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Thermo Scientific™
HotStart-IT Binding Protein
HotStart-IT Binding Protein is the active component in a novel hot start technology called primer sequestration.
| Catalog Number | Quantity |
|---|---|
| 71194400UG also known as 71194 400 UG | 400 μg (200 x 25 μL rxns) |
Catalog number 71194400UG
also known as 71194 400 UG
Price (TWD)
4,662.00
Online offer
Ends: 31-Mar-2026
6,660.00Save 1,998.00 (30%)
Each
Quantity:
400 μg (200 x 25 μL rxns)
Price (TWD)
4,662.00
Online offer
Ends: 31-Mar-2026
6,660.00Save 1,998.00 (30%)
Each
HotStart-IT Binding Protein is the active component in the novel hot start technology known as primer sequestration. In primer sequestration, hot start PCR methods reduce or eliminate non-specific primer-extension products formed at lower temperatures during assembly of PCR reactions. At these less stringent annealing temperatures, primers may bind non-specifically, which often leads to unwanted amplification products and primer-dimers. To resolve this problem, we have produced a high-quality DNA binding protein that is especially useful at sequestering primers at lower temperatures, making them unavailable for use by a polymerase (see figure).
Features
- Room temperature reaction setup
- Minimizes amplification of non-specific products and primer-dimers (see figure)
- Ideal for complex templates and multiplex reactions
- No extensive heating step required
- Free from detectable non-specific nucleases
- Technology is portable to a polymerase of choice
This primer-sequestration technique effectively blocks DNA synthesis from mis-priming events at lower temperatures. Following the initial denaturation step during PCR, the protein is inactivated, and the primers are free to participate in the amplification reaction.
HotStart-IT Binding Protein performs well in many standard PCR reaction buffers and has been designed for PCR applications that demand high specificity and sensitivity. PCR with Taq DNA Polymerase and HotStart-IT Binding Protein shifts production of primer-dimers to a specific target of 306 bp from 1 ng of human genomic DNA relative to Taq DNA Polymerase alone.
Application
- Hot-Start PCR amplification
Notes
- One microgram of HotStart-IT Binding Protein sequesters about 5 pmol of primers.
- HotStart-IT Binding Protein is sourced from recombinant protein expressed in E. coli.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Compatible Buffer1X PCR Reaction Buffer
For Use With (Application)Hot-start PCR
Product TypeHotStart-IT Binding Protein
Quantity400 μg (200 x 25 μL rxns)
Shipping ConditionDry Ice
Unit SizeEach
Contents & Storage
• HotStart-IT Binding Protein, 400 μg
Store at -20°C.
Store at -20°C.