Kits de ensayo de ADNds Quant-iT™, alta sensibilidad (HS) y amplio rango (BR)
Kits de ensayo de ADNds Quant-iT™, alta sensibilidad (HS) y amplio rango (BR)
Citas y referencias (32)
Invitrogen™

Kits de ensayo de ADNds Quant-iT™, alta sensibilidad (HS) y amplio rango (BR)

Para la cuantificación altamente selectiva de ADN bicatenario sobre ARN, los kits de ensayo de ADNbc Quant-iT, alta sensibilidad y amplio intervalo, producen señales de fluorescencia lineales en los intervalos de 0,2 a 100 ng y 2 a 1000 ng de ADN, respectivamente.
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Número de catálogoensayoIntervalo de cuantificación
Q33120Cuantificación de ADNds, alta sensibilidadDe 0,2 a 100 ng
Q33130Cuantificación de ADNds, rango amplioDe 4 a 1000 ng
Número de catálogo Q33120
Precio (MXN)
-
ensayo:
Cuantificación de ADNds, alta sensibilidad
Intervalo de cuantificación:
De 0,2 a 100 ng
Realice la cuantificación de ADNbc de forma sencilla y rápida con los kits de ensayo de ADNbc Quant-iT. Tanto el kit de ensayo de ADNbc de alta sensibilidad Quant-iT como el kit de ensayo de ADNbc de amplio rango Quant-iT proporcionan reactivo de ensayo concentrado, tampón de dilución y patrones de ADN prediluidos. Estos kits de ensayo de ADN son altamente selectivos para ADN bicatenario sobre ARN y, en los intervalos de 0,2 a 100 ng de ADN (para el kit de ensayo de ADN bicatenario de alta sensibilidad) y de 4 a 1000 ng de ADN (para el kit de ensayo de ADN bicatenario de amplio rango), la señal de fluorescencia es lineal.
El kit de ensayo de alta sensibilidad de ADNbc Quant-iT y el kit de ensayo de amplio intervalo de ADNbc Quant-iT hacen que la cuantificación del ADN sea fácil y precisa. Los kits proporcionan soluciones para el reactivo de ensayo concentrado, el tampón de dilución y el ADN prediluido. Basta con diluir el reactivo 1:200, cargar 200 µl en los pocillos de una microplaca, añadir entre 1 y 20 µl de muestra, mezclar y leer la fluorescencia.

Los ensayos son altamente selectivos para ADN bicatenario sobre ARN y, en el intervalo de 0,2 a 100 ng para el ensayo de HS o de 4 a 1000 ng para el ensayo de BR, la señal de fluorescencia es lineal con el ADN. Los ensayos se realizan a temperatura ambiente y la señal es estable durante tres horas. Estos ensayos presentan una buena tolerancia a los contaminantes comunes, como sales, disolventes, detergentes y proteínas.

For Research Use Only. Not for use in diagnostic procedures.
Especificaciones
ensayoCuantificación de ADNds, alta sensibilidad
Para utilizar con (equipo)Lector de microplacas
N.º de reacciones1000 ensayos (volumen de ensayo de 200 μl)
Línea de productosQuant-iT
Intervalo de cuantificaciónDe 0,2 a 100 ng
Cantidad1 kit
Condiciones de envíoTemperatura ambiente
Método de detecciónFluorescencia
Unit SizeEach

Preguntas frecuentes

Why am I getting negative fluorescence values with my Qubit Assays?

Negative fluorescence is a physical impossibility. It is an artifact from software autocorrecting for background signal. This means your reader is picking up and subtracting out background light at the cost of your data. Make sure to do a buffer-only control and assess the type of signal. You may need to switch to a different plate.

I have a Quant-iT DNA Kit and want to use it for the Qubit Fluorometer. Can I?

Yes, the manual has directions for this application. You will use the 0 ng/µL lambda dsDNA HS standard to generate Standard #1. You will prepare a dilution of the 10 ng/µL lambda dsDNA HS standard to generate Standard #2. You then prepare the samples and compare them to this 2-point standard curve. The Quant-iT dsDNA BR Kit can be used in a similar manner.

