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Citas y referencias (28)
Invitrogen™
Streptavidin, Alkaline Phosphatase Conjugate
La estreptavidina Thermo Scientific, conjugado de fosfatasa alcalina, incluye estreptavidina en forma purificada, conjugada con fosfatasa alcalina para la detecciónMás información
| Número de catálogo | Cantidad |
|---|---|
| S921 | 0,5 mL |
Número de catálogo S921
Precio (MXN)
-
Cantidad:
0,5 mL
La estreptavidina Thermo Scientific, conjugado de fosfatasa alcalina, incluye estreptavidina en forma purificada, conjugada con fosfatasa alcalina para la detección basada en sustrato.
La estreptavidina es una proteína de unión a biotina aislada originalmente de Streptomyces avidinii. A diferencia de la avidina, la estreptavidina no tiene carbohidratos y tiene un pl ligeramente ácido de 5. Los productos de estreptavidina de Thermo Scientific utilizan un formato de estreptavidina recombinante con una masa de 53 000 dalton y un pl casi neutro. La estreptavidina es una proteína tetramérica, con cada subunidad que une una molécula de biotina con afinidad similar a la de la avidina. El cloruro de guanidinio disociará la avidina y la estreptavidina en subunidades, pero la estreptavidina es más resistente a la disociación.
La estreptavidina, conjugado de fosfatasa alcalina, permite la detección de anticuerpos biotinilados y otras sondas en una variedad de métodos de ensayo estándar, incluidos Western blot, ELISA, inmunohistoquímica y adquisición de imágenes de fluorescencia.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Concentración2 mg/mL
Etiqueta o tinteAP (fosfatasa alcalina)
Tipo de productoConjugado de estreptavidina (fluorescente)
Cantidad0,5 mL
Condiciones de envíoHielo húmedo
ConjugadoAP
FormularioLíquido
Unit SizeEach
Contenido y almacenamiento
Almacenar en el refrigerador (2–8° C).
Citations & References (28)
Citations & References
Abstract
Enumeration of antigen-specific IgE responses at the single-cell level by an ELISA plaque assay.
Journal:J Immunol Methods
PubMed ID:1703186
In vitro elicitation and enumeration of antigen-specific IgE-secreting cells in antigen-primed murine lymphocyte cultures can be reproducibly and accurately quantitated. Rat anti-mouse IgE monoclonal antibodies (Mab anti-epsilon) were developed. Antigen-specific IgE-secreting cells can be detected on antigen or MAb anti-epsilon coated nitrocellulose discs pasted to 24-well culture plates. This IgE
Detection of low copy numbers of HPV DNA by fluorescent in situ hybridization combined with confocal microscopy as an alternative to in situ polymerase chain reaction.
Journal:J Virol Methods
PubMed ID:9672129
'In genital lesions infected by human papillomavirus (HPV), histological criteria and HPV DNA typing are of prognostic value. Therefore, non-radioactive methods such as in situ hybridization are used extensively since they preserve the histological organization of the tissue, and allow the detection and characterization of HPV DNA. However, the sensitivity
Easily reversible desthiobiotin binding to streptavidin, avidin, and other biotin-binding proteins: uses for protein labeling, detection, and isolation.
Journal:Anal Biochem
PubMed ID:12419349
'The high-affinity binding of biotin to avidin, streptavidin, and related proteins has been exploited for decades. However, a disadvantage of the biotin/biotin-binding protein interaction is that it is essentially irreversible under physiological conditions. Desthiobiotin is a biotin analogue that binds less tightly to biotin-binding proteins and is easily displaced by
Binding of monoclonal antibody AA4 to gangliosides on rat basophilic leukemia cells produces changes similar to those seen with Fc epsilon receptor activation.
Journal:J Cell Biol
PubMed ID:1370498
'The mAb AA4 binds to novel derivatives of the ganglioside Gd1b on rat basophilic leukemia (RBL-2H3) cells. Some of the gangliosides are located close to the high affinity IgE receptor (Fc epsilon RI), and binding of mAb AA4 inhibits Fc epsilon RI-mediated histamine release. In the present study, mAb AA4
Ubiquitination and proteasomal activity is required for transport of the EGF receptor to inner membranes of multivesicular bodies.
Journal:J Cell Biol
PubMed ID:11864992
'EGF, but not TGF alpha, efficiently induces degradation of the EGF receptor (EGFR). We show that EGFR was initially polyubiquitinated to the same extent upon incubation with EGF and TGF alpha, whereas the ubiquitination was more sustained by incubation with EGF than with TGF alpha. Consistently, the ubiquitin ligase c-Cbl