Tubulin Tracker™ Green (Oregon Green™ 488 Taxol, bis-acetato)

Citas y referencias (9)

Invitrogen™

Tubulin Tracker™ Green (Oregon Green™ 488 Taxol, bis-acetato)

Tubulin Tracker Green (Oregon Green 488 Taxol, bis-acetato) proporciona un color verde-fluorescente de tubulina polimerizada en células vivas. Tubulin TrackerMás información

Número de catálogo	Cantidad
T34078	60 portaobjetos

Número de catálogo T34078

Precio (MXN)

Cantidad:

60 portaobjetos

Tubulin Tracker Green (Oregon Green 488 Taxol, bis-acetato) proporciona un color verde-fluorescente de tubulina polimerizada en células vivas. Tubulin Tracker Green es un compuesto no cargado, no fluorescente que pasa fácilmente a través de la membrana plasmática de células vivas. Una vez dentro de la célula, el grupo de bloqueo lipofílico se escinde con esterasas no específicas, lo que resulta en una forma Cardada verde-fluorescente, que impide que escape de las células vivas. El reactivo TubulinTracker Green utiliza el tinte Oregon Green 488, que absorbe y emite de forma óptima a 494 nm y 522 nm, respectivamente, y la fluorescencia se puede visualizar con ajustes de filtro estándar GFP/FITC utilizando prácticamente cualquier instrumento de adquisición de imágenes fluorescentes. Se puede multiplexar con fluoróforos azules, naranjas, rojos, rojos intensos y de infrarrojo cercano.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Tipo de coloranteOregon Green™ 488

Intervalo de longitud de onda de excitación494/522

Línea de productosTubulinTracker

Cantidad60 portaobjetos

Condiciones de envíoAprobado para su envío en hielo húmedo o seco

Tipo de etiquetaEtiquetas UV-Vis

Tipo de productoTubulin Tracker

SubCellular LocalizationTubulina

Unit Size60 slides

Contenido y almacenamiento

Contiene material suficiente para al menos 60 portaobjetos a 1 ml/portaobjetos y 1 x de concentración de trabajo.

1 x 20 μg de Tubulin Tracker Green (taxol Oregon Green 488, bis-acetato)

1 x 1 ml de solución plurónica F-127, 20 % en DMSO

Preguntas frecuentes

What is the difference between Tubulin Tracker Green (Cat No. T34078) and Tubulin Tracker Green, for live cell imaging (Cat. No. T34075)?

Both products include the same Tubulin Tracker Green reagent. The difference is in the packaging and amount of Tubulin Tracker Green reagent contained. Tubulin Tracker Green, for live cell imaging (Cat. No. T34075) includes enough Tubulin Tracker Green reagent for 300 slides whereas Tubulin Tracker Green (Cat No. T34078) contains enough Tubulin Tracker Green reagent for 60 slides. Also, Tubulin Tracker Green, for live cell imaging (Cat. No. T34075) does not include Probenecid.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

With Tubulin Tracker Deep Red and Tubulin Tracker Green, can I fix samples after labeling?

No. The labeling is not retained upon fixation. Imaging of the samples should be done only on live cells. To limit photobleaching while viewing live cells, use ProLong Live Antifade Reagent, for live cell imaging (Cat No. P36974 and P36975).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How is Docetaxel in Tubulin Tracker Deep Red different from Taxol in Tubulin Tracker Green? Are there any differences in the binding or cytotoxicity between the two reagents?

Docetaxel has a slightly higher binding affinity than Taxol and exhibits longer retention time due to better uptake and slower efflux, but for use in labeling cultured cells, these differences should be minimal. More information comparing the properties of these two reagents is provided in this publication: https://www.ncbi.nlm.nih.gov/pubmed/1671606 https://www.cell.com/fulltext/S1074-5521(05)00301-7

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (9)

Citations & References

Abstract

Involvement of 14-3-3 in tubulin instability and impaired axon development is mediated by Tau.

Authors:Joo Y, Schumacher B, Landrieu I, Bartel M, Smet-Nocca C, Jang A, Choi HS, Jeon NL, Chang KA, Kim HS, Ottmann C, Suh YH,

Journal:

PubMed ID:26103986

'14-3-3 proteins act as adapters that exert their function by interacting with their various protein partners. 14-3-3 proteins have been implicated in a variety of human diseases including neurodegenerative diseases. 14-3-3 proteins have recently been reported to be abundant in the neurofibrillary tangles (NFTs) observed inside the neurons of brains ... More

Impact of C24:0 on actin-microtubule interaction in human neuronal SK-N-BE cells: evaluation by FRET confocal spectral imaging microscopy after dual staining with rhodamine-phalloidin and tubulin tracker green.

Authors:Zarrouk A, Nury T, Dauphin A, Frère P, Riedinger JM, Bachelet CM, Frouin F, Moreau T, Hammami M, Kahn E, Lizard G,

Journal:

PubMed ID:26214025

'Disorganization of the cytoskeleton of neurons has major consequences on the transport of neurotransmitters via the microtubule network. The interaction of cytoskeleton proteins (actin and tubulin) was studied in neuronal SK-N-BE cells treated with tetracosanoic acid (C24:0), which is cytotoxic and increased in Alzheimer''s disease patients. When SK-N-BE cells were ... More

The Ovary of Tubifex tubifex (Clitellata, Naididae, Tubificinae) Is Composed of One, Huge Germ-Line Cyst that Is Enriched with Cytoskeletal Components.

Authors:Urbisz AZ, Chajec L, Swiatek P,

Journal:

PubMed ID:26001069

Recent studies on the ovary organization and oogenesis in Tubificinae have revealed that their ovaries are small polarized structures that are composed of germ cells in subsequent stages of oogenesis that are associated with somatic cells. In syncytial cysts, as a rule, each germ cell is connected to the central ... More

Multimodal two-photon imaging using a second harmonic generation-specific dye.

Authors:Nuriya M, Fukushima S, Momotake A, Shinotsuka T, Yasui M, Arai T,

Journal:Nat Commun

PubMed ID:27156702

'Second harmonic generation (SHG) imaging can be used to visualize unique biological phenomena, but currently available dyes limit its application owing to the strong fluorescent signals that they generate together with SHG. Here we report the first non-fluorescent and membrane potential-sensitive SHG-active organic dye Ap3. Ap3 is photostable and generates ... More

Tubulin is a molecular target of the Wnt-activating chemical probe.

Authors:Fukuda Y, Sano O, Kazetani K, Yamamoto K, Iwata H, Matsui J,

Journal:BMC Biochem

PubMed ID:27207629

In drug discovery research, cell-based phenotypic screening is an essential method for obtaining potential drug candidates. Revealing the mechanism of action is a key step on the path to drug discovery. However, elucidating the target molecules of hit compounds from phenotypic screening campaigns remains a difficult and troublesome process. Simple ... More

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