Halt™ Protease and Phosphatase Inhibitor Cocktail (100X)
Halt™ Protease and Phosphatase Inhibitor Cocktail (100X)
Citations & References (7)
Thermo Scientific™

Halt™ Protease and Phosphatase Inhibitor Cocktail (100X)

Thermo Scientific Halt Protease and Phosphatase Inhibitor Cocktail (100X) provides the convenience of a single solution with full protein sampleRead more
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Catalog NumberQuantity
784401 mL
7844224 x 100 μL
7844610 mL
784445 x 1 mL
Catalog number 78440
Price (TWD)
11,780.00
Online offer
Ends: 31-Dec-2025
15,700.00
Save 3,920.00 (25%)
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Quantity:
1 mL
Request bulk or custom format
Price (TWD)
11,780.00
Online offer
Ends: 31-Dec-2025
15,700.00
Save 3,920.00 (25%)
Each
Add to cart
Thermo Scientific Halt Protease and Phosphatase Inhibitor Cocktail (100X) provides the convenience of a single solution with full protein sample protection for cell and tissue lysates.

Features of Halt Protease and Phosphatase Inhibitor Cocktail (100X):

• Complete protection—all-in-one cocktail contains both protease and phosphatase inhibitors
• Multiple package sizes—choose from four convenient packages sizes to match nearly any scale
• Convenient —the 100X liquid formulation is much easier and more effective to use than individual inhibitors and other formats
• Easy storage—aqueous-based format allows for convenient cold-room storage without freezing, by contrast with DMSO-based cocktails
• Compatible—can be used in mass spectrometry (MS) because it does not contain AEBSF, which can cause peaks to shift
• Validated—fully compatible with Thermo Scientific Pierce Cell Lysis Buffers

Halt Protease and Phosphatase Inhibitor Cocktail is specifically optimized to protect proteins from degradation during extraction and purification. The cocktail contains inhibitors against the major classes of proteases and phosphatases, targeting aminopeptidases, cysteine and serine proteases and serine/threonine and protein tyrosine phosphatases. To promote the inhibition of metalloproteases, 0.5M EDTA is provided in a separate tube.

Related Products
Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X)
For Research Use Only. Not for use in diagnostic procedures.
Specifications
BufferLysis Buffer
InhibitorsProtease Inhibitor, Phosphatase Inhibitor
Quantity1 mL
Reagent TypeProtease Inhibitor, Phosphatase Inhibitor
FormLiquid
Product LineHalt
Product TypeProtease and Phosphatase Inhibitor Cocktail
Unit SizeEach
Contents & Storage
Upon receipt store at 4°C

Frequently asked questions (FAQs)

Can I store the 1X solution of the Halt Protease and Phosphatase Inhbitor Cocktail and if so, for how long?

The stability of the 1X solution is undetermined. For best results, prepare solutions just before use. The 100X concentrate is stable for at least one year stored at 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What are the concentrations of the various components of the combined Halt Protease and Phosphatase Inhibitor Cocktail?

Although we reveal the identity of the inhibitors in the combined cocktail, their concentrations are proprietary.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Do you offer the components of the Halt Protease and Phosphatase Cocktail individually?

Yes. Halt Protease, Halt Phosphatase and Protease/Phosphatase combined Inhibitor Cocktails are available. In addition, some of the individual inhibitors of the Halt Protease and Phosphatase Inhibitor Cocktail are offered separately.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

When do I need to add EDTA when using the Halt Protease and Phosphatase Cocktail?

EDTA chelates the metal ions required for metalloprotease activity. Add EDTA if metalloproteases are likely to adversely affect target protein function; however, avoid using EDTA when the downstream application involves metal chelate affinity purification or the target protein requires metals for proper function.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How much of the Halt Protease and Phosphatase Cocktail do I use for my sample?

The Halt Protease and Phosphatase Cocktail is supplied at a 100X concentrate and is used at 1X. For example, add 10µL of 100X Halt Cocktail to 1mL of sample. Some samples, however, contain high levels of proteases or phosphatase and might require a more concentrated treatment (i.e., 2-3X).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

View all Halt™ Protease and Phosphatase Inhibitor Cocktail (100X), 1 mL FAQs

Citations & References (7)

Citations & References
Abstract
Deglycosylation of pathological specimens alters performance of diagnostic PDL1 antibodies.
Authors:Dressler FF,Dabadghao DS,Klapper L,Perner S,Idel C,Ribbat-Idel J
Journal:Virchows Archiv : an international journal of pathology
PubMed ID:35779078
Immunohistochemical (IHC) predictive quantitation of PDL1 expression is obligatory in many cancer entities with improved response to immune checkpoint inhibition in PDL1-positive subgroups. With recent demonstration of increased positivity rates after enzymatic deglycosylation in breast cancer specimens, a comparative analysis with two different antibodies and extended controls was performed in ... More
Imlunestrant Is an Oral, Brain-Penetrant Selective Estrogen Receptor Degrader with Potent Antitumor Activity in ESR1 Wild-Type and Mutant Breast Cancer.
Authors:Bhagwat SV,Mur C,Vandekopple M,Zhao B,Shen W,Marugán C,Capen A,Kindler L,Stephens JR,Huber L,Castanares MA,Garcia-Tapia D,Cohen JD,Bastian J,Mattioni B,Yuen E,Baker TK,Rodriguez Cruz V,Fei D,Manro JR,Pulliam N,Dowless MS,Ortiz Ruiz MJ,Yu C,Puca L,Klippel A,Bacchion F,Ismail-Khan R,Rodrik-Outmezguine V,Peng SB,Lallena MJ,Gong X,de Dios A
Journal:Cancer research
PubMed ID:39652577
Targeting of the estrogen receptor (ER) by antiestrogens is the standard of care for patients with ER+ HER2- advanced/metastatic breast cancer. Although antiestrogens that degrade ERα (fulvestrant) or block estrogen production (aromatase inhibitors) have improved patient outcomes, clinically important challenges remain related to drug administration, limited bioavailability, lack of brain ... More
Urologic pharmacology, Part I.
Authors:
Journal:Seminars in urology
PubMed ID:3843699
hCLCA1 and mCLCA3 are secreted non-integral membrane proteins and therefore are not ion channels.
Authors:Gibson A, Lewis AP, Affleck K, Aitken AJ, Meldrum E, Thompson N
Journal:J Biol Chem
PubMed ID:15919655
'Proteins of the CLCA gene family have been proposed to mediate calcium-activated chloride currents. In this study, we used detailed bioinformatics analysis and found that no transmembrane domains are predicted in hCLCA1 or mCLCA3 (Gob-5). Further analysis suggested that they are globular proteins containing domains that are likely to be ... More
Quantitative PCR analysis of DNA, RNAs, and proteins in the same single cell.
Authors:Ståhlberg A, Thomsen C, Ruff D, Åman P
Journal:Clin Chem
PubMed ID:23014600
The single cell represents the basic unit of all organisms. Most investigations have been performed on large cell populations, but understanding cell dynamics and heterogeneity requires single-cell analysis. Current methods for single-cell analysis generally can detect only one class of analytes. ... More
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