SAIVI™ Rapid Antibody Labeling Kit, Alexa Fluor™ 750
SAIVI™ Rapid Antibody Labeling Kit, Alexa Fluor™ 750
Citas y referencias (3)
Invitrogen™

SAIVI™ Rapid Antibody Labeling Kit, Alexa Fluor™ 750

El kit de etiquetado de anticuerpos Alexa Fluor™ 750 SAIVI™ proporciona un medio práctico para marcar anticuerpos con un gradoMás información
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Número de catálogoCantidad
S300461 kit
Número de catálogo S30046
Precio (MXN)
-
Cantidad:
1 kit
El kit de etiquetado de anticuerpos Alexa Fluor™ 750 SAIVI™ proporciona un medio práctico para marcar anticuerpos con un grado óptimo de marcación para aplicaciones de adquisición de imágenes in vivo (DOL; ∼2) en un rango de concentración de anticuerpos multiplicado por seis sin necesidad de ajustes en el volumen de reacción, la concentración de colorante o la concentración de anticuerpos. Mediante este procedimiento, los anticuerpos etiquetados de forma óptima están listos para las aplicaciones que requieren reactivos sin azida, como las imágenes de células vivas o la inyección directa en animales.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Colorinfrarrojo
Método de detecciónFluorescencia
Excitación/emisión749/775
Tipo de etiquetaColorantes Alexa Fluor
Escala de etiquetadoDe 0,5 a 3 mg
Línea de productosAlexa Fluor, SAIVI
Tipo de productoKit de etiquetado de anticuerpos
Cantidad1 kit
Condiciones de envíoTemperatura ambiente
Labeling TargetAnticuerpos
Etiqueta o tinteAlexa Fluor 750
Unit SizeEach
Contenido y almacenamiento
Almacenar en el refrigerador (de 2 °C a 8 °C) y proteger de la luz.

Preguntas frecuentes

What amount of conjugated antibody should I inject to image tumors?

A recommended starting dosage is 50 µg. You will need to determine the optimal dosage for your experimental model.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What size needle should I use for small animal in vivo imaging?

We recommend the use of a 28-32 gauge tuberculin or insulin syringe (0.3 or 1.0 mL volume) with a fixed (non-removable) needle.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What are the optimal and maximal volumes of reagent that can be injected into a mouse?

The volume of reagent that can be injected varies according to the route of administration. The following numbers are general guidelines for a 25 gram animal: Intravenous (IV)- 50-125 µl (recommended)- 200µl (maximum) ; Intraperitoneal (IP) 500µl (recommended) -2ml (maximum) ; Subcutaneous(SC) 100-250 µl (recommended)- 1ml (maximum).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What type of mice are the best for in vivo imaging?

Due to light scattering caused by fur, hairless mice such as athymic nude (nu/nu) mice are recommended for in vivo imaging. If this is not an option, the hair covering the area to be imaged should be removed using clippers or a chemical depilatory such as Nair depilatory.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (3)

Citations & References
Abstract
Affibody Molecules for In vivo Characterization of HER2-Positive Tumors by Near-Infrared Imaging.
Authors:Lee SB, Hassan M, Fisher R, Chertov O, Chernomordik V, Kramer-Marek G, Gandjbakhche A, Capala J,
Journal:Clin Cancer Res
PubMed ID:18559604
'PURPOSE: HER2 overexpression has been associated with a poor prognosis and resistance to therapy in breast cancer patients. We are developing molecular probes for in vivo quantitative imaging of HER2 receptors using near-infrared (NIR) optical imaging. The goal is to provide probes that will minimally interfere with the studied system, ... More
A leukocyte-mimetic magnetic resonance imaging contrast agent homes rapidly to activated endothelium and tracks with atherosclerotic lesion macrophage content.
Authors:McAteer MA, Mankia K, Ruparelia N, Jefferson A, Nugent HB, Stork LA, Channon KM, Schneider JE, Choudhury RP,
Journal:Arterioscler Thromb Vasc Biol
PubMed ID:22499989
Endothelial cell activation is an important mediator of monocyte recruitment to sites of vascular inflammation. We hypothesized that high-affinity dual-ligand microparticles of iron oxide (MPIO), targeted to P-selectin and vascular cell adhesion molecule-1 (PV-MPIO), would identify activated endothelial cells during atherosclerosis progression. In vivo magnetic resonance imaging in apolipoprotein E-deficient ... More
Blocking ephrinB2 with highly specific antibodies inhibits angiogenesis, lymphangiogenesis, and tumor growth.
Authors:Abéngozar MA, de Frutos S, Ferreiro S, Soriano J, Perez-Martinez M, Olmeda D, Marenchino M, Cañamero M, Ortega S, Megias D, Rodriguez A, Martínez-Torrecuadrada JL,
Journal:Blood
PubMed ID:22446484
Membrane-anchored ephrinB2 and its receptor EphB4 are involved in the formation of blood and lymphatic vessels in normal and pathologic conditions. Eph/ephrin activation requires cell-cell interactions and leads to bidirectional signaling pathways in both ligand- and receptor-expressing cells. To investigate the functional consequences of blocking ephrinB2 activity, 2 highly specific ... More