SYBR™ Safe DNA Gel Stain in 0.5X TBE
SYBR™ Safe DNA Gel Stain in 0.5X TBE
Citas y referencias (11)
Invitrogen™

SYBR™ Safe DNA Gel Stain in 0.5X TBE

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La tinción de gel de ADN SYBR Safe se creó específicamente para reducir la mutagenicidad: ser más segura que elMás información
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Número de catálogoCantidad
S331001 l
S331014 l
Número de catálogo S33100
Precio (MXN)
-
Cantidad:
1 l
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La tinción de gel de ADN SYBR Safe se creó específicamente para reducir la mutagenicidad: ser más segura que el bromuro de etidio al teñir ADN en geles de agarosa o acrilamida. La tinción SYBR Safe no solo es menos mutagénica que el bromuro de etidio, sino que su sensibilidad de detección es también mejor. La tinción SYBR Safe se presenta como una solución premezclada que puede usarse como una solución de bromuro de etidio, ya sea en el gel durante el proceso de electroforesis o como tinción posterior al proceso. Cuando se une a los ácidos nucleicos, la tinción SYBR Safe verde fluorescente tiene una excitación de fluorescencia máxima a ∼ 280 y ∼ 502 nm, y una emisión máxima a ∼ 530 nm. La tinción de ADN SYBR Safe también está disponible en 1 l (S-33100) de solución lista para usar. El kit de inicio de tinción de gel de ADN SYBR Safe (S-33110) incluye 1 l de tinción de gel de ADN SYBR Safe y un filtro fotográfico (S-37100).

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones
Ubicación de detecciónDetección en gel
Método de detecciónFluorescencia
Características ecológicasMenos peligroso
Tipo de productoTinción para geles de ADN
Cantidad1 l
Duración de almacenamiento6 meses
Condiciones de envíoTemperatura ambiente
Molécula dianaADN
Etiqueta o tinteSYBR Safe
Unit SizeEach
Contenido y almacenamiento
• Se suministra como una solución lista para su uso en 0,5X TBE

Almacenar a temperatura ambiente en el recipiente original.

Preguntas frecuentes

Why do I sometimes see speckles in my gel when using SYBR Safe DNA Gel Stain?

Many whitening agents used in clothing, as well as some fungi and bacteria, fluoresce at the same wavelengths as SYBR Safe DNA gel stain. These contaminants within or on the surface of the gel may produce this speckling.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

What is the pH range of SYBR dyes?

The SYBR dyes are useful only over a narrow range of pH, from about 7 to 8. Outside this range, the fluorescent signal diminishes rapidly.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Which direction does the SYBR Safe dye run during electrophoresis?

Similarly to ethidium bromide, SYBR Safe DNA Gel Stain runs in the opposite direction of the migrating DNA. This has no practical effect on the use of gels cast with SYBR Safe DNA Gel Stain, as only the very bottom of the gel will have a lower concentration of stain. This effect can be partially counteracted by staining the gel with SYBR Safe DNA Gel Stain after electrophoresis. Solutions of dye should not be added to the running buffer as this can cause breakdown of the dye at the electrodes and release toxic volatile compounds into the air.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Does ethanol precipitation remove the SYBR Safe dye?

SYBR Safe DNA Gel Stain is easily removed from nucleic acids by ethanol precipitation.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Can I reuse SYBR Safe DNA Gel Stain for a second gel?

We strongly discourage the reuse of SYBR Safe DNA Gel Stain, as this practice significantly lowers sensitivity.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

View all SYBR™ Safe DNA Gel Stain in 0.5X TBE, 1 L FAQs

Citations & References (11)

Citations & References
Abstract
Features of medullary thymic epithelium implicate postnatal development in maintaining epithelial heterogeneity and tissue-restricted antigen expression.
Authors:Gillard GO, Farr AG
Journal:J Immunol
PubMed ID:16670287
'Although putative thymic epithelial progenitor cells have been identified, the developmental potential of these cells, the extent of medullary thymic epithelium (mTEC) heterogeneity, and the mechanisms that mediate the expression of a wide range of peripheral tissue-restricted Ags (TRAs) by mTECs remain poorly defined. Here we have defined several basic ... More
Biotinylated photocleavable polyethylenimine: capture and triggered release of nucleic acids from solid supports.
Authors:Handwerger RG, Diamond SL
Journal:Bioconjug Chem
PubMed ID:17432825
'A biotinylated photocleavable polyethylenimine (B-PC-PEI) was designed and synthesized for the capture and controlled release of nucleic acids from solid supports. B-PC-PEI was synthesized via a three-step reaction process and verified by 1H NMR and mass spectrometry. In aqueous solution, the o-nitrobenzyl group within B-PC-PEI was efficiently cleaved by 5 ... More
Development of a SYBR safe technique for the sensitive detection of DNA in cesium chloride density gradients for stable isotope probing assays.
Authors:Martineau C, Whyte LG, Greer CW,
Journal:J Microbiol Methods
PubMed ID:18329741
SYBR safe, a fluorescent nucleic acid stain, was evaluated as a replacement for ethidium bromide (EtBr) in cesium chloride (CsCl) density gradients for DNA stable isotope probing (DNA-SIP) assays. The separation of 12C- and 13C-labelled DNA using SYBR safe gave similar results to those obtained using EtBr with pure cultures ... More
Single step protocol to purify recombinant proteins with low endotoxin contents.
Authors:Reichelt P, Schwarz C, Donzeau M
Journal:Protein Expr Purif
PubMed ID:16290005
Endotoxin is an unwanted by product of recombinant proteins purified from Escherichia coli. The inherent toxicity of endotoxins makes their removal an important step for the proteins' application in several biological assays and for safe parenteral administration. The method described in this paper is a one-step protocol which is effective ... More
5-Methyltetrahydrofolate inhibits photosensitization reactions and strand breaks in DNA.
Authors:Offer T, Ames BN, Bailey SW, Sabens EA, Nozawa M, Ayling JE
Journal:FASEB J
PubMed ID:17341682
The known functions of folate are to support one-carbon metabolism and to serve as photoreceptors for cryptochromes and photolyases. We demonstrate that 5-methyltetrahydrofolate (5-MTHF, the predominant folate in plasma) is also a potent, near diffusion limited, scavenger of singlet oxygen and quencher of excited photosensitizers. Both pathways result in decomposition ... More
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