SYTO™ 61 Red Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO

Citas y referencias (17)

Invitrogen™

SYTO™ 61 Red Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO

La tinción de ácido nucleico rojo fluorescente permeable en células SYTO 61 exhibe fluorescencia rojo brillante al enlazarse con losMás información

Número de catálogo	Cantidad
S11343	250 μL

Número de catálogo S11343

Precio (MXN)

Cantidad:

250 μL

La tinción de ácido nucleico rojo fluorescente permeable en células SYTO 61 exhibe fluorescencia rojo brillante al enlazarse con los ácidos nucleicos. Debido a que el patrón de tinción de SYTO en las células vivas puede variar entre los diferentes tipos de células, ofrecemos el kit de muestreo para tinción de ácido nucleico rojo fluorescente SYTO (S-11340) para permitir a los investigadores encontrar el tinte más adecuado para su sistema.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

ColorRojo

DescripciónTinción de ácido nucleico SYTO™ 61 Red Fluorescent - Solución de 5 mm en DMSO

Método de detecciónFluorescente

Tipo de colorantePermeabilidad celular

Emisión645 nm

Intervalo de longitud de onda de excitación628 nm

Para utilizar con (equipo)Microscopio de fluorescencia

Línea de productosSYTO

Cantidad250 μL

Condiciones de envíoTemperatura ambiente

Volumen (métrico)250 μl

Tipo de etiquetaFluorescente

Tipo de productoTinción de ácidos nucleicos

SubCellular LocalizationÁcidos nucleicos

Unit SizeEach

Contenido y almacenamiento

Almacenar en el congelador de -5 °C a -30 °C y proteger de la luz.

Preguntas frecuentes

How do SYTO dyes bind to DNA?

The binding mode of SYTO nucleic acid stains is unknown. However, the behavior of these and related nucleic acid dyes suggests the following binding properties:

1.They appear to contact the solvent (suggested by sensitivity to salt, divalent cations, and in particular, SDS) and thus are likely to have contacts in the grooves.
2.All SYTO dyes appear to show some base selectivity and are thus likely to have minor groove contacts.
3.They can be removed from nucleic acid via ethanol precipitation; this characteristic is not shared by ethidium bromide and other intercalators. Likewise, the dyes are not removed from nucleic acid via butanol or chloroform extraction. These extraction methods do remove ethidium bromide from nucleic acid. 4. SYTO binding is not affected by nonionic detergents.
5. SYTO dyes are not quenched by BrdU, so they do not bind nucleic acids in precisely the same way as Hoechst 33342 and DAPI ((4′,6-diamidino-2-phenylindole).

SYBR Green I has shown little mutagenicity on frameshift indicator strains, indicating that it isn't likely to strongly intercalate.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (17)

Citations & References

Abstract

Multiparameter detection of apoptosis using red-excitable SYTO probes.

Authors:Wlodkowic D, Skommer J, Hillier C, Darzynkiewicz Z,

Journal:Cytometry A

PubMed ID:18431792

'Functional assays allowing phenotypic characterization of different cell death parameters at a single-cell level are important tools for preclinical anticancer drug screening. Currently, the selection of cytometric assays is limited by the availability of fluorescent probes with overlapping spectral characteristics. Following on our earlier reports on green and orange fluorescent ... More

Modulation of [Ca2+]i signaling dynamics and metabolism by perinuclear mitochondria in mouse parotid acinar cells.

Authors:Bruce JI, Giovannucci DR, Blinder G, Shuttleworth TJ, Yule DI

Journal:J Biol Chem

PubMed ID:14699167

'Parotid acinar cells exhibit rapid cytosolic calcium signals ([Ca2+]i) that initiate in the apical region but rapidly become global in nature. These characteristic [Ca2+]i signals are important for effective fluid secretion, which critically depends on a synchronized activation of spatially separated ion fluxes. Apically restricted [Ca2+]i signals were never observed ... More

An evaluation of the performance of cDNA microarrays for detecting changes in global mRNA expression.

Authors:Yue H, Eastman PS, Wang BB, Minor J, Doctolero MH, Nuttall RL, Stack R, Becker JW, Montgomery JR, Vainer M, Johnston R

Journal:Nucleic Acids Res

PubMed ID:11292855

'The cDNA microarray is one technological approach that has the potential to accurately measure changes in global mRNA expression levels. We report an assessment of an optimized cDNA microarray platform to generate accurate, precise and reliable data consistent with the objective of using microarrays as an acquisition platform to populate ... More

Differentiation of Phytophthora infestans sporangia from other airborne biological particles by flow cytometry.

Authors:Day JP, Kell DB, Griffith GW

Journal:Appl Environ Microbiol

PubMed ID:11772606

'The ability of two different flow cytometers, the Microcyte (Optoflow) and the PAS-III (Partec), to differentiate sporangia of the late-blight pathogen Phytophthora infestans from other potential airborne particles was compared. With the PAS-III, light scatter and intrinsic fluorescence parameters could be used to differentiate sporangia from conidia of Alternaria or ... More

The use of fluorescence enhancement to improve the microscopic diagnosis of falciparum malaria.

Authors:Guy R, Liu P, Pennefather P, Crandall I,

Journal:Malar J

PubMed ID:17617912

'BACKGROUND: Giemsa staining of thick blood smears remains the "gold standard" for detecting malaria. However, this method is not very good for diagnosing low-level infections. A method for the simultaneous staining of Plasmodium-parasitized culture and blood smears for both bright field and fluorescence was developed and its ability to improve ... More

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