Informational:The SiteClick™ sDIBO Alkyne Kits are optimized to be used in conjunction with our SiteClick™ Antibody Azido Modification Kits
Invitrogen™

SiteClick™ sDIBO Alkyne Kits for Antibody Labeling

Catalog number: S10914
Invitrogen™

SiteClick™ sDIBO Alkyne Kits for Antibody Labeling

Catalog number: S10914
Generate site-specific labeled antibody conjugates with SiteClick sDIBO Alkynes for Antibody Labeling. Our SiteClick sDIBO Antibody Labeling kits are optimized to be used in conjunction with the SiteClick Azido Modification kits (S20026, S10900, or S10901 sold separately) and contain sufficient reagents to perform a conjugation reaction of a SiteClick sDIBO Alkyne label using 100 µg, 1 mg, or 5 mg of azido-modified IgG antibody. The SiteClick sDIBO Alkynes for Antibody Labeling employs copper-free click chemistry to covalently link a dibenzocyclooctyne (sDIBO) labeled Alexa Fluor dye, pHrodo dye, or biotin to an azido modified antibody. The label, when attached to the antibody, is located far away from its antigen binding domain.
 
Catalog Number
S10914
Unit Size
For up to 250 µg of Ab
Label or Dye
pHrodo™ Deep Red
Labeling Scale
100-250 µg
Price (USD)
Price: 347.00
Your Price:
Availability
-
Full specifications
Quantity
Kit ContentsContains 1 vial of SiteClick pHrodo Deep Red sDIBO Alkyne for Antibody Labeling in 25 µL DMSO, 1 dye removal column, 1 wash vial, 1 collection vial, and PBS exchange buffer
Filter TypeCy5
Chemical ReactivityAzide
Flow Cytometer Laser LinesRed
Labeling MethodClick Chemistry
Labeling Scale100-250 µg
Shipping ConditionWet Ice
Reactive MoietysDIBO Alkyne
Label or DyepHrodo™ Deep Red
ColorDeep Red
Product LinepHrodo™, SiteClick
Labeling TargetAzido modified antibodies
Excitation/Emission640/655
Unit SizeFor up to 250 µg of Ab
Catalog Number
Unit Size
1 each
Label or Dye
Alexa Fluor™ 488
Labeling Scale
100-250 µg
Price (USD)
Price: 274.00
Your Price:
Availability
-
Full specifications
Quantity
Kit ContentsContains 1 vial of SiteClick Alexa Fluor 488 sDIBO Alkyne for Antibody Labeling in 25 µL DMSO and 1 antibody concentrator
Filter TypeGFP
Chemical ReactivityAzide
Flow Cytometer Laser LinesBlue
Labeling MethodClick Chemistry
Labeling Scale100-250 µg
Shipping ConditionWet Ice
Reactive MoietysDIBO Alkyne
Label or DyeAlexa Fluor™ 488
ColorGreen
Product LineAlexa Fluor™, SiteClick
Labeling TargetAzido modified antibodies
Excitation/Emission490/525
Unit Size1 each
Catalog Number
Unit Size
1 each
Label or Dye
Alexa Fluor™ 647
Labeling Scale
100-250 µg
Price (USD)
Price: 274.00
Your Price:
Availability
-
Full specifications
Quantity
Kit ContentsContains 1 vial of SiteClick Alexa Fluor 647 sDIBO Alkyne for Antibody Labeling in 25 µL DMSO and 1 antibody concentrator
Filter TypeCy5
Chemical ReactivityAzide
Flow Cytometer Laser LinesRed
Labeling MethodClick Chemistry
Labeling Scale100-250 µg
Shipping ConditionWet Ice
Reactive MoietysDIBO Alkyne
Label or DyeAlexa Fluor™ 647
ColorFar-Red
Product LineAlexa Fluor™, SiteClick
Labeling TargetAzido modified antibodies
Excitation/Emission650/665
Unit Size1 each
Catalog Number
Unit Size
1 each
Label or Dye
Primary Amine
Labeling Scale
100-250 µg
Price (USD)
Price: 173.00
Your Price:
Availability
-
Full specifications
Quantity
Kit ContentsContains 1 vial of SiteClick Amine sDIBO Alkyne for Antibody Labeling in 25 µL DMSO and 1 antibody concentrator
Chemical ReactivityAzide
Labeling MethodClick Chemistry
Labeling Scale100-250 µg
Shipping ConditionWet Ice
Reactive MoietysDIBO Alkyne
Label or DyePrimary Amine
Product LineSiteClick
Labeling TargetAzido modified antibodies
Unit Size1 each
Catalog Number
Unit Size
