SYBR™ Safe DNA Gel Stain in 1X TAE
SYBR™ Safe DNA Gel Stain in 1X TAE
Citas y referencias (2)
Invitrogen™

SYBR™ Safe DNA Gel Stain in 1X TAE

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La tinción de gel de ADN SYBR Safe™ se creó específicamente para reducir la mutagenicidad: ser más segura que elMás información
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Número de catálogoCantidad
S331124 l
S331111 l
Número de catálogo S33112
Precio (MXN)
-
Cantidad:
4 l
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La tinción de gel de ADN SYBR Safe™ se creó específicamente para reducir la mutagenicidad: ser más segura que el bromuro de etidio al teñir ADN en geles de agarosa o acrilamida. La tinción SYBR Safe™ no solo es menos mutagénica que el bromuro de etidio, sino que su sensibilidad de detección también es mejor. La tinción SYBR Safe™ se presenta como una solución premezclada que puede usarse como una solución de bromuro de etidio, ya sea en el gel durante el proceso de electroforesis o como tinción posterior al proceso. Cuando se une a los ácidos nucleicos, la tinción SYBR Safe™ verde fluorescente tiene una excitación de fluorescencia máxima a ∼ 280 y ∼ 502 nm, y una emisión máxima a ∼ 530 nm.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Ubicación de detecciónDetección en gel
Método de detecciónFluorescencia
Características ecológicasMenos peligroso
Tipo de productoTinción para geles de ADN
Cantidad4 l
Duración de almacenamiento6 meses
Condiciones de envíoTemperatura ambiente
Molécula dianaADN
Etiqueta o tinteSYBR Safe
Unit SizeEach
Contenido y almacenamiento
• Se suministra como una solución lista para su uso en 1X TAE

Almacenar a temperatura ambiente en el recipiente original.

Preguntas frecuentes

Why do I sometimes see speckles in my gel when using SYBR Safe DNA Gel Stain?

Many whitening agents used in clothing, as well as some fungi and bacteria, fluoresce at the same wavelengths as SYBR Safe DNA gel stain. These contaminants within or on the surface of the gel may produce this speckling.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

What is the pH range of SYBR dyes?

The SYBR dyes are useful only over a narrow range of pH, from about 7 to 8. Outside this range, the fluorescent signal diminishes rapidly.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Which direction does the SYBR Safe dye run during electrophoresis?

Similarly to ethidium bromide, SYBR Safe DNA Gel Stain runs in the opposite direction of the migrating DNA. This has no practical effect on the use of gels cast with SYBR Safe DNA Gel Stain, as only the very bottom of the gel will have a lower concentration of stain. This effect can be partially counteracted by staining the gel with SYBR Safe DNA Gel Stain after electrophoresis. Solutions of dye should not be added to the running buffer as this can cause breakdown of the dye at the electrodes and release toxic volatile compounds into the air.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Does ethanol precipitation remove the SYBR Safe dye?

SYBR Safe DNA Gel Stain is easily removed from nucleic acids by ethanol precipitation.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Can I reuse SYBR Safe DNA Gel Stain for a second gel?

We strongly discourage the reuse of SYBR Safe DNA Gel Stain, as this practice significantly lowers sensitivity.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

View all SYBR™ Safe DNA Gel Stain in 1X TAE, 4 L FAQs

Citations & References (2)

Citations & References
Abstract
Development of a SYBR safe technique for the sensitive detection of DNA in cesium chloride density gradients for stable isotope probing assays.
Authors:Martineau C, Whyte LG, Greer CW,
Journal:J Microbiol Methods
PubMed ID:18329741
SYBR safe, a fluorescent nucleic acid stain, was evaluated as a replacement for ethidium bromide (EtBr) in cesium chloride (CsCl) density gradients for DNA stable isotope probing (DNA-SIP) assays. The separation of 12C- and 13C-labelled DNA using SYBR safe gave similar results to those obtained using EtBr with pure cultures ... More
Mechanisms of palatal epithelial seam disintegration by transforming growth factor (TGF) beta3.
Authors:Ahmed S, Liu CC, Nawshad A,
Journal:Dev Biol
PubMed ID:17698055
TGFbeta3 signaling initiates and completes sequential phases of cellular differentiation that is required for complete disintegration of the palatal medial edge seam, that progresses between 14 and 17 embryonic days in the murine system, which is necessary in establishing confluence of the palatal stroma. Understanding the cellular mechanism of palatal ... More