Electroporation Cuvettes, 0.4 cm
Electroporation Cuvettes, 0.4 cm
Citas y referencias (4)
Invitrogen™

Electroporation Cuvettes, 0.4 cm

El ADN se puede introducir en las células de mamíferos, bacterias y levaduras mediante el proceso de electroporación. Invitrogen ofreceMás información
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Número de catálogoCantidad
P4605050 cubetas/bolsa
Número de catálogo P46050
Precio (MXN)
-
Cantidad:
50 cubetas/bolsa
El ADN se puede introducir en las células de mamíferos, bacterias y levaduras mediante el proceso de electroporación. Invitrogen ofrece cubetas de electroporación para su uso con células de mamíferos, bacterias o levaduras con las siguientes características prácticas:

• Compatible con prácticamente todos los dispositivos de electroporación comunes
• Irradiados con gamma y envasados individualmente para garantizar la esterilidad
• Tapón a presión con código de colores para una fácil identificación del tamaño del espacio
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Diámetro (métrico)0,4 cm
Para utilizar con (equipo)Dispositivo de electroporación
Tipo de productoCubeta de electroporación
Cantidad50 cubetas/bolsa
Condiciones de envíoTemperatura ambiente
Unit SizeEach
Contenido y almacenamiento
Cada bolsa contiene 50 cubetas estériles envasadas individualmente.

Preguntas frecuentes

Will Invitrogen cuvettes fit my electroporator?

Our cuvettes are known as the "Potter-style" cuvettes, and they fit most electroporators that accept a standard size cuvette.

Are Invitrogen electroporator cuvettes autoclavable?

No, one should not autoclave these cuvettes. They are gamma-irradiated to ensure sterility.

What electroporation conditions are recommended for transfection of EcR-293 cells?

5 µg of DNA for 1 x 10e6 cells in a 0.4 cm cuvette. Electroporator settings are routinely 500 µF and 250 Volts but this may have to be optimized depending upon the make and model of the electroporator. Following electroporation, cells are plated out onto 100 mm dishes and media is changed to selective media (400 µg/ml Zeocin) after 24 hours recovery time.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

For mammalian electroporation, do we use carrier DNA? If so, how much and what type?

Carrier DNA is unnecessary for mammalian transfection by electroporation. Invitrogen typically uses 10 µg of target DNA WITHOUT carrier DNA.

Is it possible to electroporate eukaryotic cells in 0.1 cm cuvettes?

With the exception of some fungi, 0.1 cm cuvettes are not used for electroporation of eukaryotic cells. The field strength may be set to an appropriate value but the pulse lengths will typically be too short because of the high conductance of the electroporation medium (due to the short electrode gap distance). The efficiencies of transformation are typically 0.5 - 2 logs lower. Please follow manufacturer's recommended conditions for electroporation.

Citations & References (4)

Citations & References
Abstract
Inhibitors of the proteasome block the degradation of most cell proteins and the generation of peptides presented on MHC class I molecules.
Authors: Rock K L; Gramm C; Rothstein L; Clark K; Stein R; Dick L; Hwang D; Goldberg A L;
Journal:Cell
PubMed ID:8087844
'Reagents that inhibit the ubiquitin-proteasome proteolytic pathway in cells have not been available. Peptide aldehydes that inhibit major peptidase activities of the 20S and 26S proteasomes are shown to reduce the degradation of protein and ubiquitinated protein substrates by 26S particles. Unlike inhibitors of lysosomal proteolysis, these compounds inhibit the ... More
Characterization of an iron-responsive promoter in the protozoan pathogen Trichomonas vaginalis.
Authors: Tsai Chu-Dang; Liu Hsing-Wei; Tai Jung-Hsiang;
Journal:J Biol Chem
PubMed ID:11741916
Iron has been shown to regulate transcription in the protozoan pathogen Trichomonas vaginalis. In this study, a DNA transfection system was developed to monitor ap65-1 promoter activity in response to changing iron supply. In conjunction with electrophoretic mobility shift assay, iron-induced transcription of the ap65-1 gene was shown to be ... More
Expression of human fibroblast growth factor 2 mRNA is post-transcriptionally controlled by a unique destabilizing element present in the 3'-untranslated region between alternative polyadenylation sites.
Authors: Touriol C; Morillon A; Gensac M C; Prats H; Prats A C;
Journal:J Biol Chem
PubMed ID:10409702
Fibroblast growth factor 2 (FGF-2) belongs to a family of 18 genes coding for either mitogenic differentiating factors or oncogenic proteins, the expression of which must be tightly controlled. We looked for regulatory elements in the 5823-nucleotide-long 3'-untranslated region of the FGF-2 mRNA that contains eight potential alternative polyadenylation sites. ... More
Coactivator-vitamin D receptor interactions mediate inhibition of the atrial natriuretic peptide promoter.
Authors: Chen S; Cui J; Nakamura K; Ribeiro R C; West B L; Gardner D G;
Journal:J Biol Chem
PubMed ID:10809746
We have discovered a role for coactivators binding to the AF-2 surface of the vitamin D receptor (VDR) in its negative effects on gene transcription. We tested nine amino acid residues (Ser(235), Ile(242), Lys(246), Asp(253), Ile(260), Leu(263), Leu(417), Leu(419), and Glu(420)) in human VDR which, based on homology to the ... More