Medio de montaje antidecoloración SlowFade™ Gold
Medio de montaje antidecoloración SlowFade™ Gold
Citas y referencias (7)
Invitrogen™

Medio de montaje antidecoloración SlowFade™ Gold

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El medio de montaje antidecoloración SlowFade Gold es un medio de montaje líquido que se aplica directamente en muestras deMás información
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Número de catálogoCantidad
S3693610 mL
S369375 x 2 mL
S369402 mL
Número de catálogo S36936
Precio (MXN)
5,748.33
Each
Añadir al carro de la compra
Cantidad:
10 mL
Precio (MXN)
5,748.33
Each
Añadir al carro de la compra
El medio de montaje antidecoloración SlowFade Gold es un medio de montaje líquido que se aplica directamente en muestras de células o tejido etiquetadas con fluorescencia en portaobjetos de microscopio. Contiene componentes químicos diseñados para evitar que los colorantes fluorescentes se decoloren (fotoblanqueo) durante experimentos de microscopía de fluorescencia. El medio de montaje antidecoloración SlowFade Gold está hecho a base de glicerol, sin necesidad de mezcla o curado, lo que lo hace ideal para la visualización inmediata de la muestra. Listo para su uso: simplemente vierta una gota en la muestra, añada un cubreobjetos y obtenga la imagen. Está disponible con o sin tinción nuclear DAPI.

Atributos clave:
• Protege los colorantes de la decoloración durante la obtención de imágenes
• Líquido listo para usar, ideal para la visualización inmediata de la muestra
• Las muestras montadas son estables durante semanas
• Mantiene la fuerza de la señal: apenas se produce supresión
• Ideal para colorantes Alexa Fluor

Seleccione el medio de montaje antidecoloración o el reactivo clarificador óptico que se adapte a sus necesidades de adquisición de imágenes de fluorescencia ›

El medio de montaje antidecoloración SlowFade Gold no se recomienda para el montaje de muestras que contienen proteínas fluorescentes, como GFP. Para una mejor protección contra la decoloración de proteínas y colorantes fluorescentes en células cultivadas o cortes de tejido, se recomienda utilizar el medio de montaje antidecoloración SlowFade Diamond. Para obtener una alta resolución o para adquirir imágenes de tejidos más gruesos o cultivos celulares 3D con una profundidad focal de 0–500 µm, pruebe el medio de montaje SlowFade Glass.

Seleccione el mejor reactivo antidecoloración SlowFade para las necesidades de sus experimentos ›
For Research Use Only. Not for use in diagnostic procedures.
Especificaciones
Características ecológicasEmbalaje sostenible menos peligroso
Línea de productosSlowFade
Cantidad10 mL
Condiciones de envíoTemperatura ambiente
Para utilizar con (equipo)Microscopio
FormularioLíquido
Tipo de productoMontaje antidecoloración
Tipo de soluciónSolución antidecoloración
Unit SizeEach
Contenido y almacenamiento
Se recomienda el almacenamiento a temperatura ambiente, pero también se puede almacenar congelado (entre -5 y -30 °C). Proteja el producto de la luz.

Preguntas frecuentes

What is the difference between ProLong and SlowFade antifade reagents?

Our ProLong antifade reagents dispense as a liquid that will solidify upon the evaporation of water. SlowFade antifade reagents remain liquid. If you are going to image right away and then dispose of your sample, you do not need a mountant that hardens, such as the SlowFade reagents. If you wish to archive your slide for more than a day, you will want a mounting medium that hardens (or “cures”). This hardening will limit the off-rates of various dye-conjugated antibodies and provides a better refractive index. Also, there will be a lower diffusion rate of free radicals, limiting photobleaching.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Some antifade mounting media stay as liquid whereas others harden. What is the benefit of having one that hardens?

