PFB-FDGlu (5-(Pentafluorobenzoylamino)Fluorescein Di-β-D-Glucopyranoside)
PFB-FDGlu (5-(Pentafluorobenzoylamino)Fluorescein Di-β-D-Glucopyranoside)
Citas y referencias (16)
Invitrogen™

PFB-FDGlu (5-(Pentafluorobenzoylamino)Fluorescein Di-β-D-Glucopyranoside)

El sustrato ß-glucosidasa, 5-(pentafluorobenzoilo)fluoresceína di-ß-D-glucopiranósido (PFB-FDGlu) produce el colorante verde-fluorescente PFB-F (excitación/emisión máxima aprox. ∼492/516 nm), que parece localizar losMás información
Have Questions?
Número de catálogoCantidad
P11947
también denominado P-11947
5 mg
Número de catálogo P11947
también denominado P-11947
Precio (MXN)
-
Cantidad:
5 mg
El sustrato ß-glucosidasa,

5-(pentafluorobenzoilo)fluoresceína di-ß-D-glucopiranósido (PFB-FDGlu)
produce el colorante verde-fluorescente PFB-F (excitación/emisión máxima aprox. ∼492/516 nm), que parece localizar los compartimentos endosómico y lisosómico cuando se carga en células por pinocitosis.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Permeabilidad celularPenetra en la célula
ColorGreen
Excitación/emisión492⁄516
Para utilizar con (equipo)Citómetro de flujo
Etiqueta o tinteFluorescein
Tipo de productoSustrato
Cantidad5 mg
Condiciones de envíoTemperatura ambiente
Requisitos de almacenamientoFreezer (-5°C to -30°C)
SustratoSustrato beta-glucosidasa
Método de detecciónFluorescente
FormularioPolvo
Substrate PropertiesSustrato químico
Tipo de sustratoBeta-Glucosidase Substrate
Target EnzymeBeta-Glucosidase
Unit SizeEach
Contenido y almacenamiento
Almacenar en congelador (de -5 a -30 °C).

Citations & References (16)

Citations & References
Abstract
The Parkinson's disease risk gene cathepsin B promotes fibrillar alpha-synuclein clearance, lysosomal function and glucocerebrosidase activity in dopaminergic neurons.
Authors:Jones-Tabah J,He K,Karpilovsky N,Senkevich K,Deyab G,Pietrantonio I,Goiran T,Cousineau Y,Nikanorova D,Goldsmith T,Del Cid Pellitero E,Chen CX,Luo W,You Z,Abdian N,Ahmad J,Ruskey JA,Asayesh F,Spiegelman D,Fahn S,Waters C,Monchi O,Dauvilliers Y,Dupré N,Miliukhina I,Timofeeva A,Emelyanov A,Pchelina S,Greenbaum L,Hassin-Baer S,Alcalay RN,Milnerwood A,Durcan TM,Gan-Or Z,Fon EA
Journal:Molecular neurodegeneration
PubMed ID:39587654
Variants in the CTSB gene encoding the lysosomal hydrolase cathepsin B (catB) are associated with increased risk of Parkinson's disease (PD). However, neither the specific CTSB variants driving these associations nor the functional pathways that link catB to PD pathogenesis have been characterized. CatB activity contributes to lysosomal protein degradation ... More
Development of screening strategies for the identification of paramylon-degrading enzymes.
Authors:Gissibl A,Care A,Sun A,Hobba G,Nevalainen H,Sunna A
Journal:Journal of industrial microbiology & biotechnology
PubMed ID:30806871
Enzymatic degradation of the β-1,3-glucan paramylon could enable the production of bioactive compounds for healthcare and renewable substrates for biofuels. However, few enzymes have been found to degrade paramylon efficiently and their enzymatic mechanisms remain poorly understood. Thus, the aim of this work was to find paramylon-degrading enzymes and ways ... More
Dysregulation of mitochondria-lysosome contacts by GBA1 dysfunction in dopaminergic neuronal models of Parkinson's disease.
Authors:Kim S,Wong YC,Gao F,Krainc D
Journal:Nature communications
PubMed ID:33753743
Mitochondria-lysosome contacts are recently identified sites for mediating crosstalk between both organelles, but their role in normal and diseased human neurons remains unknown. In this study, we demonstrate that mitochondria-lysosome contacts can dynamically form in the soma, axons, and dendrites of human neurons, allowing for their bidirectional crosstalk. Parkinson's disease ... More
Flow Cytometry Measurement of Glucocerebrosidase Activity in Human Monocytes.
Authors:Hughes LP,Halliday GM,Dzamko N
Journal:Bio-protocol
PubMed ID:33659542
Glucocerebrosidase (GCase) is an important enzyme for the metabolism of glycolipids. GCase enzyme deficiency is implicated in human disease and the efficient measurement of GCase activity is important for evaluating the efficacy of therapeutics targeting this enzyme. Existing approaches to measure GCase activity include whole blood mass spectrometry-based assays, where ... More
Tau accumulation in degradative organelles is associated to lysosomal stress.
Authors:Piovesana E,Magrin C,Ciccaldo M,Sola M,Bellotto M,Molinari M,Papin S,Paganetti P
Journal:Scientific reports
PubMed ID:37865674
Neurodegenerative disorders are characterized by the brain deposition of insoluble amyloidogenic proteins, such as α-synuclein or Tau, and the concomitant deterioration of cell functions such as the autophagy-lysosomal pathway (ALP). The ALP is involved in the degradation of intracellular macromolecules including protein aggregates. ALP dysfunction due to inherited defects in ... More
View all PFB-FDGlu (5-(Pentafluorobenzoylamino)Fluorescein Di-β-D-Glucopyranoside) citations