1 kit
Label or Dye
pHrodo™ iFL Red
Labeling Scale
100-250 µg
Price (USD)
Price: 308.00
Your Price:
Availability
-
Full specifications
Quantity
Kit ContentsContains 1 vial of SiteClick pHrodo iFL Red sDIBO Alkyne for Antibody Labeling, 1 vial of DMSO, and 1 antibody concentrator
Filter TypeRFP, Texas Red
Chemical ReactivityAzide
Flow Cytometer Laser LinesBlue, Yellow
Labeling MethodClick Chemistry
Labeling Scale100-250 µg
Shipping ConditionWet Ice
Reactive MoietysDIBO Alkyne
Label or DyepHrodo™ iFL Red
ColorRed
Product LinepHrodo™, SiteClick
Labeling TargetAzido modified antibodies
Excitation/Emission560/585
Unit Size1 kit
Catalog Number
Unit Size
for 1 mg of Ab
Label or Dye
Biotin
Labeling Scale
1 mg
Price (USD)
Price: 309.00
Your Price:
Availability
-
Full specifications
Quantity
Kit ContentsContains 1 vial of SiteClick Biotin sDIBO Alkyne for Antibody Labeling in 50 µL DMSO and 1 antibody concentrator
Chemical ReactivityAzide
Labeling MethodClick Chemistry
Labeling Scale1 mg
Shipping ConditionWet Ice
Reactive MoietysDIBO Alkyne
Label or DyeBiotin
Product LineAlexa Fluor™, SiteClick
Labeling TargetAzido modified antibodies
Unit Sizefor 1 mg of Ab
Catalog Number
Unit Size
for 1 mg of Ab
Label or Dye
pHrodo™ iFL Red
Labeling Scale
1 mg
Price (USD)
Price: 524.00
Your Price:
Availability
-
Full specifications
Quantity
Kit ContentsContains 1 vial of SiteClick pHrodo iFL Red sDIBO Alkyne for Antibody Labeling, 1 vial of DMSO, and 1 antibody concentrator
Filter TypeRFP, Texas Red
Chemical ReactivityAzide
Flow Cytometer Laser LinesBlue, Yellow
Labeling MethodClick Chemistry
Labeling Scale1 mg
Shipping ConditionWet Ice
Reactive MoietysDIBO Alkyne
Label or DyepHrodo™ iFL Red
ColorRed
Product LinepHrodo™, SiteClick
Labeling TargetAzido modified antibodies
Excitation/Emission560/585
Unit Sizefor 1 mg of Ab
Catalog Number
Unit Size
for 1 mg of Ab
Label or Dye
Alexa Fluor™ 488
Labeling Scale
1 mg
Price (USD)
Price: 428.00
Your Price:
Availability
-
Full specifications
Quantity
Kit ContentsContains 1 vial of SiteClick Alexa Fluor 488 sDIBO Alkyne for Antibody Labeling in 50 µL DMSO and 1 antibody concentrator
Filter TypeGFP
Chemical ReactivityAzide
Flow Cytometer Laser LinesBlue
Labeling MethodClick Chemistry
Labeling Scale1 mg
Shipping ConditionWet Ice
Reactive MoietysDIBO Alkyne
Label or DyeAlexa Fluor™ 488
ColorGreen
Product LineAlexa Fluor™, SiteClick
Labeling TargetAzido modified antibodies
Excitation/Emission490/525
Unit Sizefor 1 mg of Ab
Catalog Number
Unit Size
for 1 mg of Ab
Label or Dye
Alexa Fluor™ 647
Labeling Scale
1 mg
Price (USD)
Price: 437.00
Your Price:
Availability
-
Full specifications
Quantity
Kit ContentsContains 1 vial of SiteClick Alexa Fluor 647 sDIBO Alkyne for Antibody Labeling in 50 µL DMSO and 1 antibody concentrator
Filter TypeCy5
Chemical ReactivityAzide
Flow Cytometer Laser LinesRed
Labeling MethodClick Chemistry
Labeling Scale1 mg
Shipping ConditionWet Ice
Reactive MoietysDIBO Alkyne
Label or DyeAlexa Fluor™ 647
ColorFar-Red
Product LineAlexa Fluor™, SiteClick
Labeling TargetAzido modified antibodies
Excitation/Emission650/665
Unit Sizefor 1 mg of Ab
Catalog Number
Unit Size
for 5 mg of Ab
Label or Dye
Biotin
Labeling Scale
5 mg
Price (USD)
Price: 656.00
Your Price:
Availability
-
Full specifications
Quantity
Kit ContentsContains 1 vial of SiteClick Biotin sDIBO Alkyne for Antibody Labeling in 250 µL DMSO and 1 antibody concentrator
Chemical ReactivityAzide
Labeling MethodClick Chemistry
Labeling Scale5 mg
Shipping ConditionWet Ice
Reactive MoietysDIBO Alkyne
Label or DyeBiotin
Product LineAlexa Fluor™, SiteClick
Labeling TargetAzido modified antibodies
