mitocondrial)

Las membranas mitocondriales sanas mantienen una diferencia en el potencial eléctrico entre el interior y el exterior del orgánulo, conocidoMás información

Número de catálogo	Cantidad
I34361	5 x 100 μL

Número de catálogo I34361

Precio (MXN)

6,741.79

Añadir al carro de la compra

Cantidad:

5 x 100 μL

Precio (MXN)

6,741.79

Añadir al carro de la compra

Las membranas mitocondriales sanas mantienen una diferencia en el potencial eléctrico entre el interior y el exterior del orgánulo, conocido como potencial de membrana. La tetrametilrodamina, éster metílico (TMRM) es una tinción que penetra en las células y se acumula en las mitocondrias activas con potenciales de membrana intactos. Si las células están sanas y tienen mitocondrias en funcionamiento, la señal será brillante. Tras la pérdida del potencial de la membrana mitocondrial, la acumulación de TMRM cesará y la señal se atenuará o desaparecerá. La señal de TMRM se puede detectar con microscopía de fluorescencia, citometría de flujo, clasificación celular, detección de alto rendimiento y análisis de alto contenido.

• Para el estudio dinámico de células vivas del potencial de la membrana mitocondrial (no fijable)
• Detectable con el conjunto de filtros RFP/TRITC (pico de absorción: 548 nm, pico de emisión: 574 nm)
• Multiplexable con tinciones de emisión verde, rojo profundo, violeta y azul, como GFP, DAPI, Hoechst, HCS LipidTOX y LysoTracker Deep Red

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Cantidad5 x 100 μL

Condiciones de envíoHielo húmedo

ColorNaranja

Línea de productosMolecular Probes

Tipo de productoReactivo

Unit SizeEach

Contenido y almacenamiento

Almacenar en el congelador de -5 °C a -30 °C y proteger de la luz.

Preguntas frecuentes

For a final staining solution, we can add TMRM directly into cell growth medium. Can this media contain serum?

Yes, you may add TMRM to media with serum. Serum components may bind dye non-specifically so you may need a higher final working concentration for staining samples in media containing serum relative to staining the same cells in a non-serum media or buffer.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How is TMRM different from the MitoTracker dyes?

TMRM is a dynamic mitochondrial stain. There is no covalent attachment of TMRM to the mitochondria and because of this, it can move in and out of the mitochondria dependent upon changes in the membrane potential. The MitoTracker dyes on the other hand possess a reactive chloromethyl group that allows for their covalent attachment to mitochondria. As a result, once they accumulate within mitochondria that have a normal membrane potential, they are retained.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is included in the Image-iT TMRM Reagent (Cat No. I34361)?

The Image-iT TMRM Reagent is a ready-to-use 1,000X concentrated stock solution of the TMRM dye. It contains 5 vials of 100 µM of TMRM in neat DMSO, with 100 µL volume in each vial. TMRM powder (Cat No. T668) is not included in this kit.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (5)

Citations & References

Abstract

miR-450a Acts as a Tumor Suppressor in Ovarian Cancer by Regulating Energy Metabolism.

Authors:Muys BR, Sousa JF, Plaça JR, de Araújo LF, Sarshad AA, Anastasakis DG, Wang X, Li XL, de Molfetta GA, Ramão A, Lal A, Vidal DO, Hafner M, Silva WA

Journal:Cancer Res

PubMed ID:31101765

'Dysregulation of miRNA expression is associated with multiple diseases, including cancers, in which small RNAs can have either oncogenic or tumor suppressive functions. Here we investigated the potential tumor suppressive function of miR-450a, one of the most significantly downregulated miRNAs in ovarian cancer. RNA-seq analysis of the ovarian cancer cell ... More

Ursodeoxycholic acid protects against cisplatin-induced acute kidney injury and mitochondrial dysfunction through acting on ALDH1L2.

Authors:Yang Y, Liu S, Gao H, Wang P, Zhang Y, Zhang A, Jia Z, Huang S

Journal:Free Radic Biol Med

PubMed ID:32004633

'Mitochondrial dysfunction plays an important role in acute kidney injury (AKI). Thus, the agents improving the mitochondrial function could be beneficial for treating AKI. Ursodeoxycholic acid (UDCA) has been demonstrated to prevent mitochondrial dysfunction under pathology, however, its role in AKI and the underlying mechanism remain unknown. This study aimed ... More

Targeting mitochondria-associated membranes as a potential therapy against endothelial injury induced by hypoxia.

Authors:Yang YD, Li MM, Xu G, Zhang EL, Chen J, Sun B, Chen DW, Gao YQ

Journal:J Cell Biochem

PubMed ID:31241212

Mitochondrial dysfunction plays a principal role in hypoxia-induced endothelial injury, which is involved in hypoxic pulmonary hypertension and ischemic cardiovascular diseases. Recent studies have identified mitochondria-associated membranes (MAMs) that modulate mitochondrial function under a variety of pathophysiological conditions such as high-fat diet-mediated insulin resistance, hypoxia reoxygenation-induced myocardial death, and hypoxia-evoked vascular smooth ... More

Barrier-to-Autointegration Factor 1 Protects against a Basal cGAS-STING Response.

Authors:Ma H, Qian W, Bambouskova M, Collins PL, Porter SI, Byrum AK, Zhang R, Artyomov M, Oltz EM, Mosammaparast N, Miner JJ, Diamond MS

Journal:mBio

PubMed ID:32156810

Although the pathogen recognition receptor pathways that activate cell-intrinsic antiviral responses are well delineated, less is known about how the host regulates this response to prevent sustained signaling and possible immune-mediated damage. Using a genome-wide CRISPR-Cas9 screening approach to identify host factors that modulate interferon-stimulated gene (ISG) expression, we identified ... More

Mitochondrial Pyruvate Carrier 1 Promotes Peripheral T Cell Homeostasis through Metabolic Regulation of Thymic Development.

Authors:Ramstead AG, Wallace JA, Lee SH, Bauer KM, Tang WW, Ekiz HA, Lane TE, Cluntun AA, Bettini ML, Round JL, Rutter J, O'Connell RM

Journal:Cell Rep

PubMed ID:32130894

Metabolic pathways regulate T cell development and function, but many remain understudied. Recently, the mitochondrial pyruvate carrier (MPC) was identified as the transporter that mediates pyruvate entry into mitochondria, promoting pyruvate oxidation. Here we find that deleting Mpc1, an obligate MPC subunit, in the hematopoietic system results in a specific reduction ... More