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Citations & References (9)
Gibco™
Advanced DMEM/F-12
Advanced DMEM/F-12 (Dulbecco's Modified Eagle Medium/Ham's F-12) is a widely used basal medium that allows the culture of mammalian cellsRead more
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| Catalog Number | Quantity |
|---|---|
| 12634010 | 500 mL |
| 12634028 | 10 x 500 mL |
Catalog number 12634010
Price (TWD)
1,743.00
Online offer
Ends: 30-Sep-2026
2,490.00Save 747.00 (30%)
Each
Quantity:
500 mL
Price (TWD)
1,743.00
Online offer
Ends: 30-Sep-2026
2,490.00Save 747.00 (30%)
Each
Advanced DMEM/F-12 (Dulbecco's Modified Eagle Medium/Ham's F-12) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM/F-12, serum supplementation can be reduced by 50–90% with no change in growth rate or morphology. Cells successfully cultured in Advanced DMEM/F-12, with no adaptation, include MRC-5, SP2, Vero, WI-38 and Jurkat.
This Advanced DMEM/F-12 is manufactured as follows:
With: Glucose, Non Essential Amino Acids, Sodium pyruvate, Phenol Red
Without: L-glutamine, HEPES
The complete formulation is available.
Using Advanced DMEM/F-12
Advanced DMEM/F-12 is unique from other media due to addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ II lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride. Advanced DMEM/F-12 requires supplementation with 1–5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement. Many cell lines do not require adaptation to this media. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced Advanced DMEM/F-12 uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell LineMRC-5, SP2, Vero, WI-38 and Jurkat
Concentration1 X
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco
Product TypeDMEM (Dulbecco's Modified Eagle Medium)/F-12
Quantity500 mL
Shelf Life12 Months From Date of Manufacture
Shipping ConditionRoom Temperature
ClassificationAnimal Origin
FormLiquid
Serum LevelReduced Serum
SterilitySterile-filtered
Sterilization MethodSterile-filtered
With AdditivesHigh Glucose, Phenol Red, Sodium Pyruvate, Non Essential Amino Acids (NEAA)
Without AdditivesNo Glutamine, No HEPES
Unit SizeEach
Contents & Storage
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Frequently asked questions (FAQs)
Can NSCs generated using PSC Neural Induction Medium be expanded in the PSC Neural Induction Medium or do they need to be in the recommended NSC expansion medium, as stated in the product manual?
Do you offer DMEM/F12 medium without folic acid?
What is the difference between Advanced DMEM/F12 Medium and DMEM/F12 Medium?
How long can I keep my media after supplementing with serum?
My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?
Citations & References (9)
Citations & References
Abstract
Development of a Three-Dimensional Bioengineering Technology to Generate Lung Tissue for Personalized Disease Modeling.
Journal:Stem cells translational medicine
PubMed ID:28191779
Stem cell technologies, especially patient-specific, induced stem cell pluripotency and directed differentiation, hold great promise for changing the landscape of medical therapies. Proper exploitation of these methods may lead to personalized organ transplants, but to regenerate organs, it is necessary to develop methods for assembling differentiated cells into functional, organ-level
Long-term expansion of epithelial organoids from human colon, adenoma, adenocarcinoma, and Barrett's epithelium.
Journal:Gastroenterology
PubMed ID:21889923
Background & aims: We previously established long-term culture conditions under which single crypts or stem cells derived from mouse small intestine expand over long periods. The expanding crypts undergo multiple crypt fission events, simultaneously generating villus-like epithelial domains that contain all differentiated types of cells. We have adapted the culture
Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche.
Journal:Nature
PubMed ID:19329995
The intestinal epithelium is the most rapidly self-renewing tissue in adult mammals. We have recently demonstrated the presence of about six cycling Lgr5(+) stem cells at the bottoms of small-intestinal crypts. Here we describe the establishment of long-term culture conditions under which single crypts undergo multiple crypt fission events, while
Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro.
Journal:Nature
PubMed ID:21151107
Studies in embryonic development have guided successful efforts to direct the differentiation of human embryonic and induced pluripotent stem cells (PSCs) into specific organ cell types in vitro 1,2. For example, human PSCs have been differentiated into monolayer cultures of liver hepatocytes and pancreatic endocrine cells3–6 that have therapeutic efficacy
In vitro generation of human pluripotent stem cell derived lung organoids.
Journal:eLife
PubMed ID:25803487
Recent breakthroughs in 3-dimensional (3D) organoid cultures for many organ systems have led to new physiologically complex in vitro models to study human development and disease. Here, we report the step-wise differentiation of human pluripotent stem cells (hPSCs) (embryonic and induced) into lung organoids. By manipulating developmental signaling pathways hPSCs