Unit Sizefor 5 mg of Ab
Showing 10 of 16Show more
Catalog NumberSpecificationsUnit SizeLabel or DyeLabeling ScalePrice (USD)AvailabilityQuantity
S10914Full specifications
For up to 250 µg of AbpHrodo™ Deep Red100-250 µg
Price: 347.00
Your Price:
-
Kit ContentsContains 1 vial of SiteClick pHrodo Deep Red sDIBO Alkyne for Antibody Labeling in 25 µL DMSO, 1 dye removal column, 1 wash vial, 1 collection vial, and PBS exchange buffer
Filter TypeCy5
Chemical ReactivityAzide
Flow Cytometer Laser LinesRed
Labeling MethodClick Chemistry
Labeling Scale100-250 µg
Shipping ConditionWet Ice
Reactive MoietysDIBO Alkyne
Label or DyepHrodo™ Deep Red
ColorDeep Red
Product LinepHrodo™, SiteClick
Labeling TargetAzido modified antibodies
Excitation/Emission640/655
Unit SizeFor up to 250 µg of Ab
C20027Full specifications
1 eachAlexa Fluor™ 488100-250 µg
Price: 274.00
Your Price:
-
Kit ContentsContains 1 vial of SiteClick Alexa Fluor 488 sDIBO Alkyne for Antibody Labeling in 25 µL DMSO and 1 antibody concentrator
Filter TypeGFP
Chemical ReactivityAzide
Flow Cytometer Laser LinesBlue
Labeling MethodClick Chemistry
Labeling Scale100-250 µg
Shipping ConditionWet Ice
Reactive MoietysDIBO Alkyne
Label or DyeAlexa Fluor™ 488
ColorGreen
Product LineAlexa Fluor™, SiteClick
Labeling TargetAzido modified antibodies
Excitation/Emission490/525
Unit Size1 each
C20029Full specifications
1 eachAlexa Fluor™ 647100-250 µg
Price: 274.00
Your Price:
-
Kit ContentsContains 1 vial of SiteClick Alexa Fluor 647 sDIBO Alkyne for Antibody Labeling in 25 µL DMSO and 1 antibody concentrator
Filter TypeCy5
Chemical ReactivityAzide
Flow Cytometer Laser LinesRed
Labeling MethodClick Chemistry
Labeling Scale100-250 µg
Shipping ConditionWet Ice
Reactive MoietysDIBO Alkyne
Label or DyeAlexa Fluor™ 647
ColorFar-Red
Product LineAlexa Fluor™, SiteClick
Labeling TargetAzido modified antibodies
Excitation/Emission650/665
Unit Size1 each
C20031Full specifications
1 eachPrimary Amine100-250 µg
Price: 173.00
Your Price:
-
Kit ContentsContains 1 vial of SiteClick Amine sDIBO Alkyne for Antibody Labeling in 25 µL DMSO and 1 antibody concentrator
Chemical ReactivityAzide
Labeling MethodClick Chemistry
Labeling Scale100-250 µg
Shipping ConditionWet Ice
Reactive MoietysDIBO Alkyne
Label or DyePrimary Amine
Product LineSiteClick
Labeling TargetAzido modified antibodies
Unit Size1 each
C20034Full specifications
1 kitpHrodo™ iFL Red100-250 µg
Price: 308.00
Your Price:
-
Kit ContentsContains 1 vial of SiteClick pHrodo iFL Red sDIBO Alkyne for Antibody Labeling, 1 vial of DMSO, and 1 antibody concentrator
Filter TypeRFP, Texas Red
Chemical ReactivityAzide
Flow Cytometer Laser LinesBlue, Yellow
Labeling MethodClick Chemistry
Labeling Scale100-250 µg
Shipping ConditionWet Ice
Reactive MoietysDIBO Alkyne
Label or DyepHrodo™ iFL Red
ColorRed
Product LinepHrodo™, SiteClick
Labeling TargetAzido modified antibodies
Excitation/Emission560/585
Unit Size1 kit
S10902Full specifications
for 1 mg of AbBiotin1 mg
Price: 309.00
Your Price:
-
Kit ContentsContains 1 vial of SiteClick Biotin sDIBO Alkyne for Antibody Labeling in 50 µL DMSO and 1 antibody concentrator
Chemical ReactivityAzide
Labeling MethodClick Chemistry
Labeling Scale1 mg
Shipping ConditionWet Ice
Reactive MoietysDIBO Alkyne
Label or DyeBiotin
Product LineAlexa Fluor™, SiteClick
Labeling TargetAzido modified antibodies
Unit Sizefor 1 mg of Ab
S10903Full specifications
for 1 mg of AbpHrodo™ iFL Red1 mg
Price: 524.00
Your Price:
-
Kit ContentsContains 1 vial of SiteClick pHrodo iFL Red sDIBO Alkyne for Antibody Labeling, 1 vial of DMSO, and 1 antibody concentrator
Filter TypeRFP, Texas Red
