El conjugado de estreptavidina Texas Red™ tiene una excitación y emisión máxima de ∼ 596/612 nm y se utiliza ampliamenteMás información
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Número de catálogo
Cantidad
S872
1 mg
Número de catálogo S872
Precio (MXN)
-
Cantidad:
1 mg
El conjugado de estreptavidina Texas Red™ tiene una excitación y emisión máxima de ∼ 596/612 nm y se utiliza ampliamente para detectar sondas biotiniladas por microscopía de fluorescencia.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Tipo de productoConjugado de estreptavidina (fluorescente)
Cantidad1 mg
Condiciones de envíoTemperatura ambiente
ConjugadoTexas Red
FormularioSólido
Línea de productosTexas Red
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador (de -5 a -30 °C) y proteger de la luz.
Preguntas frecuentes
I am planning to use a fluorescent streptavidin labeled conjugate. What are the storage conditions and shelf life for the lyophilized powder and reconstituted solution?
In the lyophilized powder form, the fluorescent streptavidin labeled conjugate is stable for six months when stored at -20 degrees C, desiccated, and protected from light. The reconstituted solution is stable for approximately six months when stored at 4 degrees C, protected from light, with the addition of sodium azide to a final concentration of 5 mM or thimerosal to 0.2 mM. For longer storage, we recommend dividing the solution into aliquots and freezing at -20 degrees C, protected from light. Avoid repeated freezing and thawing of the solution.
I am planning to use a fluorescent streptavidin labeled conjugate. How should I prepare the working solution of the conjugate?
The fluorescent streptavidin labeled conjugate solution can be made by dissolving the powder in 0.5-1.0 mL of PBS or other suitable buffer. For details, please refer to page 4 of the "Streptavidin and Fluorescent Conjugates of Streptavidin" manual (https://assets.fishersci.com/TFS-Assets/LSG/manuals/mp00888.pdf).
Citations & References (100)
Citations & References
Abstract
The zinc finger protein A20 inhibits TNF-induced NF-kappaB-dependent gene expression by interfering with an RIP- or TRAF2-mediated transactivation signal and directly binds to a novel NF-kappaB-inhibiting protein ABIN.
The zinc finger protein A20 is a tumor necrosis factor (TNF)â and interleukin 1 (IL-1)-inducible protein that negatively regulates nuclear factor-kappa B (NF-κB)âdependent gene expression. However, the molecular mechanism by which A20 exerts this effect is still unclear. We show that A20 does not inhibit TNF- induced nuclear translocation and ... More
In situ polymerase chain reaction amplification of HIV-1 DNA in brain tissue.
Authors:Strappe PM, Wang TH, McKenzie CA, Lowrie S, Simmonds P, Bell JE
Journal:J Virol Methods
PubMed ID:9562406
A direct in situ polymerase chain reaction (IS-PCR) assay is described for the detection of HIV-1 proviral DNA in formalin fixed paraffin embedded brain tissue. Biotin-16-dUTP is incorporated during the PCR process and microwave pretreatment of tissue sections ensures that no non-specific incorporation into damaged or nicked genomic DNA occurs. ... More
Organization of long-range inhibitory connections with rat visual cortex.
Authors:McDonald CT, Burkhalter A
Journal:J Neurosci
PubMed ID:7678860
We have studied the laminar organization of local long-range inhibitory connections within rat primary visual cortex (area 17) by combining retrograde tracing of nerve cell bodies with glutamic acid decarboxylase immunocytochemistry. While most inhibitory connections are confined to within 0.4 mm of the injection site, a subset of neurons at ... More
Fibronectin is a component of the sodium dodecyl sulfate-insoluble transglutaminase substrate.
Authors:Tyrrell DJ, Sale WS, Slife CW
Journal:J Biol Chem
PubMed ID:2897368
Liver plasma membranes contain a morphologically distinct protein complex which serves as a substrate for the plasma membrane-associated transglutaminase. The complex, which appears as a two-dimensional sheet, is insoluble in sodium dodecyl sulfate and reducing agents and has been named SITS for sodium dodecyl sulfate-insoluble transglutaminase substrate (Tyrrell, D. J., ... More
Autoantigens targeted in systemic lupus erythematosus are clustered in two populations of surface structures on apoptotic keratinocytes.
Authors:Casciola-Rosen LA, Anhalt G, Rosen A
Journal:J Exp Med
PubMed ID:7511686
'Systemic lupus erythematosus is a multisystem autoimmune disease in which the autoantibody response targets a variety of autoantigens of diverse subcellular location. We show here that these autoantigens are clustered in two distinct populations of blebs at the surface of apoptotic cells. The population of smaller blebs contains fragmented endoplasmic ... More