SelectFX™ Nuclear Labeling Kit (DAPI, SYTOX™ Green, 7-AAD, TO-PRO™-3 Iodide), for fixed cells
SelectFX™ Nuclear Labeling Kit (DAPI, SYTOX™ Green, 7-AAD, TO-PRO™-3 Iodide), for fixed cells
Citas y referencias (14)
Invitrogen™

SelectFX™ Nuclear Labeling Kit (DAPI, SYTOX™ Green, 7-AAD, TO-PRO™-3 Iodide), for fixed cells

El kit de etiquetado nuclear SelectFX™ proporciona cuatro colorantes fluorescentes espectralmente distintos para preparación de células fijas: azul-fluorescente DAPI, tinciónMás información
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Número de catálogoCantidad
S330251 kit
Número de catálogo S33025
Precio (MXN)
-
Cantidad:
1 kit
El kit de etiquetado nuclear SelectFX™ proporciona cuatro colorantes fluorescentes espectralmente distintos para preparación de células fijas: azul-fluorescente DAPI, tinción verde-fluorescente SYTOX™, aminoactinomicina D rojo fluorescente 7 (7-AAD) y rojo-fluorescente lejano TO-PRO™-3. Estos colorantes son ideales para su uso como contratinción en aplicaciones multicolores; solo tiene que seleccionar la tinción que contraste espectralmente con otras sondas fluorescentes aplicadas a la muestra. Cuando se utilizan de acuerdo con el protocolo proporcionado, los colorantes del kit de etiquetado nuclear SelectFX™ proporcionan una tinción nuclear altamente selectiva con poco o ningún etiquetado citoplasmático.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
ColorVerde
Método de detecciónFluorescente
Tipo de coloranteImpenetrable en células
Para utilizar con (equipo)Microscopio de fluorescencia, citómetro de flujo
Línea de productosSELECTFX, SYTOX, TO-PRO
Cantidad1 kit
Condiciones de envíoTemperatura ambiente
Tipo de etiquetaFluorescent Dye
Tipo de productoKit de etiquetado nuclear
SubCellular LocalizationNucleicos, ácidos nucleicos, Nucleus
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador de -5 °C a -30 °C y proteger de la luz.

Preguntas frecuentes

How do SYTO dyes bind to DNA?

The binding mode of SYTO nucleic acid stains is unknown. However, the behavior of these and related nucleic acid dyes suggests the following binding properties:

1.They appear to contact the solvent (suggested by sensitivity to salt, divalent cations, and in particular, SDS) and thus are likely to have contacts in the grooves.
2.All SYTO dyes appear to show some base selectivity and are thus likely to have minor groove contacts.
3.They can be removed from nucleic acid via ethanol precipitation; this characteristic is not shared by ethidium bromide and other intercalators. Likewise, the dyes are not removed from nucleic acid via butanol or chloroform extraction. These extraction methods do remove ethidium bromide from nucleic acid. 4. SYTO binding is not affected by nonionic detergents.
5. SYTO dyes are not quenched by BrdU, so they do not bind nucleic acids in precisely the same way as Hoechst 33342 and DAPI ((4′,6-diamidino-2-phenylindole).

SYBR Green I has shown little mutagenicity on frameshift indicator strains, indicating that it isn't likely to strongly intercalate.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

My DAPI labeled samples have a strong blue background signal immediately after mounting. What can I do to fix this?

Some mounting medias can have strong blue autofluorescence. If you are seeing a high blue background, it could be coming from the mountant. Try labeling the sample and view it before (using a wet mount in buffer) and after mounting to determine if the background signal is coming from the mounting media or the sample itself.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am using SYTOX AAdvanced as a dead cell stain, but all of my cells are labeling even though I am certain that they are supposed to be alive. These are adherent cells that I have trypsinized. Why am I getting false-dead signals?

SYTOX AAdvanced labels only dead cells because it is a cell impermeant dye. The dye can only enter cells that have a compromised plasma membrane. Trypsinization may cause temporary disruption of the plasma membrane, sufficient to allow staining with a cell impermeant dye. You can reduce the “false-dead” problem by either reducing the amount of trypsin and/or reduce the incubation time for trypsinization or use a gentler dissociation reagent such as TrypLE Express, TrypLESelect reagents, or Versene. After trypsinization, wash well, and if possible, allow a recovery time in normal culture media before staining with any of the SYTOX dyes.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (14)

Citations & References
Abstract
Avoiding cytotoxicity of transposases by dose-controlled mRNA delivery.
Authors:Galla M, Schambach A, Falk CS, Maetzig T, Kuehle J, Lange K, Zychlinski D, Heinz N, Brugman MH, Göhring G, Izsvák Z, Ivics Z, Baum C,
Journal:Nucleic Acids Res
PubMed ID:21609958
'The Sleeping Beauty (SB) transposase and its newly developed hyperactive variant, SB100X, are of increasing interest for genome modification in experimental models and gene therapy. The potential cytotoxicity of transposases requires careful assessment, considering that residual integration events of transposase expression vectors delivered by physicochemical transfection or episomal retroviral vectors ... More
Protein transduction from retroviral Gag precursors.
Authors:Voelkel C, Galla M, Maetzig T, Warlich E, Kuehle J, Zychlinski D, Bode J, Cantz T, Schambach A, Baum C,
Journal:Proc Natl Acad Sci U S A
PubMed ID:20385817
Retroviral particles assemble a few thousand units of the Gag polyproteins. Proteolytic cleavage mediated by the retroviral protease forms the bioactive retroviral protein subunits before cell entry. We hypothesized that this process could be exploited for targeted, transient, and dose-controlled transduction of nonretroviral proteins into cultured cells. We demonstrate that ... More
Insulin-like growth factor-1 sustains stem cell mediated renal repair.
Authors:Imberti B, Morigi M, Tomasoni S, Rota C, Corna D, Longaretti L, Rottoli D, Valsecchi F, Benigni A, Wang J, Abbate M, Zoja C, Remuzzi G,
Journal:J Am Soc Nephrol
PubMed ID:17942965
In mice with cisplatin-induced acute kidney injury, administration of bone marrow-derived mesenchymal stem cells (MSC) restores renal tubular structure and improves renal function, but the underlying mechanism is unclear. Here, we examined the process of kidney cell repair in co-culture experiments with MSC and cisplatin-injured proximal tubular epithelial cells (PTEC). ... More
Dual probe with fluorescent and magnetic properties for imaging solid tumor xenografts.
Authors:Shan L, Wang S, Sridhar R, Bhujwalla ZM, Wang PC
Journal:Mol Imaging
PubMed ID:17445503
A dual probe with fluorescent and magnetic reporter groups was constructed by linkage of the near-infrared (NIR) fluorescent transferrin conjugate (Tf(NIR)) on the surface of contrast agent-encapsulated cationic liposome (Lip-CA). This probe was used for magnetic resonance imaging (MRI) and optical imaging of MDA-MB-231-luc breast cancer cells grown as a ... More
Using total internal reflection fluorescence microscopy to visualize rhodopsin-containing cells.
Authors:
Journal:Appl Environ Microbiol
PubMed ID:25769822
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