Qtracker™ 705 Vascular Labels
Qtracker™ 705 Vascular Labels
Invitrogen™

Qtracker™ 705 Vascular Labels

Los puntos cuánticos no dirigidos Qtracker™ están diseñados para ser inyectados en la vena de la cola de ratones paraMás información
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Número de catálogoCantidad
Q21061MP200 μl
Número de catálogo Q21061MP
Precio (MXN)
-
Cantidad:
200 μl
Los puntos cuánticos no dirigidos Qtracker™ están diseñados para ser inyectados en la vena de la cola de ratones para el estudio de la estructura vascular usando técnicas de obtención de imágenes de animales pequeños in vivo (SAIVI). Estos nanocristales muestran una intensa fluorescencia con emisión de desplazamiento rojo para aumentar la penetración de los tejidos, y tienen un revestimiento de superficie de PEG especialmente desarrollado para minimizar las interacciones inespecíficas y reducir la respuesta inmune del tejido. Debido a que el revestimiento de la superficie de PEG no contiene grupos funcionales reactivos, los puntos cuánticos no dirigidos Qtracker™ se conservan en circulación durante más tiempo y se pueden exponer durante hasta 3 horas con una sola inyección o durante periodos de tiempo más largos con inyecciones adicionales.

¿Necesita otro espectro de emisión o un seguimiento más prolongado? Consulte nuestros otros productos de seguimiento de células de mamífero.

Atributos principales:

La etiqueta Qtracker™ 705 tiene Ex/Em (405-665/655) nm
Diseñados para la adquisición de imágenes in vivo de animales pequeños
Se introducen mediante inyección en la vena de la cola, se puede adquirir imágenes hasta 3 horas después de la inyección
Disponibles en cuatro colores: emisión de 705 nm, 655 nm, 705 nm u 800 nm

Obtenga más información sobre SAIVI y sobre las aplicaciones de los nanocristales Qdot™.

Para uso exclusivo en investigación. No diseñado para uso terapéutico o de diagnóstico en animales o humanos.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Concentración2 μM
Tipo de coloranteNanocristales Qdot
Línea de productosQTRACKER, Qdot
Cantidad200 μl
Tipo de reactivoReactivos para imágenes vasculares
Condiciones de envíoTemperatura ambiente
TécnicaObtención de imágenes in vivo
Tipo de productoEtiqueta
Unit SizeEach
Contenido y almacenamiento
Contiene 200 μl de puntos cuánticos no dirigidos Qtracker™ (solución de 2 μM en 50 mM de tampón de borato, pH de 8,3). Almacenar de 2–6 °C. No la congele. Estable durante al menos 6 meses.

Preguntas frecuentes

When I label with Qtracker cell labeling reagents, I get a punctate label pattern. How do I make it more uniform?

Qtracker cell labeling reagents are taken up by the cell through endocytosis and sequestered in endosomes. This gives the label a punctate or vesicular appearance. This is normal. There is nothing that can be done to make it appear uniform throughout the cytoplasm.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How long after injection should I start imaging my mouse?

The imaging time course varies with the nature of the injected agent. Vascular tracers are visible in the blood vessels immediately after injection and may be imaged for several hours. Conjugated whole IgG antibodies reach their targets within a few hours of injection and may be imaged for several days.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What happens to unconjugated dye in the mouse after small animal in vivo imaging?

The dye will be eliminated via the bladder. The bladder signal is detectable within ~3 minutes of IV injection of the dye and clearance with ~30 minutes.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What amount of Qtracker non-targeted reagent should I inject to image vasculature?

A recommended starting dosage is 25-50 µL of Qtracker reagent diluted to the desired injection volume with PBS or normal saline. Qtracker reagent should be diluted immediately prior to injection. DO NOT STORE DILUTED. You will need to determine the optimal dosage for your experimental models.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to track my cells with a nucleic acid stain, like DAPI or Hoechst dye. Do you recommend this?

This is not recommended. When these stains bind to DNA and RNA, they may affect the normal function of the nucleic acids, disrupting transcription, as well as replication. Other reagents, such as CellTracker dyes or Qtracker reagents are more optimized for tracking without disrupting normal activity. If a nuclear label is still desired, though, and the cells are mammalian and non-hematopoietic, CellLight nuclear reagents can transiently transfect cells to express GFP or RFP on a nuclear-expressing protein for up to several days without affecting function.

Find additional tips, troubleshooting help, and resources within our Cell Tracing and Tracking Support Center.

Citations & References (17)

Citations & References
Abstract
Noninvasive imaging of quantum dots in mice.
Authors:Ballou B, Lagerholm BC, Ernst LA, Bruchez MP, Waggoner AS
Journal:Bioconjug Chem
PubMed ID:14733586
'Quantum dots having four different surface coatings were tested for use in in vivo imaging. Localization was successfully monitored by fluorescence imaging of living animals, by necropsy, by frozen tissue sections for optical microscopy, and by electron microscopy, on scales ranging from centimeters to nanometers, using only quantum dots for ... More
In vivo imaging of quantum dots.
Authors:Texier I, Josser V,
Journal:Methods Mol Biol
PubMed ID:19488714
'Noninvasive whole-body near-infrared fluorescence imaging is now acknowledged as a powerful method for the molecular mapping of biological events in live small animals such as mouse models. With outstanding optical properties such as high fluorescence quantum yields and low photobleaching rates, quantum dots (QDs) are labels of choice in the ... More
Imaging takes a quantum leap.
Authors:Lidke DS, Arndt-Jovin DJ
Journal:Physiology (Bethesda)
PubMed ID:15546848
Semiconducting nanocrystals, or quantum dots (QDs), have emerged as a new tool in physiological imaging, combining high brilliance, photostability, broad excitation but very narrow emission spectra, and surface chemistry compatible with biomolecular conjugation. In this review, we demonstrate the power of QDs in diverse applications, including long-term in vivo fluorescence ... More
In vivo excitation of nanoparticles using luminescent bacteria.
Authors:Dragavon J, Blazquez S, Rekiki A, Samson C, Theodorou I, Rogers KL, Tournebize R, Shorte SL,
Journal:Proc Natl Acad Sci U S A
PubMed ID:22615349
The lux operon derived from Photorhabdus luminescens incorporated into bacterial genomes, elicits the production of biological chemiluminescence typically centered on 490 nm. The light-producing bacteria are widely used for in vivo bioluminescence imaging. However, in living samples, a common difficulty is the presence of blue-green absorbers such as hemoglobin. Here we ... More
Lymph node B lymphocyte trafficking is constrained by anatomy and highly dependent upon chemoattractant desensitization.
Authors:Park C, Hwang IY, Sinha RK, Kamenyeva O, Davis MD, Kehrl JH,
Journal:Blood
PubMed ID:22039261
B lymphocyte recirculation through lymph nodes (LNs) requires crossing endothelial barriers and chemoattractant-triggered cell migration. Here we show how LN anatomy and chemoattractant receptor signaling organize B lymphocyte LN trafficking. Blood-borne B cells predominately used CCR7 signaling to adhere to high endothelial venules (HEVs). New B cell emigrants slowly transited ... More