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Citas y referencias (702)
Invitrogen™
Lucifer Yellow CH, Lithium Salt, 25 mg
Sal de litio de carbohidrazida (CH) de color amarillo lucifer es un colorante soluble en agua con picos de excitaciónMás información
| Número de catálogo | Cantidad |
|---|---|
| L453 | 25 mg |
Número de catálogo L453
Precio (MXN)
-
Cantidad:
25 mg
Sal de litio de carbohidrazida (CH) de color amarillo lucifer es un colorante soluble en agua con picos de excitación y emisión de 428 y 536 nm respectivamente. Es la herramienta preferida para el estudio de la morfología neuronal, ya que contiene un grupo de carbohidrazida (CH) que permite que se ligue covalentemente a las biomoléculas circundantes durante la fijación con aldehído. La forma de sal de litio del CH amarillo lucifer se utiliza comúnmente para la microinyección porque tiene mayor solubilidad que las formas salinas de amonio y potasio de CH amarillo lucifer.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
ColorAmarillo
Método de detecciónFluorescencia
Para utilizar con (equipo)Microscopio de fluorescencia
Peso molecular457.24
Tipo de productoSal de litio
Cantidad25 mg
Condiciones de envíoTemperatura ambiente
Localización subcelularCitoplasma & citosol
Excitation/Emission428/536 nm
Unit SizeEach
Contenido y almacenamiento
Almacenar a temperatura ambiente y proteger de la luz.
Citations & References (702)
Citations & References
Abstract
Endothelial and smooth muscle cell conduction in arterioles controlling blood flow.
Journal:Am J Physiol
PubMed ID:9458866
We performed intracellular recording with Lucifer yellow dye microinjection to investigate the cellular pathway(s) by which constriction and dilation are conducted along the wall of arterioles (diameter 47 +/- 1 microns, n = 63) supplying blood flow to the cheek pouch of anesthetized hamsters. At rest, membrane potential (Em) of
Bursting response to current-evoked depolarization in rat CA1 pyramidal neurons is correlated with lucifer yellow dye coupling but not with the presence of calbindin-D28k.
Journal:Synapse
PubMed ID:2042109
Calbindin-D28k (CaBP) immunohistochemistry has been combined with electrophysiological recording and Lucifer Yellow (LY) cell identification in the CA1 region of the rat hippocampal formation. CaBP is shown to be contained within a distinct sub-population of CA1 pyramidal cells which is equivalent to the superficial layer described by Lorente de Nó
Membrane conductance oscillations in astrocytes induced by phorbol ester.
Journal:Nature
PubMed ID:3627267
Glial cells in the central nervous systems (CNS) have complex functions which are difficult to decipher because of the intimate intertwining of glial cells with neurons. We have therefore developed an essentially neuron-free preparation of CNS astrocytes in the kainic acid lesioned hippocampal slice. With this preparation we have examined
Deformation of network connectivity in the inferior olive of connexin 36-deficient mice is compensated by morphological and electrophysiological changes at the single neuron level.
Journal:J Neurosci
PubMed ID:12805309
Compensatory mechanisms after genetic manipulations have been documented extensively for the nervous system. In many cases, these mechanisms involve genetic regulation at the transcription or expression level of existing isoforms. We report a novel mechanism by which single neurons compensate for changes in network connectivity by retuning their intrinsic electrical
Dual fluorescence combined with a two-color immunoperoxidase technique: a new way of visualizing diverse neuronal elements.
Journal:J Neurosci Methods
PubMed ID:1712061
'A method is described that allows an estimation of the neurotransmitter-related immunoreactivity, morphology and relationship to other immunoreactive elements, of single functionally identified neurons in the central nervous system. First, neurons are identified electrophysiologically using intracellular recording and labelled by iontophoresis of lucifer yellow (LY). After fixation and sectioning of