Paquete de variedades Tubulin Tracker™

El paquete variado de Tubulin Tracker es un kit que contiene Tubulin Tracker Green y Tubulin Tracker Deep Red, queMás información

Número de catálogo	Cantidad
T34079	2 x 60 portaobjetos

Número de catálogo T34079

Precio (MXN)

Cantidad:

2 x 60 portaobjetos

El paquete variado de Tubulin Tracker es un kit que contiene Tubulin Tracker Green y Tubulin Tracker Deep Red, que proporcionan fluorescencia verde y roja respectivamente, cuando se unen a la tubulina polimerizada en células vivas. Tubulin Tracker Green proporciona fluorescencia con un máximo de excitación de 494 nm y un máximo de emisión de 522 nm y puede detectarse con ajustes de filtro FITC/GFP estándar utilizando un microscopio de fluorescencia. Tubulin Tracker Deep Red proporciona fluorescencia con un máximo de excitación de 652 nm y un máximo de emisión de 669 nm, y puede detectarse utilizando ajustes de filtro Cy5 estándar.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Intervalo de longitud de onda de excitaciónTubulin Tracker Deep Red: 652/669, Tubulin Tracker Green: 494/522

Línea de productosTubulinTracker

Cantidad2 x 60 portaobjetos

Condiciones de envíoAprobado para su envío en hielo húmedo o seco

Tipo de etiquetaEtiquetas UV-Vis

Tipo de productoTubulin Tracker

SubCellular LocalizationTubulina

Unit Size2 x 60 slides

Contenido y almacenamiento

Contiene material suficiente para al menos 60 portaobjetos para Tubulin Tracker Green y Tubulin Tracker Deep Red a 1 ml/portaobjetos y 1 x de concentración de trabajo.Este kit contiene un kit de Tubulin Tracker Green (n.º de cat. T34078) y un

Preguntas frecuentes

What is the difference between Tubulin Tracker Green (Cat No. T34078) and Tubulin Tracker Green, for live cell imaging (Cat. No. T34075)?

Both products include the same Tubulin Tracker Green reagent. The difference is in the packaging and amount of Tubulin Tracker Green reagent contained. Tubulin Tracker Green, for live cell imaging (Cat. No. T34075) includes enough Tubulin Tracker Green reagent for 300 slides whereas Tubulin Tracker Green (Cat No. T34078) contains enough Tubulin Tracker Green reagent for 60 slides. Also, Tubulin Tracker Green, for live cell imaging (Cat. No. T34075) does not include Probenecid.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

With Tubulin Tracker Deep Red and Tubulin Tracker Green, can I fix samples after labeling?

No. The labeling is not retained upon fixation. Imaging of the samples should be done only on live cells. To limit photobleaching while viewing live cells, use ProLong Live Antifade Reagent, for live cell imaging (Cat No. P36974 and P36975).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How is Docetaxel in Tubulin Tracker Deep Red different from Taxol in Tubulin Tracker Green? Are there any differences in the binding or cytotoxicity between the two reagents?

Docetaxel has a slightly higher binding affinity than Taxol and exhibits longer retention time due to better uptake and slower efflux, but for use in labeling cultured cells, these differences should be minimal. More information comparing the properties of these two reagents is provided in this publication: https://www.ncbi.nlm.nih.gov/pubmed/1671606 https://www.cell.com/fulltext/S1074-5521(05)00301-7

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Is Tubulin Tracker Deep Red acetylated? Does it require intracellular esterase activity to make the reagent active?

Tubulin Tracker Deep Red is not acetylated. It is inherently cell permeable and is not modified further by any cellular enzymatic activity to fluoresce or bind to actin.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Do I need to use Probenecid when using Tubulin Tracker Deep Red?

The use of Probenecid is optional. Its requirement is dependent upon the cell type. Some cell types actively efflux the reagent out of the cells within a short time, limiting detection, other cell types do not exhibit this activity or exhibit very limited activity.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.