If you are going to image right away and then dispose of your sample, you probably want a mountant that does not harden. If you wish to archive your slide for more than a day, you want a mountant that hardens (or "cures"). This hardening will slow or prevent off-rate of your dye or conjugate and often produces a better refractive index. Secondary sealing is usually not necessary. Also there will be lower diffusion of free radicals, thus limiting photobleaching.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I do not have epoxy or VALAP to seal the coverslip. Do you offer an alternative coverslip sealant?

We offer ProLong Coverslip Sealant (Cat. No. P56128) that can be used to seal the edges of the coverslip and is compatible with both curing and non-curing mountant. The sealant is easy to apply and is brushed on after the mountant has cured, for long preservation of slides. The product page can be found here.

When using a hard-curing mountant, such as ProLong Antifade Mountant, make sure the mountant is fully cured before applying ProLong Coverslip Sealant. Since this sealant can seal moisture under the coverslip, it can interfere with the curing process.

When using a non-curing mountant, such as SlowFade Antifade Mountants, sealing can take place immediately after mounting.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Does Slowfade Antifade Mountant require using a nail polish for sealing?

No. Samples mounted with SlowFade mountants need not be sealed; they are intended for immediate viewing. The coverslip may be anchored (to prevent movement while viewing) by applying molten paraffin to three or four spots around the edge of the coverslip.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (7)

Citations & References
Abstract
Reactive oxygen species production via NADPH oxidase mediates TGF-beta-induced cytoskeletal alterations in endothelial cells.
Authors:Hu T, Ramachandrarao SP, Siva S, Valancius C, Zhu Y, Mahadev K, Toh I, Goldstein BJ, Woolkalis M, Sharma K
Journal:Am J Physiol Renal Physiol
PubMed ID:16159901
'Cytoskeletal alterations in endothelial cells have been linked to nitric oxide generation and cell-cell interactions. Transforming growth factor (TGF)-beta has been described to affect cytoskeletal rearrangement in numerous cell types; however, the underlying pathway is unclear. In the present study, we found that human umbilical vein endothelial cells (HUVEC) have ... More
Identification and characterization of small molecules that inhibit intracellular toxin transport.
Authors:Saenz JB, Doggett TA, Haslam DB
Journal:Infect Immun
PubMed ID:17576758
'Shiga toxin (Stx), cholera toxin (Ctx), and the plant toxin ricin are among several toxins that reach their intracellular destinations via a complex route. Following endocytosis, these toxins travel in a retrograde direction through the endosomal system to the trans-Golgi network, Golgi apparatus, and endoplasmic reticulum (ER). There the toxins ... More
Rac-mediated macropinocytosis is a critical route for naked plasmid DNA transfer in mice.
Authors:Fumoto S, Nishi J, Ishii H, Wang X, Miyamoto H, Yoshikawa N, Nakashima M, Nakamura J, Nishida K,
Journal:Mol Pharm
PubMed ID:19492848
We have recently discovered the potential for in vivo naked plasmid DNA (pDNA) transfer into gastric serosal surface cells in mice. As pDNA are huge molecules, the mechanism of gene transfer without carriers and physical forces is of great biological interest. The endocytic route for naked pDNA transfer into gastric ... More
Programmable in situ amplification for multiplexed imaging of mRNA expression.
Authors:Choi HM, Chang JY, Trinh le A, Padilla JE, Fraser SE, Pierce NA,
Journal:Nat Biotechnol
PubMed ID:21037591
In situ hybridization methods enable the mapping of mRNA expression within intact biological samples. With current approaches, it is challenging to simultaneously map multiple target mRNAs within whole-mount vertebrate embryos, representing a significant limitation in attempting to study interacting regulatory elements in systems most relevant to human development and disease. ... More
A protocol for dissecting Drosophila melanogaster brains for live imaging or immunostaining.
Authors:Wu JS, Luo L
Journal:Nat Protoc
PubMed ID:17487202
This protocol describes a basic method for dissection and immunofluorescence staining of the Drosophila brain at various developmental stages. The Drosophila brain has become increasingly useful for studies of neuronal wiring and morphogenesis in combination with techniques such as the 'mosaic analysis with a repressible cell marker' (MARCM) system, where ... More
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