Pilas de transferencia iBlot™, nitrocelulosa, tamaño normal

Name: Pilas de transferencia iBlot™, nitrocelulosa, tamaño normal
SKU: IB301001

Las pilas de transferencia iBlot™ se utilizan para transferir proteínas mediante el dispositivo de transferencia de gel iBlot™. Las pilasMás información

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Número de catálogo	Cantidad	Dimensions (LxW)
IB301001	10 sets	Normal, 13,5 cm x 8 cm
IB301031	3 x 10 sets	Normal, 13,5 cm x 8 cm

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Número de catálogo IB301001

Precio (MXN)

Cantidad:

10 sets

Dimensions (LxW):

Normal, 13,5 cm x 8 cm

Las pilas de transferencia iBlot™ se utilizan para transferir proteínas mediante el dispositivo de transferencia de gel iBlot™. Las pilas de transferencia iBlot™ son pilas desechables que se suministran en tres variedades para diferentes aplicaciones: las pilas de transferencia de gel iBlot™ incluyen membranas PVDF o de transferencia de nitrocelulosa para realizar secante seco de proteínas, las pilas de transferencia de ADN iBlot™ tienen una membrana de transferencia de nailon integrada para realizar secante seco de ADN, y las pilas de detección Western iBlot™ se utilizan para realizar detección Western en membranas PVDV o de nitrocelulosa secadas previamente. Cada pila de transferencia iBlot™ contiene un electrodo de cobre y tampones de cátodo y ánodo adecuados en la matriz de gel para permitir transferencias rápidas y fiables de proteínas o ADN.

Membrana de nitrocelulosa
La membrana de nitrocelulosa (0,2 µm de tamaño de poro) incluida en esta pila está compuestas de nitrocelulosa 100 % pura para proporcionar una transferencia de alta calidad. La membrana es compatible con los métodos de detección más utilizados como tinción, inmunodetección, fluorescencia o marcado radiactivo. Las proteínas se unen a la membrana debido a interacciones hidrófobas y electrostáticas. La capacidad de unión de las proteínas es de 209 µg/cm².

Tamaños de pilas de transferencia
El tamaño normal (13 cm x 8,3 cm) se utiliza para todos los tipos de gel midi Novex™ (o equivalentes) y geles E-PAGE™ 48 o 96. El tamaño mini (8 cm x 8 cm) se utiliza para todos los minigeles Novex™ (o equivalentes).

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Características ecológicasEliminación sostenible

Cantidad10 sets

Condiciones de envíoTemperatura ambiente

Dimensions (LxW)Normal, 13,5 cm x 8 cm

FormatoPila de transferencia

Longitud (métrico)13,5 cm

MaterialNitrocelulosa

Tamaño de poro0,2 μm

Línea de productosiBlot

Suficiente para10 transferencias

Anchura (métrico)8 cm

Unit SizeEach

Contenido y almacenamiento

10 juegos de pilas de transferencia en una caja. Almacenar a temperatura ambiente.

Preguntas frecuentes

Following the release of the iBlot 2 Gel Transfer Device, are consumables for the original iBlot Gel Transfer Device still available?

Yes, all original iBlot stacks are still available for purchase. You can find them in the original iBlot Gel Transfer Device Reagents and Resources (http://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/transfer-proteins-western-blot/iblot-dry-blotting-system/reagents-resources-original-iblot-gel-transfer-device.html). However, please note that they are not compatible with the iBlot 2 Gel transfer Device.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Is the iBlot 2 Gel Transfer Device compatible with original iBlot Transfer Stacks?

No. Do not use iBlot Transfer Stacks in the iBlot 2 Gel Transfer Device, and do not mix components between iBlot Transfer Stacks and iBlot 2 Transfer Stacks.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

How can I get better transfer of high-molecular weight proteins?

Proteins larger than ~150 kDa migrate more slowly than smaller proteins. Therefore, more time is required to transfer them from gel to membrane. We recommend extending the transfer time by 8 to 10 minutes for optimal transfer of proteins >150 kDa using the iBlot Dry Blotting System.

To enhance transfer efficiency, we also recommend adding an equilibration (gel-soaking) step between electrophoresis and western transfer and using NuPAGE Invitrogen 3 - 8% Tris-acetate gels for electrophoresis. We have an application note available, titled “Transferring Large and Small Proteins Using the iBlot Dry Blotting System”. To download the PDF, see the iBlot Reagents Resources page. (http://www.thermofisher.com/us/en/home/life-science/protein-expression-and-analysis/western-blotting/western-blot-transfer/iblot-dry-blotting-system/original-iblot-resources.html)

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Occasionally my western blots have high background. What do you recommend?

This may be a result of insufficient blocking or nonspecific binding. We suggest trying our WesternBreeze Blocker/Diluent (Cat. No. WB7050). We have been using it with good success. Additionally, you should optimize primary and secondary antibody concentrations as generally recommended for any new blotting technique. Many cases of high background can be resolved by further diluting one or both antibody preparations.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Is it possible to substitute the membrane from the iBlot Transfer Stack with my specialized membrane?

In theory, you can replace the membrane provided in your iBlot Transfer Stack with any membrane that is compatible with western blotting. To do this, cut the alternative membrane to match the size of your gel, and wet the membrane. Then, either place the alternative membrane on top of the integrated membrane, or carefully remove the integrated membrane from the gel matrix with forceps and replace it with the new membrane. Note that we only support the use of iBlot Transfer Stacks when they are used with the provided instructions.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

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