LIVE/DEAD™ Cell Vitality Assay Kit, C12 Resazurin/SYTOX™ Green
LIVE/DEAD&trade; Cell Vitality Assay Kit, C<sub>12</sub> Resazurin/SYTOX&trade; Green
Citas y referencias (4)
Invitrogen™

LIVE/DEAD™ Cell Vitality Assay Kit, C12 Resazurin/SYTOX™ Green

El kit de ensayo de vitalidad celular LIVE/DEAD proporciona un ensayo sencillo de fluorescencia de dos colores que distingue lasMás información
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Número de catálogoCantidad
L349511000 ensayos
Número de catálogo L34951
Precio (MXN)
-
Cantidad:
1000 ensayos
El kit de ensayo de vitalidad celular LIVE/DEAD proporciona un ensayo sencillo de fluorescencia de dos colores que distingue las células activas metabólicas de las células lesionadas y las células muertas. El ensayo se basa en la reducción de C12-resazurina a C12-resorufina de color rojo fluorescente en células metabólicamente activas y en la captación de tinción de ácido nucleico de color verde fluorescente e impenetrable en células, el colorante SYTOX Green, en células con membranas plasmáticas comprometidas (generalmente, células apoptóticas y necróticas tardías). En este ensayo, las células muertas emiten sobre todo fluorescencia verde y las células sanas y metabólicamente activas emiten sobre todo fluorescencia roja; las células lesionadas presentan una disminución de la fluorescencia roja y verde.

Consulte una guía de selección para todos los ensayos de viabilidad celular para la citometría de flujo.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Permeabilidad celularImpermeable, permeable
Tipo de célulaCélulas de mamíferos, células eucariotas
DescripciónKit de ensayo de vitalidad celular LIVE/DEAD™, C12 resazurina/SYTOX™ Green
Método de detecciónFluorescente
Tipo de coloranteOtras etiquetas o colorantes
FormatoTubo(s)
Cantidad1000 ensayos
Condiciones de envíoTemperatura ambiente
SolubilidadDMSO (dimetilsulfóxido)
ColorVerde, rojo
EmissionC12 resazurina: 563/587 nm
SYTOX™ Green: 504/523 nm
Excitation Wavelength Range504/563 nm
Para utilizar con (aplicación)Ensayo de viabilidad
Para utilizar con (equipo)Microscopio de fluorescencia, Citómetro de flujo, Lector de microplacas
Línea de productosLIVE/DEAD, SYTOX
Tipo de productoKit de ensayo de vitalidad celular
Unit SizeEach
Contenido y almacenamiento
Contiene 5 viales de C12-resazurina (40 μg en cada vial), 1 vial de SYTOX™ Green (100 μl, solución de 10 μM en DMSO), 1 vial de DMSO (1,5 ml) y tampón de fosfato 10X (100 ml).

Almacenar en el congelador (de -5 °C a -30 °C) y proteger de la luz.

Preguntas frecuentes

How do SYTO dyes bind to DNA?

The binding mode of SYTO nucleic acid stains is unknown. However, the behavior of these and related nucleic acid dyes suggests the following binding properties:

1.They appear to contact the solvent (suggested by sensitivity to salt, divalent cations, and in particular, SDS) and thus are likely to have contacts in the grooves.
2.All SYTO dyes appear to show some base selectivity and are thus likely to have minor groove contacts.
3.They can be removed from nucleic acid via ethanol precipitation; this characteristic is not shared by ethidium bromide and other intercalators. Likewise, the dyes are not removed from nucleic acid via butanol or chloroform extraction. These extraction methods do remove ethidium bromide from nucleic acid. 4. SYTO binding is not affected by nonionic detergents.
5. SYTO dyes are not quenched by BrdU, so they do not bind nucleic acids in precisely the same way as Hoechst 33342 and DAPI ((4′,6-diamidino-2-phenylindole).

SYBR Green I has shown little mutagenicity on frameshift indicator strains, indicating that it isn't likely to strongly intercalate.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How do alamarBlue reagent and PrestoBlue reagent differ from resazurin and C12-resazurin?

alamarBlue reagent and PrestoBlue reagent contain resazurin in a proprietary stabilizing formulation that allows for a convenient “mix, incubate, and read” protocol. PrestoBlue reagent is an improvement in the formulation of alamarBlue reagent that allows for much faster staining (typically 10 minutes vs. 1-4 hours to obtain a similar signal and sensitivity). C12-resazurin is a derivative of resazurin that has better cellular retention and thus allows for analysis on a flow cytometer and multiplexing with viability indicators and other biomarkers.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How do I prepare dead cell controls for LIVE/DEAD cell viability assays?

There are two easy options. One is to heat-inactivate the cells by placing at 60 degrees C for 20 minutes. The second is to subject the cells to 70% ethanol. Alcohol-fixed cells can be stored indefinitely in the freezer until use, potentially up to several years.

Centrifuge cells, pellet, and remove supernatant.
Fix cells: Add 10 mL ice cold 70% ETOH to a 15 mL tube containing the cell pellet, adding dropwise at first while vortexing, mix well.
Store in freezer until use.
When ready to use, wash twice and resuspend in buffer of choice.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (4)

Citations & References
Abstract
Titanium-Enriched Hydroxyapatite-Gelatin Scaffolds with Osteogenically Differentiated Progenitor Cell Aggregates for Calvaria Bone Regeneration.
Authors:Ferreira JR, Padilla R, Urkasemsin G, Yoon K, Goeckner K, Hu WS, Ko CC,
Journal:Tissue Eng Part A
PubMed ID:23495972
'Adequate bony support is the key to re-establish both function and esthetics in the craniofacial region. Autologous bone grafting has been the gold standard for regeneration of problematic large bone defects. However, poor graft availability and donor-site complications have led to alternative bone tissue-engineering approaches combining osteoinductive biomaterials and three-dimensional ... More
Local phagocytic responses after murine infection with different forms of Fonsecaea pedrosoi and sclerotic bodies originating from an inoculum of conidiogenous cells.
Authors:Machado AP, Silva MR, Fischman O,
Journal:Mycoses
PubMed ID:19925569
'Fonsecaea pedrosoi is an important causative agent of chromoblastomycosis (CBM) especially in humid areas of the world; however, little is known about the infective forms of this agent that cause CBM. The aim of this study was to investigate the murine tissue response to inoculation with different forms of F. ... More
Prolonged infection by Fonsecaea pedrosoi after antigenic co-stimulation at different sites in experimental murine chromoblastomycosis.
Authors:Machado AP, Silva MR, Fischman O,
Journal:Virulence
PubMed ID:21178410
'In the present study, we examined prolonged infection after antigenic co-stimulation by inoculation of the fungus Fonsecaea pedrosoi at two different sites in three mouse strains (BALB/c, Swiss, and C57BL/6). Using this murine model of infection, we showed that antigen induction of infection at more than one site led to ... More
Radio frequency radiation causes no nonthermal damage in enzymes and living cells.
Authors:Fortune JA, Wu BI, Klibanov AM,
Journal:Biotechnol Prog
PubMed ID:20572294
The ability of radio frequency radiation (RFR) to exert irreversible nonthermal (i.e., not caused by accompanying heat) effects on biologics has been widely debated due to a relative paucity of comprehensive critical details in published reports dealing with this issue. In this study, we used rigorous control over experimental conditions ... More