dNTP/dUTP Mix

Thermo Scientific™ dNTP/dUTP Mix is a solution containing dATP, dCTP and dGTP, each at a final concentration of 2 mM and dUTP of 4 mM. All nucleotides have greater than 99% purity and are free of nuclease activities and human and E. coli DNA. The mix offers the possibility to reduce the number of pipetting steps and the risk of reaction set up errors. dNTP/dUTP Mix is designed for direct use in PCR with Taq DNA Polymerase, reverse transcription, and a wide variety of molecular applications.

Features

• Greater than 99% purity confirmed by HPLC
• Free of human and E. coli DNA

High stability

• Stable for a minimum 100 freeze-thaw cycles
• Up to 95% of dNTPs remain in triphosphate form even after 7 weeks at room temperature
• Up to 90% of dNTPs remain in triphosphate form after 30 cycles of PCR (1 min at 94°C; 3 min at 72°C)

Applications

• cDNA synthesis and RT-PCR
• PCR, qPCR
• Standard PCR
• DNA Sequencing

Note: dNTP/dUTP Mix is not suitable for PCR with proofreading enzymes or enzyme mixes containing proofreading enzymes.