Qtracker™ 655 Vascular Labels

Citas y referencias (30)

Invitrogen™

Qtracker™ 655 Vascular Labels

Los puntos cuánticos no dirigidos Qtracker™ están diseñados para ser inyectados en la vena de la cola de ratones paraMás información

Número de catálogo	Cantidad
Q21021MP	200 μl

Número de catálogo Q21021MP

Precio (MXN)

Cantidad:

200 μl

Los puntos cuánticos no dirigidos Qtracker™ están diseñados para ser inyectados en la vena de la cola de ratones para el estudio de la estructura vascular usando técnicas de obtención de imágenes de animales pequeños in vivo (SAIVI). Estos nanocristales muestran una intensa fluorescencia con emisión de desplazamiento rojo para aumentar la penetración de los tejidos, y tienen un revestimiento de superficie de PEG especialmente desarrollado para minimizar las interacciones inespecíficas y reducir la respuesta inmune del tejido. Debido a que el revestimiento de la superficie de PEG no contiene grupos funcionales reactivos, los puntos cuánticos no dirigidos Qtracker™ se conservan en circulación durante más tiempo y se pueden exponer durante hasta 3 horas con una sola inyección o durante periodos de tiempo más largos con inyecciones adicionales.

¿Necesita otro espectro de emisión o un seguimiento más prolongado? Consulte nuestros otros productos de seguimiento de células de mamífero.

Atributos principales:

• La etiqueta Qtracker™ 655 tiene Ex/Em (405-615/655) nm
• Diseñados para la adquisición de imágenes in vivo de animales pequeños
• Se introducen mediante inyección en la vena de la cola, se puede adquirir imágenes hasta 3 horas después de la inyección
• Disponibles en cuatro colores: emisión de 565 nm, 655 nm, 705 nm u 800 nm

Obtenga más información sobre SAIVI y sobre las aplicaciones de los nanocristales Qdot™.

Para uso exclusivo en investigación. No diseñado para uso terapéutico o de diagnóstico en animales o humanos.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Concentración2 μM

Tipo de coloranteNanocristales Qdot

Línea de productosQTRACKER, Qdot

Cantidad200 μl

Tipo de reactivoReactivos para imágenes vasculares

Condiciones de envíoTemperatura ambiente

TécnicaObtención de imágenes in vivo

Tipo de productoEtiqueta

Unit SizeEach

Contenido y almacenamiento

Contiene 200 μl de puntos cuánticos no dirigidos Qtracker™ (solución de 2 μM en 50 mM de tampón de borato, pH de 8,3). Almacenar de 2–6 °C. No la congele. Estable durante al menos 6 meses.

Preguntas frecuentes

When I label with Qtracker cell labeling reagents, I get a punctate label pattern. How do I make it more uniform?

Qtracker cell labeling reagents are taken up by the cell through endocytosis and sequestered in endosomes. This gives the label a punctate or vesicular appearance. This is normal. There is nothing that can be done to make it appear uniform throughout the cytoplasm.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How long after injection should I start imaging my mouse?

The imaging time course varies with the nature of the injected agent. Vascular tracers are visible in the blood vessels immediately after injection and may be imaged for several hours. Conjugated whole IgG antibodies reach their targets within a few hours of injection and may be imaged for several days.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What happens to unconjugated dye in the mouse after small animal in vivo imaging?

The dye will be eliminated via the bladder. The bladder signal is detectable within ~3 minutes of IV injection of the dye and clearance with ~30 minutes.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What amount of Qtracker non-targeted reagent should I inject to image vasculature?

A recommended starting dosage is 25-50 µL of Qtracker reagent diluted to the desired injection volume with PBS or normal saline. Qtracker reagent should be diluted immediately prior to injection. DO NOT STORE DILUTED. You will need to determine the optimal dosage for your experimental models.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to track my cells with a nucleic acid stain, like DAPI or Hoechst dye. Do you recommend this?

This is not recommended. When these stains bind to DNA and RNA, they may affect the normal function of the nucleic acids, disrupting transcription, as well as replication. Other reagents, such as CellTracker dyes or Qtracker reagents are more optimized for tracking without disrupting normal activity. If a nuclear label is still desired, though, and the cells are mammalian and non-hematopoietic, CellLight nuclear reagents can transiently transfect cells to express GFP or RFP on a nuclear-expressing protein for up to several days without affecting function.

Find additional tips, troubleshooting help, and resources within our Cell Tracing and Tracking Support Center.

View all Qtracker™ 655 Vascular Labels FAQs

Citations & References (30)

Citations & References

Abstract

Intracellular fluid flow in rapidly moving cells.

Authors:Keren K, Yam PT, Kinkhabwala A, Mogilner A, Theriot JA,

Journal:Nat Cell Biol

PubMed ID:19767741

'Cytosolic fluid dynamics have been implicated in cell motility because of the hydrodynamic forces they induce and because of their influence on transport of components of the actin machinery to the leading edge. To investigate the existence and the direction of fluid flow in rapidly moving cells, we introduced inert ... More

Circulating Bmp10 acts through endothelial Alk1 to mediate flow-dependent arterial quiescence.

Authors:Laux DW, Young S, Donovan JP, Mansfield CJ, Upton PD, Roman BL,

Journal:

PubMed ID:23863480

'Blood flow plays crucial roles in vascular development, remodeling and homeostasis, but the molecular pathways required for transducing flow signals are not well understood. In zebrafish embryos, arterial expression of activin receptor-like kinase 1 (alk1), which encodes a TGFß family type I receptor, is dependent on blood flow, and loss ... More

In vivo diffusion analysis with quantum dots and dextrans predicts the width of brain extracellular space.

Authors:Thorne RG, Nicholson C

Journal:Proc Natl Acad Sci U S A

PubMed ID:16567637

'Diffusion within the extracellular space (ECS) of the brain is necessary for chemical signaling and for neurons and glia to access nutrients and therapeutics; however, the width of the ECS in living tissue remains unknown. We used integrative optical imaging to show that dextrans and water-soluble quantum dots with Stokes-Einstein ... More

Variables influencing interactions of untargeted quantum dot nanoparticles with skin cells and identification of biochemical modulators.

Authors:Ryman-Rasmussen JP, Riviere JE, Monteiro-Riviere NA

Journal:Nano Lett

PubMed ID:17408303

Skin cells (NHEK) take up untargeted quantum dots (QD) with surface polyethylene glycol (PEG), amines, and carboxylic acids, but the mechanisms are unknown. Time courses of QD-NHEK interactions were determined and effects of QD surface coating, temperature, culture medium supplements and inhibitors of the cell cycle and endocytosis identified. The ... More

Intravital FLIM-FRET imaging reveals dasatinib-induced spatial control of src in pancreatic cancer.

Authors:Nobis M, McGhee EJ, Morton JP, Schwarz JP, Karim SA, Quinn J, Edward M, Campbell AD, McGarry LC, Evans TR, Brunton VG, Frame MC, Carragher NO, Wang Y, Sansom OJ, Timpson P, Anderson KI,

Journal:

PubMed ID:23749641

Cancer invasion and metastasis occur in a complex three-dimensional (3D) environment, with reciprocal feedback from the surrounding host tissue and vasculature-governing behavior. In this study, we used a novel intravital method that revealed spatiotemporal regulation of Src activity in response to the anti-invasive Src inhibitor dasatinib. A fluorescence lifetime imaging ... More

View all Qtracker™ 655 Vascular Labels citations