What is the useful pH range for Quant-iT DNA kits?

The buffer included in the kit should assure the proper pH range, even if your DNA is at a pH outside of this range, since at least a 10-fold excess of kit buffer over sample is used in the assay.

I'm trying to quantify some DNA labeled with a fluorophore. Will this work?

PicoGreen dye and other fluorescence-based quantification reagents are not recommended for quantifying dye-conjugated nucleic acids. The attached dye molecules can interfere with either binding and/or fluorescence output of the quantification reagents.

Does DNA length have an effect on the dsDNA assays?

Strands that are roughly in the 20-mer range or shorter show a lower level of signal. For dsDNA samples that are composed of mostly short strands, the reagent may still be used, but one should use a dsDNA standard that is of comparable length as the sample.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

View all Kits de ensayo de ADNds Quant-iT™, alta sensibilidad (HS) y amplio rango (BR) FAQs

Citations & References (32)

Citations & References
Abstract
Natural transformation of Myxococcus xanthus.
Authors:Wang J, Hu W, Lux R, He X, Li Y, Shi W,
Journal:J Bacteriol
PubMed ID:21378184
'Myxococcus xanthus belongs to the delta class of the proteobacteria and is notable for its complex life-style with social behaviors and relatively large genome. Although previous observations have suggested the existence of horizontal gene transfer in M. xanthus, its ability to take up exogenous DNA via natural transformation has not ... More
Gastroenteritis outbreak caused by waterborne norovirus at a New Zealand ski resort.
Authors:Hewitt J, Bell D, Simmons GC, Rivera-Aban M, Wolf S, Greening GE,
Journal:Appl Environ Microbiol
PubMed ID:17965205
'In July 2006, public health services investigated an outbreak of acute gastroenteritis among staff and visitors of a popular ski resort in southern New Zealand. The source of the outbreak was a drinking water supply contaminated by human sewage. The virological component of the investigation played a major role in ... More
Expression/localization patterns of sirtuins (SIRT1, SIRT2, and SIRT7) during progression of cervical cancer and effects of sirtuin inhibitors on growth of cervical cancer cells.
Authors:Singh S, Kumar PU, Thakur S, Kiran S, Sen B, Sharma S, Rao VV, Poongothai AR, Ramakrishna G
Journal:
PubMed ID:25794641
'Sirtuins belong to the family of class III histone deacetylases; its role in neoplasia is controversial as both tumor-suppressive and promoting functions have been reported. There are very few reports available, where expressions of sirtuin isoforms are comprehensively analyzed during neoplasia. Therefore, in the present study, the expression of SIRT1, ... More
The altered landscape of the human skin microbiome in patients with primary immunodeficiencies.
Authors:Oh J, Freeman AF, Park M, Sokolic R, Candotti F, Holland SM, Segre JA, Kong HH,
Journal:
PubMed ID:24170601
'While landmark studies have shown that microbiota activate and educate host immunity, how immune systems shape microbiomes and contribute to disease is incompletely characterized. Primary immunodeficiency (PID) patients suffer recurrent microbial infections, providing a unique opportunity to address this issue. To investigate the potential influence of host immunity on the ... More
Effect of the metabolic environment at key stages of follicle development in cattle: focus on steroid biosynthesis.
Authors:Walsh SW, Mehta JP, McGettigan PA, Browne JA, Forde N, Alibrahim RM, Mulligan FJ, Loftus B, Crowe MA, Matthews D, Diskin M, Mihm M, Evans AC,
Journal:Physiol Genomics
PubMed ID:22414914
'Cellular mechanisms that contribute to low estradiol concentrations produced by the preovulatory ovarian follicle in cattle with a compromised metabolic status are largely unknown. To gain insight into the main metabolic mechanisms affecting preovulatory follicle function, two different animal models were used. Experiment 1 compared Holstein-Friesian nonlactating heifers (n = ... More
View all Kits de ensayo de ADNds Quant-iT™, alta sensibilidad (HS) y amplio rango (BR) citations