Jump-In™ Fast Gateway™ Core Kit

The Jump-In™ Fast Gateway™ Core Kit is intended for rapid engineering of mammalian cell lines through the stable and irreversibleRead more

Catalog Number	Quantity
A10894	1 Kit

Catalog number A10894

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1 Kit

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The Jump-In™ Fast Gateway™ Core Kit is intended for rapid engineering of mammalian cell lines through the stable and irreversible integration of your gene of interest (GOI) at specific genomic loci (pseudo attP sites). Following stable integration, only 5-10 clones need to be analyzed to identify clones with high expression levels.
The Jump-In™ Fast Gateway™ Core Kit contains the promoter-less pJTI™ Fast DEST expression vector and a vector for transient expression of the PhiC31 integrase.
Please note: the pJTI™ Fast DEST expression vector requires the cloning of a promoter upstream of the gene of interest, which is facilitated by any of the MultiSite Gateway™ Pro Kits.
This kit is also a component of the Jump-In™ Fast Gateway™ System.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cloning MethodGateway™

Delivery TypeTransfection

Expression SystemMammalian

For Use With (Application)Transfection

High-throughput CompatibilityHigh-throughput Compatible

Key FunctionsStable Cell Line Development, Targeted Integration, Clone your own Promoter

Product LineJump-In

Product TypeCore Kit

Protein TagUntagged

Quantity1 Kit

Selection Agent (Eukaryotic)Hygromycin

Selection Marker EukaryoticHygroR

Cell TypeMammalian

FormatKit

PromoterNone (Promoterless)

VectorJump-In Vectors, pJTI

Unit SizeEach

Contents & Storage

The Jump-In™ Fast Gateway™ Core Kit is composed of 2 vectors:
pJTI™ Fast DEST Vector (150 ng⁄μl; total 40 μl),
pJTI™ PhiC31 Int Vector (0.5 μg⁄μl; total 20 μl).

Store vectors at -20°C.

Frequently asked questions (FAQs)

Can I perform the single-step protocol for the BP/LR Clonase reaction using BP Clonase enzyme and LR Clonase enzyme instead of BP Clonase II enzyme and LR Clonase II enzyme?

In the single-step protocol for the BP/LR Clonase reaction, we would not recommend substituting the BP Clonase II/LR Clonase II enzymes with BP Clonase /LR Clonase enzymes as this would result in very low recombination efficiency.

Do you have a recommended single-step protocol for BP/LR recombination?

Yes, we have come up with a single-step protocol for BP/LR Clonase reaction (http://www.thermofisher.com/us/en/home/life-science/cloning/gateway-cloning.html#1), where DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money.

How can I move my gene of interest from a Gateway-adapted expression clone to a new Destination vector as I have lost the entry clone?

We would recommend performing a BP reaction with a Donor vector in order to obtain an entry clone. This entry clone can then be used in an LR reaction with the Destination vector to obtain the new expression clone.

Can I purchase the 5X LR Clonase buffer or 5X BP Clonase buffer separately?

We do not offer the 5X LR Clonase buffer and 5X BP Clonase buffer as standalone products. They are available as part of the enzyme kits.

Do you offer Gateway vectors for expression in plants?

We do not offer any Gateway vectors for expression in plants.

View all Jump-In™ Fast Gateway™ Core Kit FAQs