Nuestro conjugado de estreptavidina Alexa Fluor 405 es una sonda brillante y fotoestable con características de excitación/emisión máximas de 402Más información
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Número de catálogo
Cantidad
S32351
1 mg
Número de catálogo S32351
Precio (MXN)
-
Cantidad:
1 mg
Nuestro conjugado de estreptavidina Alexa Fluor 405 es una sonda brillante y fotoestable con características de excitación/emisión máximas de 402 y 421 nm. La estreptavidina es una proteína no glucosilada de unión de biotina que se aísla de bacterias y tiene un punto isoeléctrico casi neutro. Se utiliza ampliamente para detectar sondas biotiniladas, porque se ha constatado que exhibe una unión menos inespecífica que la avidina, una proteína glucosilada de unión de biotina.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Método de detecciónFluorescente
Excitación/emisión402/421
Líneas láser del citómetro de flujo405
Para utilizar con (equipo)Citometría de flujo, inmunoensayos, aplicaciones histoquímicas, análisis de blot
Etiqueta o tinteAlexa Fluor 405
Tipo de productoConjugado de estreptavidina (Fluorescente)
Cantidad1 mg
Condiciones de envíoTemperatura ambiente
ConjugadoAlexa Fluor 405
FormularioSólido
Línea de productosAlexa Fluor
Unit SizeEach
Contenido y almacenamiento
Contiene un vial de estreptavidina, conjugado Alexa Fluor™ 405.
Almacenar en el congelador (de – 5 a – 30 °C) y proteger de la luz.
Preguntas frecuentes
I am planning to use a fluorescent streptavidin labeled conjugate. What are the storage conditions and shelf life for the lyophilized powder and reconstituted solution?
In the lyophilized powder form, the fluorescent streptavidin labeled conjugate is stable for six months when stored at -20 degrees C, desiccated, and protected from light. The reconstituted solution is stable for approximately six months when stored at 4 degrees C, protected from light, with the addition of sodium azide to a final concentration of 5 mM or thimerosal to 0.2 mM. For longer storage, we recommend dividing the solution into aliquots and freezing at -20 degrees C, protected from light. Avoid repeated freezing and thawing of the solution.
I am planning to use a fluorescent streptavidin labeled conjugate. How should I prepare the working solution of the conjugate?
The fluorescent streptavidin labeled conjugate solution can be made by dissolving the powder in 0.5-1.0 mL of PBS or other suitable buffer. For details, please refer to page 4 of the "Streptavidin and Fluorescent Conjugates of Streptavidin" manual (https://assets.fishersci.com/TFS-Assets/LSG/manuals/mp00888.pdf).
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