Vybrant™ Cell Metabolic Assay Kit, with C12-resazurin
Vybrant&trade; Cell Metabolic Assay Kit, with C<sub>12</sub>-resazurin
Citas y referencias (7)
Invitrogen™

Vybrant™ Cell Metabolic Assay Kit, with C12-resazurin

En el kit de ensayo de células metabólicas Vybrant™, las células viables reducen la C12-resazurina no fluorescente a C12-resorufina; laMás información
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Número de catálogoCantidad
V231101 kit
Número de catálogo V23110
Precio (MXN)
-
Cantidad:
1 kit
En el kit de ensayo de células metabólicas Vybrant™, las células viables reducen la C12-resazurina no fluorescente a C12-resorufina; la señal resultante es proporcional al número de células presentes. La C12-resazurina tiene mejor retención celular que el colorante de color azul de alamarBlue™ y se puede multiplexar con indicadores de viabilidad y otros biomarcadores
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Método de detecciónFluorescente
Tipo de coloranteResorufina
FormatoTubos, placa de 96 pocillos
Cantidad1 kit
Condiciones de envíoTemperatura ambiente
Para utilizar con (aplicación)Ensayo metabólico celular
Para utilizar con (equipo)Citómetro de flujo, Lector de microplacas
Línea de productosVybrant
Tipo de productoTinte
Unit Size1 kit
Contenido y almacenamiento
Almacenar en el congelador de -5 °C a -30 °C y proteger de la luz.

Preguntas frecuentes

How do alamarBlue reagent and PrestoBlue reagent differ from resazurin and C12-resazurin?

alamarBlue reagent and PrestoBlue reagent contain resazurin in a proprietary stabilizing formulation that allows for a convenient “mix, incubate, and read” protocol. PrestoBlue reagent is an improvement in the formulation of alamarBlue reagent that allows for much faster staining (typically 10 minutes vs. 1-4 hours to obtain a similar signal and sensitivity). C12-resazurin is a derivative of resazurin that has better cellular retention and thus allows for analysis on a flow cytometer and multiplexing with viability indicators and other biomarkers.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the absorbance/emission of the reaction product from the Vybrant Cell Metabolic Assay Kit?

The absorbance/emission is 563/587 nm.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (7)

Citations & References
Abstract
Membrane disruption by antimicrobial fatty acids releases low-molecular-weight proteins from Staphylococcus aureus.
Authors:Parsons JB, Yao J, Frank MW, Jackson P, Rock CO,
Journal:J Bacteriol
PubMed ID:22843840
The skin represents an important barrier for pathogens and is known to produce fatty acids that are toxic toward gram-positive bacteria. A screen of fatty acids as growth inhibitors of Staphylococcus aureus revealed structure-specific antibacterial activity. Fatty acids like oleate (18:1?9) were nontoxic, whereas palmitoleate (16:1?9) was a potent growth ... More
Perturbational profiling of nanomaterial biologic activity.
Authors:Shaw SY, Westly EC, Pittet MJ, Subramanian A, Schreiber SL, Weissleder R,
Journal:Proc Natl Acad Sci U S A
PubMed ID:18492802
'Our understanding of the biologic effects (including toxicity) of nanomaterials is incomplete. In vivo animal studies remain the gold standard; however, widespread testing remains impractical, and the development of in vitro assays that correlate with in vivo activity has proven challenging. Here, we demonstrate the feasibility of analyzing in vitro ... More
Novel designs of multivalent anti-CD20 humanized antibodies as improved lymphoma therapeutics.
Authors:Rossi EA, Goldenberg DM, Cardillo TM, Stein R, Wang Y, Chang CH,
Journal:Cancer Res
PubMed ID:18922911
'Multivalent antibodies, either monospecific or bispecific, may improve the efficacy of current therapeutic interventions involving a single monoclonal antibody (mAb). We have applied the Dock-and-Lock (DNL) method, a new platform technology for the site-specific and covalent assembly of modular components into stably tethered complexes of defined composition, to prepare a ... More
Preclinical studies on targeted delivery of multiple IFNa2b to HLA-DR in diverse hematologic cancers.
Authors:Rossi EA, Rossi DL, Cardillo TM, Stein R, Goldenberg DM, Chang CH,
Journal:Blood
PubMed ID:21680794
The short circulating half-life and side effects of IFNa affect its dosing schedule and efficacy. Fusion of IFNa to a tumor-targeting mAb (mAb-IFNa) can enhance potency because of increased tumor localization and improved pharmacokinetics. We used the Dock-and-Lock method to generate C2-2b-2b, a mAb-IFNa comprising tetrameric IFNa2b site-specifically linked to ... More
Hypothermia reduces secretion of vascular endothelial growth factor by cultured retinal pigment epithelial cells.
Authors:Coassin M, Duncan KG, Bailey KR, Singh A, Schwartz DM,
Journal:Br J Ophthalmol
PubMed ID:20805126
Visual loss in age-related macular degeneration usually develops secondary to choroidal neovascularisation. Vascular endothelial growth factor (VEGF) is a critical regulator of retinal angiogenesis and vascular permeability, especially in hypoxic conditions. We hypothesise that hypothermia may reduce the retinal pigment epithelium (RPE) metabolism and, consequently, the levels of VEGF secretion ... More
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