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Citations & References (9)
Gibco™
Advanced DMEM
Advanced DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation.
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| Catalog Number | Quantity |
|---|---|
| 12491015 | 500 mL |
| 12491023 | 10 x 500 mL |
Catalog number 12491015
Price (TWD)
1,290.00
Online offer
Ends: 30-Sep-2026
1,720.00Save 430.00 (25%)
Each
Quantity:
500 mL
Price (TWD)
1,290.00
Online offer
Ends: 30-Sep-2026
1,720.00Save 430.00 (25%)
Each
Advanced DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM, serum supplementation can be reduced by 50–90% with no change in growth rate or morphology. Cells successfully cultured in Advanced DMEM, with no adaptation, include MDBK, HepG2, COS-7, A549, MDCK, WI-38, and Vero.
This Advanced DMEM is manufactured as follows:
With: High Glucose, Non-essential Amino Acids, Sodium Pyruvate, Phenol Red
Without: L-glutamine
The complete formulation is available.
Using Advanced DMEM
Advanced DMEM is unique from other media due to the addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride. Advanced DMEM requires supplementation with 1–5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement. Many cell lines do not require adaptation to this media. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell LineMDBK, HepG2, COS-7, A549, MDCK, WI-38, and Vero
Concentration1 X
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco
Product TypeDMEM (Dulbecco's Modified Eagle Medium)
Quantity500 mL
Shelf Life12 Months From Date of Manufacture
Shipping ConditionRoom Temperature
ClassificationAnimal Origin
FormLiquid
Serum LevelReduced Serum
SterilitySterile-filtered
Sterilization MethodSterile-filtered
With AdditivesHigh Glucose, Phenol Red, Sodium Pyruvate, Non Essential Amino Acids (NEAA)
Without AdditivesNo Glutamine, No HEPES
Unit SizeEach
Contents & Storage
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Frequently asked questions (FAQs)
What kind of ascorbic acid is present in Advanced DMEM (Cat. No. 12491015 or 12491023)?
What is the manganese concentration in DMEM? Do you offer manganese-free DMEM?
What is the difference between Advanced DMEM Medium and other DMEM formulations?
How long can I keep my media after supplementing with serum?
My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?
Citations & References (9)
Citations & References
Abstract
Viral complementation allows HIV-1 replication without integration.
Journal:Retrovirology
PubMed ID:18613957
BACKGROUND: The integration of HIV-1 DNA into cellular chromatin is required for high levels of viral gene expression and for the production of new virions. However, the majority of HIV-1 DNA remains unintegrated and is generally considered a replicative dead-end. A limited amount of early gene expression from unintegrated DNA
An in vivo model of human small intestine using pluripotent stem cells.
Journal:Nature medicine
PubMed ID:25326803
Differentiation of human pluripotent stem cells (hPSCs) into organ-specific subtypes offers an exciting avenue for the study of embryonic development and disease processes, for pharmacologic studies and as a potential resource for therapeutic transplant(1,2). To date, limited in vivo models exist for human intestine, all of which are dependent upon
Serum-free media for the growth of primary bovine myoblasts.
Journal:Cytotechnology
PubMed ID:31884572
The demand for meat is expected to exceed production capacity by livestock in the coming decennia. Therefore, cultured beef might be a viable alternative to traditional livestock-derived beef. One of the problems however is the sustainability of cultured beef through the use of fetal bovine serum. We aimed to identify
The Isolation of Human Glioblastoma Cells: An Optimised Protocol.
Journal:Acta medica academica
PubMed ID:33543625
Objective: The aim of this study was to establish an optimised protocol for glioblastoma (GBM) cell isolation from brain resection samples, with a high yield and low risk for contamination. Methods: Human GBM cells can be obtained following cranial tumour operations. In sterile conditions, the fragments of viable tissue removed
miR-452-5p suppressed the metastasis of Non-small cell lung cancer through regulating Moesin.
Journal:Journal of Cancer
PubMed ID:37497401
Background: Non-small cell lung cancer (NSCLC) is a common malignant tumor, and it is characterized by high mortality. MicroRNA-452-5p (miR-452-5p) and Moesin (MSN) have been proved to be related with regulation of tumors. If miR-452-5p could regulate NSCLC through targeting MSN remain unclear. Methods: TargetScan data and GEPIA databases were