SAIVI™ Rapid Antibody Labeling Kit, Alexa Fluor™ 680

Citas y referencias (5)

Invitrogen™

SAIVI™ Rapid Antibody Labeling Kit, Alexa Fluor™ 680

El kit de etiquetado de anticuerpos Alexa Fluor™ 680 SAIVI™ proporciona un medio práctico para marcar anticuerpos con un gradoMás información

Número de catálogo	Cantidad
S30045	1 kit

Número de catálogo S30045

Precio (MXN)

Cantidad:

1 kit

El kit de etiquetado de anticuerpos Alexa Fluor™ 680 SAIVI™ proporciona un medio práctico para marcar anticuerpos con un grado óptimo de marcación para aplicaciones de adquisición de imágenes in vivo (DOL; ∼2) en un rango de concentración de anticuerpos multiplicado por seis sin necesidad de ajustes en el volumen de reacción, la concentración de colorante o la concentración de anticuerpos. Mediante este procedimiento, los anticuerpos etiquetados de forma óptima están listos para las aplicaciones que requieren reactivos sin azida, como las imágenes de células vivas o la inyección directa en animales.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Colorinfrarrojo

Método de detecciónFluorescencia

Excitación/emisión679/702

Tipo de etiquetaColorantes Alexa Fluor

Escala de etiquetadoDe 0,5 a 3 mg

Línea de productosAlexa Fluor, SAIVI

Tipo de productoKit de etiquetado de anticuerpos

Cantidad1 kit

Condiciones de envíoTemperatura ambiente

Labeling TargetAnticuerpos

Etiqueta o tinteAlexa Fluor 680

Unit SizeEach

Contenido y almacenamiento

Almacenar en el refrigerador (de 2 °C a 8 °C) y proteger de la luz.

Preguntas frecuentes

What amount of conjugated antibody should I inject to image tumors?

A recommended starting dosage is 50 µg. You will need to determine the optimal dosage for your experimental model.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What size needle should I use for small animal in vivo imaging?

We recommend the use of a 28-32 gauge tuberculin or insulin syringe (0.3 or 1.0 mL volume) with a fixed (non-removable) needle.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What are the optimal and maximal volumes of reagent that can be injected into a mouse?

The volume of reagent that can be injected varies according to the route of administration. The following numbers are general guidelines for a 25 gram animal: Intravenous (IV)- 50-125 µl (recommended)- 200µl (maximum) ; Intraperitoneal (IP) 500µl (recommended) -2ml (maximum) ; Subcutaneous(SC) 100-250 µl (recommended)- 1ml (maximum).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What type of mice are the best for in vivo imaging?

Due to light scattering caused by fur, hairless mice such as athymic nude (nu/nu) mice are recommended for in vivo imaging. If this is not an option, the hair covering the area to be imaged should be removed using clippers or a chemical depilatory such as Nair depilatory.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (5)

Citations & References

Abstract

Affibody Molecules for In vivo Characterization of HER2-Positive Tumors by Near-Infrared Imaging.

Authors:Lee SB, Hassan M, Fisher R, Chertov O, Chernomordik V, Kramer-Marek G, Gandjbakhche A, Capala J,

Journal:Clin Cancer Res

PubMed ID:18559604

'PURPOSE: HER2 overexpression has been associated with a poor prognosis and resistance to therapy in breast cancer patients. We are developing molecular probes for in vivo quantitative imaging of HER2 receptors using near-infrared (NIR) optical imaging. The goal is to provide probes that will minimally interfere with the studied system, ... More

Noninvasive positron emission tomography and fluorescence imaging of CD133+ tumor stem cells.

Authors:Gaedicke S, Braun F, Prasad S, Machein M, Firat E, Hettich M, Gudihal R, Zhu X, Klingner K, Schüler J, Herold-Mende CC, Grosu AL, Behe M, Weber W, Mäcke H, Niedermann G,

Journal:

PubMed ID:24469819

'A technology that visualizes tumor stem cells with clinically relevant tracers could have a broad impact on cancer diagnosis and treatment. The AC133 epitope of CD133 currently is one of the best-characterized tumor stem cell markers for many intra- and extracranial tumor entities. Here we demonstrate the successful noninvasive detection ... More

Minimally invasive quantification of lymph flow in mice and rats by imaging depot clearance of near-infrared albumin.

Authors:Karlsen TV, McCormack E, Mujic M, Tenstad O, Wiig H,

Journal:Am J Physiol Heart Circ Physiol

PubMed ID:22101523

'There is a lack of available methods to noninvasively quantify lymphatic function in small experimental animals, a necessity for studies on lymphatic system pathophysiology. We present a new method to quantify lymph flow in mice and rats, based on optically monitoring the depot clearance of near-infrared fluorescently labeled albumin and ... More

Multiplexed mAbs: a new strategy in preclinical time-domain imaging of acute myeloid leukemia.

Authors:McCormack E, Mujic M, Osdal T, Bruserud Ø, Gjertsen BT,

Journal:Blood

PubMed ID:23243270

Antibodies play a fundamental role in diagnostic immunophenotyping of leukemias and in cell-targeting therapy. However, this versatility is not reflected in imaging diagnostics. In the present study, we labeled anti–human mAbs monochromatically against selected human myeloid markers expressed on acute myeloid leukemia (AML) cells, all with the same near-infrared fluorochrome. ... More

Influenza virus aerosol exposure and analytical system for ferrets.

Authors:Gustin KM, Belser JA, Wadford DA, Pearce MB, Katz JM, Tumpey TM, Maines TR,

Journal:Proc Natl Acad Sci U S A

PubMed ID:21536880

Understanding the transmission ability of newly emerging influenza viruses is central to the development of public health preparedness and prevention strategies. Animals are used to model influenza virus infection and transmission, but the routinely used intranasal inoculation of a liquid virus suspension does not reflect natural infection. We report the ... More