SYBR™ Select Master Mix for CFX
SYBR™ Select Master Mix for CFX
Actual product may vary
SYBR™ Select Master Mix for CFX
SYBR™ Select Master Mix for CFX
Applied Biosystems™

SYBR™ Select Master Mix for CFX

**********************Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at aRead more
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Catalog NumberQuantity
447295410 x 5 mL
44729371 x 1 mL
44729421 x 5 mL
44729522 x 5 mL
44729535 x 5 mL
Catalog number 4472954
Price (TWD)
-
Quantity:
10 x 5 mL
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Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of SYBR Select Master Mix for CFX and added additional capabilities for your gene expression analysis.

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SYBR™ Select Master Mix for CFX is a superior and cost-effective solution for your real-time PCR applications.

• Specific—minimize primer-dimer and non-specific amplification
• Reproducible and sensitive—consistent amplification across a wide dynamic range
• Bright—contains SYBR™ GreenER™ dye for maximum brightness
• Carry-over contamination control—contains heat-labile UDG
• For use with the Bio-Rad CFX96™ Touch or CFX384™ Touch systems
• Standard and fast cycling parameters

Formulated for Maximum Specificity
SYBR™ Select Master Mix for CFX contains all the components needed for your real-time PCR reactions, except the template and primers, in a convenient 2X concentration premix. The master mix includes AmpliTaq™ DNA Polymerase, UP, a highly purified DNA polymerase with a proprietary hot start mechanism that provides exceptional specificity.

Obtain Reproducible Results Across a Wide Dynamic Range
SYBR™ Select Master Mix for CFX is specially formulated to provide robust results from 100 ng to 0.1 pg cDNA per reaction (Figure 1) and can reliably detect a single copy of genomic DNA (Figure 2).

Contains SYBR™ GreenER™ Dye and Heat-labile UDG
• SYBR™ GreenER™ dye is less inhibitory to PCR than SYBR™ Green I dye, resulting in brighter signals
• Heat-labile uracil-DNA glycosylase (UDG) is included for worry-free carryover contamination control

View performance data.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Equipment)AB 7900HT, Standard Mode, AB StepOne™, Fast Mode, AB 7500, Standard Mode, AB 7500, Fast Mode, Bio-Rad CFX96 Touch™, AB StepOnePlus™, Fast Mode, AB 7900HT, Fast Mode, AB StepOne™, Standard Mode, Bio-Rad CFX Connect™, AB 7900 HT 384-Well System, Bio-Rad CFX384 Touch™, AB StepOnePlus™, Standard Mode
FormatVial
Hot StartBuilt-In Hot Start
No. of Reactions5000 Reactions
Passive Reference DyeROX (Pre-mixed)
PolymeraseAmpliTaq DNA Polymerase
Product LineSYBR
Product TypeReal Time PCR SYBR Master Mix
Purity or Quality GradeUP (Ultra Pure)
Quantity10 x 5 mL
Sample TypeDNA (Genomic), cDNA
Sufficient For5000 Reactions of 20 μL
Volume10 x 5 mL
Concentration2X
Detection MethodSYBR
For Use With (Application)Gene Expression
FormLiquid
GC-Rich PCR PerformanceHigh
Label or DyeSYBR GreenER
PCR MethodqPCR
Reaction SpeedFast or Standard
Unit SizeEach
Contents & Storage
The 2x mix contains SYBR™ GreenER™ dye, AmpliTaq™ DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, and optimized buffer components. Contains 10 X 5 mL bottles, sufficient for 5000 reactions at 20 μL. Store at 2-8™C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

Frequently asked questions (FAQs)

How does the SYBR GreenER Dye Chemistry work?

The SYBR GreenER dye binds to double stranded DNA formed during PCR. During PCR, the polymerase amplifies the target sequence which creates the amplicon. As PCR continues, the dye binds to each new copy of double-stranded DNA, leading to more amplicons being created. The result is an increase in fluorescence intensity proportional to the amount of double-stranded PCR product produced.