LAP Disk

Detect leucine aminopeptidase activity in streptococci and related genera with Thermo Scientific™ Remel™ LAP Disk.

Collman and Ball investigated the use of leucine aminopeptidase (LAP) that helped to identify Gram-positive cocci¹. Further, Fertally and Facklam² used LAP to identify Streptococcus spp. They found that Aerococcus is a consistent negative strain for LAP whereas Enterococcus and Streptococcus are positive strains. LAP also helps in identification of Leuconostoc, Pediococcus, Aerococcus and related genera³.

Activity of the enzyme leucine aminopeptidase can help in identifying streptococci and related genera using the LAP Disk.

• Ready to use: Reagent-impregnated disk. No further preparation necessary.
• Easy to read: Visible color change indicates positive and negative test.
  Positive test: Color change from pink to red.
  Negative test: No color change.

The LAP Disk contains L-leucine-β-naphthylamide as substrate that is hydrolyzed by leucine aminopeptidase and releases β-naphthylamide. A positive test is indicated when the color of the disk changes from white to pink to red when ρ-dimethyl-aminocinnamaldehyde (PYR Reagent) is added.

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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.