Chemical ReactivityAzide
Flow Cytometer Laser LinesBlue, Yellow
Labeling MethodClick Chemistry
Labeling Scale1 mg
Shipping ConditionWet Ice
Reactive MoietysDIBO Alkyne
Label or DyepHrodo™ iFL Red
ColorRed
Product LinepHrodo™, SiteClick
Labeling TargetAzido modified antibodies
Excitation/Emission560/585
Unit Sizefor 1 mg of Ab
S10904Full specifications
for 1 mg of AbAlexa Fluor™ 4881 mg
Price: 428.00
Your Price:
-
Kit ContentsContains 1 vial of SiteClick Alexa Fluor 488 sDIBO Alkyne for Antibody Labeling in 50 µL DMSO and 1 antibody concentrator
Filter TypeGFP
Chemical ReactivityAzide
Flow Cytometer Laser LinesBlue
Labeling MethodClick Chemistry
Labeling Scale1 mg
Shipping ConditionWet Ice
Reactive MoietysDIBO Alkyne
Label or DyeAlexa Fluor™ 488
ColorGreen
Product LineAlexa Fluor™, SiteClick
Labeling TargetAzido modified antibodies
Excitation/Emission490/525
Unit Sizefor 1 mg of Ab
S10906Full specifications
for 1 mg of AbAlexa Fluor™ 6471 mg
Price: 437.00
Your Price:
-
Kit ContentsContains 1 vial of SiteClick Alexa Fluor 647 sDIBO Alkyne for Antibody Labeling in 50 µL DMSO and 1 antibody concentrator
Filter TypeCy5
Chemical ReactivityAzide
Flow Cytometer Laser LinesRed
Labeling MethodClick Chemistry
Labeling Scale1 mg
Shipping ConditionWet Ice
Reactive MoietysDIBO Alkyne
Label or DyeAlexa Fluor™ 647
ColorFar-Red
Product LineAlexa Fluor™, SiteClick
Labeling TargetAzido modified antibodies
Excitation/Emission650/665
Unit Sizefor 1 mg of Ab
S10907Full specifications
for 5 mg of AbBiotin5 mg
Price: 656.00
Your Price:
-
Kit ContentsContains 1 vial of SiteClick Biotin sDIBO Alkyne for Antibody Labeling in 250 µL DMSO and 1 antibody concentrator
Chemical ReactivityAzide
Labeling MethodClick Chemistry
Labeling Scale5 mg
Shipping ConditionWet Ice
Reactive MoietysDIBO Alkyne
Label or DyeBiotin
Product LineAlexa Fluor™, SiteClick
Labeling TargetAzido modified antibodies
Unit Sizefor 5 mg of Ab
Showing 10 of 16Show more
Create your own covalently labeled antibody conjugates using the SiteClick sDIBO Alkyne labels, which are intended for use with azido-modified antibodies. SiteClick sDIBO Alkyne Antibody Labeling Kits include the sDIBO Alkyne and the antibody concentrator, enabling copper-free covalent attachment of Alexa Fluor and pHrodo dyes, biotin, or molecules of your choice. When used with the SiteClick Antibody Azido Modification Kit (available separately as Cat. No. S20026, S10900, S10901), labeling occurs on the heavy chain N-linked glycans, far away from the antigen-binding domain, enabling excellent reproducibility between separate labeling reactions and multiple antibody species and subtypes. The SiteClick sDIBO alkyne kits for antibody labeling are optimized for conjugation reactions, covalently linking 100 µg, 1 mg, or 5 mg of azide-modified IgG antibody.

The SiteClick Antibody Azido Modification Kit (Cat. No. S20026, S10900, S10901) is recommended for the attachment of an azide moiety to the heavy chains of an unlabeled IgG antibody. Other engineered antibodies containing azido moieties are also suitable.

When used in conjunction with the SiteClick Antibody Azido Modification Kit, the SiteClick sDIBO alkyne kits for antibody labeling give you the option of trying different fluorescent labels for your antibody, attaching other molecules via streptavidin, or attaching your own molecule via amine-reactive moieties, depending on your assay. Our sDIBO alkynes are improved versions of DIBO cyclooctynes, yielding conjugates that are less 'sticky' and emit lower signal background in biological samples.

Contents & Storage

Store in refrigerator (2°C to 8°C) and protect from light.