Low Density Lipoprotein from Human Plasma, pHrodo™ Green- and Red-LDL Conjugates

Detect low density lipoprotein (LDL) uptake, endosomal maturation, and trafficking in cells with pHrodo Green-LDL and pHrodo Red-LDL conjugates. These LDL conjugates contain pH-sensitive pHrodo dyes that are weakly fluorescent at neutral pH, yet fluoresce brightly upon exposure to acidic environments, such as those found in endosomes. The pHrodo Green-LDL and Red-LDL conjugates can be used in fluorescence microscopy, flow cytometry, high-throughput screening, and high content analysis experiments.

Human low density lipoprotein (LDL) is one of the key lipid:protein complexes in blood and is a crucial component of metabolism responsible for the transport of lipids throughout the body. LDL from human plasma, when complexed with pHrodo Green dye (pHrodo Green-LDL) or pHrodo Red dye (pHrodo Red-LDL), is a valuable tool in studies of LDL uptake through endocytosis and trafficking of LDL throughout the cell. pHrodo Green-LDL and pHrodo Red-LDL are dimly fluorescent at neutral pH outside of cells but fluoresce brightly after endocytosis. This feature allows for a no-wash protocol when using pHrodo Green-LDL or pHrodo Red-LDL, enabling kinetic measurement of LDL uptake, endosomal maturation, and trafficking in cells. These fluorescently labeled LDL conjugates have been designed for use with fluorescence microscopy, flow cytometry, cell sorting, high-throughput analysis, and high content analysis.

Features of pHrodo Green-LDL:
• Detectable with GFP/FITC filter set (absorption peak: ∼509 nm, emission peak: ∼533 nm)
• Mutiplexable with a wide variety of blue, red, and far-red dyes reporters such as Mitosox Red, CellEvent Caspase 3/7 Red, NucBlue, RFPs, and Mitotracker Deep Red, among many others.

Features of pHrodo Red-LDL:
• Detectable with RFP/TRITC filter set (absorption peak: ∼560 nm, emission peak: ∼585 nm)
• Mutiplexable with a wide variety of blue, green, and far red dyes and reporters such as GFP, Fuo-4, calcein, NucBlue, CellEvent Caspase 3/7 green, Mitosox Green, and Mitotracker Deep Red, among many others.

Both pHrodo Green-LDL and pHrodo Red-LDL can be used in a no-wash protocol, allowing dynamic measurement of LDL uptake and cell trafficking.