Marcadores de peso molecular de proteínas preteñidos Novex™
Marcadores de peso molecular de proteínas preteñidos Novex™
Invitrogen™

Marcadores de peso molecular de proteínas preteñidos Novex™

El estándar de proteínas preteñido Novex Sharp se ha diseñado para una estimación del peso molecular práctica, sencilla y precisaMás información
Have Questions?
Número de catálogoCantidad
LC58002 x 250 μl
Número de catálogo LC5800
Precio (MXN)
-
Cantidad:
2 x 250 μl
Pedido a granel o personalizado
El estándar de proteínas preteñido Novex Sharp se ha diseñado para una estimación del peso molecular práctica, sencilla y precisa de una amplia variedad de proteínas de peso molecular durante la SDS-PAGE y Western blot. El estándar consta de 12 bandas de colores en el rango de 3,5 a 260 kDa y se suministra en un formato listo para su uso para la carga directa en geles; sin necesidad de calentar, reducir ni añadir un tampón de muestras antes de usarlo.

Compare y visualice todos los demás estándares y escaleras de proteínas ›

Aplicaciones
• Supervisión de la migración de proteínas durante la electroforesis en gel de poliacrilamida-SDS
• Supervisión de la transferencia de proteínas a las membranas tras Western blot
• Dimensionamiento de proteínas en geles SDS-PAGE y Western blots.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Método de detecciónColorimétrico
Compatibilidad del gelGeles Bolt™ Bis-Tris Plus, geles de tricina Novex™, geles de Tris-glicina Novex™, geles NuPAGE™ Bis-Tris
Peso molecular260, 160, 110, 80, 60, 50, 40, 30, 20, 15, 10, 3,5 kDa
Línea de productosNovex
Tipo de productoMarcadores moleculares de proteínas
Cantidad2 x 250 μl
Listo para cargarSí
Condiciones de envíoAprobado para su envío en hielo húmedo o seco
Tipo de tinciónTres colores: Rosa, amarillo, azul
Tipo de sistemaWestern Blotting, SDS-PAGE
Number of Markers12
Intervalo de tamañosDe 3,5 a 260 kDa
Unit SizeEach
Contenido y almacenamiento
Los patrones de proteínas preteñidos Novex™ Sharp se proporcionan en 2 x 250 μL (un total de 50 aplicaciones de 10 μL cada una) de mezcla estándar lista para usar. El tampón de carga estándar preteñido Novex™ Sharp consta de 65 mm de Tris pH 6,5, glicerol al 30 %, SDS al 2 % y 2,5 mm de ETDA.

Almacenar a -20 °C.

Preguntas frecuentes

Why can I not see the 3.5 kDa band of the Invitrogen Sharp Pre-Stained Standard on my NuPAGE gels?

The 3.5 kDa band is visible only on NuPAGE gels with 1X MES SDS running buffer.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What are the different molecular weight estimations for the Invitrogen Sharp Pre-Stained standard on the different Invitrogen gel types?

Invitrogen Sharp Pre-Stained Standard molecular weight estimations are the same in NuPAGE Bis-Tris, NuPAGE Tris-Acetate, Invitrogen Tris-Glycine, and Tricine Gels. The appearance/migration of the protein bands may differ depending upon the gel type and running conditions

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What are the recommended protocols for preparing Invitrogen protein standards to load on a gel?

Invitrogen protein standards do not require any additional preparation. Thaw protein standards at room temperature, vortex to mix well, and load.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

I used one of your pre-stained protein standards for a western transfer and I noticed that the intensity of the band faded from the membrane during the transfer process. Why is this?

The fading is most likely due to detergent in the western blocking/washing solutions that can remove some of the proteins from the membrane. The dye itself will not wash off of the proteins because it is covalently bound. We have found that smaller pore size membranes retain the proteins better during blocking and wash procedures, and hence recommend use of 0.2 µm instead of 0.45 µm membranes for best resolution and protein retention. After transfer, it is a good idea to circle the pre-stained bands with a pencil on the membrane, so band positions can be identified after blocking and processing.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I used one of your protein standards for a western transfer and noticed that some of the lower-molecular weight protein bands passed through the membrane. How can I resolve this issue?

- Decrease voltage, current or length of transfer time
- Make sure that the methanol concentration in the transfer buffer is proper; use a methanol concentration of 10-20% methanol removes the SDS from SDS-protein complexes and improves the binding of protein to the membrane.
- Make sure that the SDS concentration (if added) in the transfer buffer is proper, don't use more than 0.02-0.04% SDS. Using too much SDS can prevent binding of proteins to the membrane.
- Check the pore size of the membrane and the size of the target protein. Proteins smaller than 10 kDa will easily pass through a 0.45 µm pore size membrane. If proteins smaller than 10 kDa are of interest, it would be better to use a 0.2 µm pore size membrane.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all Marcadores de peso molecular de proteínas preteñidos Novex™ FAQs