Kits de ensayo de ADNds Quant-iT™, alta sensibilidad (HS) y amplio rango (BR)
Kits de ensayo de ADNds Quant-iT™, alta sensibilidad (HS) y amplio rango (BR)
Invitrogen™

Kits de ensayo de ADNds Quant-iT™, alta sensibilidad (HS) y amplio rango (BR)

Para la cuantificación altamente selectiva de ADN bicatenario sobre ARN, los kits de ensayo de ADNbc Quant-iT, alta sensibilidad y amplio intervalo, producen señales de fluorescencia lineales en los intervalos de 0,2 a 100 ng y 2 a 1000 ng de ADN, respectivamente.
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Número de catálogoensayoIntervalo de cuantificación
Q33130Cuantificación de ADNds, rango amplioDe 4 a 1000 ng
Q33120Cuantificación de ADNds, alta sensibilidadDe 0,2 a 100 ng
Número de catálogo Q33130
Precio (MXN)
-
ensayo:
Cuantificación de ADNds, rango amplio
Intervalo de cuantificación:
De 4 a 1000 ng
Realice la cuantificación de ADNbc de forma sencilla y rápida con los kits de ensayo de ADNbc Quant-iT. Tanto el kit de ensayo de ADNbc de alta sensibilidad Quant-iT como el kit de ensayo de ADNbc de amplio rango Quant-iT proporcionan reactivo de ensayo concentrado, tampón de dilución y patrones de ADN prediluidos. Estos kits de ensayo de ADN son altamente selectivos para ADN bicatenario sobre ARN y, en los intervalos de 0,2 a 100 ng de ADN (para el kit de ensayo de ADN bicatenario de alta sensibilidad) y de 4 a 1000 ng de ADN (para el kit de ensayo de ADN bicatenario de amplio rango), la señal de fluorescencia es lineal.
El kit de ensayo de alta sensibilidad de ADNbc Quant-iT y el kit de ensayo de amplio intervalo de ADNbc Quant-iT hacen que la cuantificación del ADN sea fácil y precisa. Los kits proporcionan soluciones para el reactivo de ensayo concentrado, el tampón de dilución y el ADN prediluido. Basta con diluir el reactivo 1:200, cargar 200 µl en los pocillos de una microplaca, añadir entre 1 y 20 µl de muestra, mezclar y leer la fluorescencia.

Los ensayos son altamente selectivos para ADN bicatenario sobre ARN y, en el intervalo de 0,2 a 100 ng para el ensayo de HS o de 4 a 1000 ng para el ensayo de BR, la señal de fluorescencia es lineal con el ADN. Los ensayos se realizan a temperatura ambiente y la señal es estable durante tres horas. Estos ensayos presentan una buena tolerancia a los contaminantes comunes, como sales, disolventes, detergentes y proteínas.

For Research Use Only. Not for use in diagnostic procedures.
Especificaciones
ensayoCuantificación de ADNds, rango amplio
Para utilizar con (equipo)Lector de microplacas
N.º de reacciones1000 ensayos (volumen de ensayo de 200 μl)
Línea de productosQuant-iT
Intervalo de cuantificaciónDe 4 a 1000 ng
Cantidad1 kit
Condiciones de envíoTemperatura ambiente
Método de detecciónFluorescencia
Unit SizeEach

Preguntas frecuentes

Why am I getting negative fluorescence values with my Qubit Assays?

Negative fluorescence is a physical impossibility. It is an artifact from software autocorrecting for background signal. This means your reader is picking up and subtracting out background light at the cost of your data. Make sure to do a buffer-only control and assess the type of signal. You may need to switch to a different plate.

I have a Quant-iT DNA Kit and want to use it for the Qubit Fluorometer. Can I?

Yes, the manual has directions for this application. You will use the 0 ng/µL lambda dsDNA HS standard to generate Standard #1. You will prepare a dilution of the 10 ng/µL lambda dsDNA HS standard to generate Standard #2. You then prepare the samples and compare them to this 2-point standard curve. The Quant-iT dsDNA BR Kit can be used in a similar manner.

