Citations & References (10)

Invitrogen™

Amplex™ Red Monoamine Oxidase Assay Kit

Ideal for enzyme and protein activity assays

Catalog Number	Quantity
A12214	500 Assays

Catalog number A12214

Price (TWD)

Quantity:

500 Assays

Price (TWD)

The Amplex Red Monoamine Oxidase Assay Kit provides a sensitive and simple fluorometric method for detecting monoamine oxidase activity in purified enzyme preparations at levels as low as 12 μU/mL and in tissue samples with as little as 20 μg protein in a 100 μL assay volume.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection MethodFluorescence

Dye TypeAmplex Red

Emission Wavelength585 nm

Excitation Wavelength571 nm

Format96-well Plate, Cuvettes

Quantity500 Assays

Shipping ConditionRoom Temperature

For Use With (Application)Enzyme and Metabolic Activity

For Use With (Equipment)Fluorometer, Microplate Reader

Product LineAmplex

Product TypeAmplex Red Assay Kit

Unit SizeEach

Contents & Storage

Store in freezer -5°C to -30°C and protect from light.

Frequently asked questions (FAQs)

I'm using an Amplex Red kit, the reagent changes color to pink almost immediately in my own Krebs-Ringer buffer but not in HBSS. Why is this?

The components of Krebs-Ringer buffer (salts) should not cause oxidation of the Amplex reagent (which, in the presence of peroxidase and H₂O₂ oxidizes to resorufin, which is pink in color and fluorescent). Try water alone (the water used to make the Krebs-Ringer buffer). Since Hank's Buffered Saline Solution is typically purchased rather than made in the lab, it likely would not have the same contaminant. Another option is to degas the buffer prior to use to removed dissolved oxygen radicals.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can Amplex Red Assays be performed using cell lysates?

This is not recommended. The presence of endogenous proteases can complicate the assay by degrading the horseradish peroxidase (HRP). Endogenous peroxidases and antioxidants can modify the H₂O₂ required for the reaction, competing with HRP (and catalase) for the substrate.

The Amplex Red Assays are best performed with either purified enzymes or extracted H₂O₂ in a defined buffer system, extracellular solutions or body fluids (media, serum, etc.) that do not exhibit high levels of endogenous protease or oxidase activity and do not contain antioxidants.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (10)

Citations & References

Abstract

High-throughput screening for monoamine oxidase-A and monoamine oxidase-B inhibitors using one-step fluorescence assay.

Authors:Guang HM, Du GH

Journal:Acta Pharmacol Sin

PubMed ID:16723097

'AIM: To develop high-throughput screening (HTS) assays for monoamine oxidase (MAO)-A and MAO-B inhibitors. METHODS: A fluorescence probe based method measuring MAO-A and MAO-B activity was established and optimized, with its sensitivity, stability and specificity evaluated. Reaction conditions including enzyme sources, substrate concentrations, incubation volume and reaction time in 384-well ... More

Catecholamines regulate the activity, secretion, and synthesis of renalase.

Authors:Li G, Xu J, Wang P, Velazquez H, Li Y, Wu Y, Desir GV,

Journal:Circulation

PubMed ID:18299506

'BACKGROUND: We previously identified renalase, a secreted novel amine oxidase that specifically degrades circulating catecholamines. Parenteral administration of either native or recombinant renalase lowers blood pressure, heart rate, and cardiac contractility by metabolizing circulating catecholamines. Renalase plasma levels are markedly reduced in patients with chronic kidney disease. It is not ... More

The PPARgamma agonist pioglitazone is effective in the MPTP mouse model of Parkinson's disease through inhibition of monoamine oxidase B.

Authors:Quinn LP, Crook B, Hows ME, Vidgeon-Hart M, Chapman H, Upton N, Medhurst AD, Virley DJ,

Journal:Br J Pharmacol

PubMed ID:18332857

'BACKGROUND AND PURPOSE: The peroxisome proliferator-activated receptor-gamma (PPARgamma) agonist pioglitazone has previously been shown to attenuate dopaminergic cell loss in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) mouse model of Parkinson''s disease, an effect attributed to its anti-inflammatory properties. In the present investigation, we provide evidence that pioglitazone is effective in the MPTP mouse ... More

Renalase lowers ambulatory blood pressure by metabolizing circulating adrenaline.

Authors:Desir GV, Tang L, Wang P, Li G, Sampaio-Maia B, Quelhas-Santos J, Pestana M, Velazquez H,

Journal:J Am Heart Assoc

PubMed ID:23130169

'Blood pressure is acutely regulated by the sympathetic nervous system through the action of vasoactive hormones such as epinephrine, norepinephrine, and dopamine. Renalase, a recently described, secreted flavoprotein, acutely decreases systemic pressure when administered in vivo. Single-nucleotide polymorphisms present in the gene are associated with hypertension, cardiac disease, and diabetes. ... More

Quantitative structure-activity relationship and complex network approach to monoamine oxidase A and B inhibitors.

Authors:Santana L, González-Díaz H, Quezada E, Uriarte E, Yáñez M, Viña D, Orallo F,

Journal:J Med Chem

PubMed ID:18834112

'The work provides a new model for the prediction of the MAO-A and -B inhibitor activity by the use of combined complex networks and QSAR methodologies. On the basis of the obtained model, we prepared and assayed 33 coumarin derivatives, and the theoretical prediction was compared with the experimental activity ... More

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