What is the useful pH range for Quant-iT DNA kits?

The buffer included in the kit should assure the proper pH range, even if your DNA is at a pH outside of this range, since at least a 10-fold excess of kit buffer over sample is used in the assay.

I'm trying to quantify some DNA labeled with a fluorophore. Will this work?

PicoGreen dye and other fluorescence-based quantification reagents are not recommended for quantifying dye-conjugated nucleic acids. The attached dye molecules can interfere with either binding and/or fluorescence output of the quantification reagents.

Does DNA length have an effect on the dsDNA assays?

Strands that are roughly in the 20-mer range or shorter show a lower level of signal. For dsDNA samples that are composed of mostly short strands, the reagent may still be used, but one should use a dsDNA standard that is of comparable length as the sample.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

Citations & References (16)

Citations & References
Abstract
3D chromatin conformation correlates with replication timing and is conserved in resting cells.
Authors:Moindrot B, Audit B, Klous P, Baker A, Thermes C, de Laat W, Bouvet P, Mongelard F, Arneodo A,
Journal:Nucleic Acids Res
PubMed ID:22879376
'Although chromatin folding is known to be of functional importance to control the gene expression program, less is known regarding its interplay with DNA replication. Here, using Circular Chromatin Conformation Capture combined with high-throughput sequencing, we identified megabase-sized self-interacting domains in the nucleus of a human lymphoblastoid cell line, as ... More
A significant effect of the TSPY1 copy number on spermatogenesis efficiency and the phenotypic expression of the gr/gr deletion.
Authors:Shen Y, Yan Y, Liu Y, Zhang S, Yang D, Zhang P, Li L, Wang Y, Ma Y, Tao D, Yang Y,
Journal:Hum Mol Genet
PubMed ID:23307928
'AZFc deletions cause a significant phenotypic heterogeneity with respect to spermatogenesis; however, the reason for this is poorly understood. Recently, testis-specific protein Y-encoded 1 (TSPY1) copy number variation (CNV) was determined to be a potential genetic modifier of spermatogenesis. We performed a large-scale cohort study to investigate the effect of ... More
Gene expression profiling in insulinomas of Men1 beta-cell mutant mice reveals early genetic and epigenetic events involved in pancreatic beta-cell tumorigenesis.
Authors:Fontanière S, Tost J, Wierinckx A, Lachuer J, Lu J, Hussein N, Busato F, Gut I, Wang ZQ, Zhang CX,
Journal:Endocr Relat Cancer
PubMed ID:17158767
'Mutations of the MEN1 gene lead to the occurrence of multiple endocrine neoplasia type 1 (MEN1). To gain insights into the mechanisms of the tumorigenesis related to MEN1 inactivation, we have used mice in which the Men1 gene was specifically disrupted in pancreatic beta-cells. In these mice, we observed full ... More
Effects of essential oils on methane production and fermentation by, and abundance and diversity of, rumen microbial populations.
Authors:Patra AK, Yu Z,
Journal:Appl Environ Microbiol
PubMed ID:22492451
'Five essential oils (EOs), namely, clove oil (CLO), eucalyptus oil (EUO), garlic oil (GAO), origanum oil (ORO), and peppermint oil (PEO), were tested in vitro at 3 different doses (0.25, 0.50, and 1.0 g/liter) for their effect on methane production, fermentation, and select groups of ruminal microbes, including total bacteria, ... More
Normal variants of Microcephalin and ASPM do not account for brain size variability.
Authors:Woods RP, Freimer NB, De Young JA, Fears SC, Sicotte NL, Service SK, Valentino DJ, Toga AW, Mazziotta JC,
Journal:Hum Mol Genet
PubMed ID:16687438
'Normal human brain volume is heritable. The genes responsible for variation in brain volume are not known. Microcephalin (MCPH1) and ASPM (abnormal spindle-like microcephaly associated) have been proposed as candidate genes as mutations in both genes are associated with microcephaly, and common variants of each gene are apparently under strong ... More