CytoTune™-iPS 2.0 Sendai Reprogramming Kit
CytoTune™-iPS 2.0 Sendai Reprogramming Kit
Citations & References (1)
Invitrogen™

CytoTune™-iPS 2.0 Sendai Reprogramming Kit

The CytoTune-iPS 2.0 Sendai Reprogramming Kit improves upon the well-referenced CytoTune-iPS Sendai Reprogramming Kit.
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Catalog NumberQuantity
A165171 Ea.
A165183 Pk.
Catalog number A16517
Price (TWD)
-
Quantity:
1 Ea.

The CytoTune-iPS 2.0 Sendai Reprogramming Kit improves upon the well-referenced CytoTune-iPS Sendai Reprogramming Kit. This system continues to be easy to use and works with many different somatic cell types, using Sendai particles to deliver Yamanaka factors, which have been shown to be critical for efficient generation of iPSCs. This kit includes three vector preparations: polycistronic Klf4–Oct3/4–Sox2, cMyc, and Klf4.

Features include:

  • Ease of use— still requires only one application of the virus to obtain successful reprogramming
  • Increased efficiency—usually at least two-fold higher reprogramming efficiencies than the original kit
  • Rapid clearance—use the non-integrating Sendai virus that remains in the cytoplasm, reducing the likelihood of integration into the host genome. With the CytoTune-iPS 2.0 Sendai virus, the Sendai backbone and transgenes clear the cytoplasm faster than the original.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Delivery TypeSendai virus
For Use With (Application)Stem Cell Engineering & Reprogramming
Product LineCytoTune
Product TypeSendai Reprogramming Kit
PromoterProprietary
Quantity1 Ea.
Shipping ConditionDry Ice
Sub Cellular LocalizationCytoplasm
Cell TypeiPSC
FormatKit
Unit SizeEach
Contents & Storage
3 Tubes, store (at -68°C to -85°C.)

Frequently asked questions (FAQs)

I am interested in using StemFlex Medium in somatic cell reprogramming. What protocol should I follow for human fibroblasts?

We have evaluated the use of Cytotune-iPS 2.0 Sendai Reprogramming Kit (Cat. No. A16517) for somatic cell reprogramming of both neonatal and adult human dermal fibroblasts. For fibroblasts, follow the instructions provided in the Cytotune 2.0 reprogramming manual for feeder-free reprogramming (pgs. 16-20). On Day 7, you may use rhVTN-N (Cat. No. A400457 or A400458), Geltrex Flex matrix (Cat. No. A4000046801, A4000046802, or A4000046803), or rhLaminin-521 (Cat. No. A29248 or A29249). From Day 8 onward, rather than feeding daily with Essential 8 Medium, we recommend that you feed reprogrammed fibroblasts every-other-day with StemFlex Medium.

I am interested in using StemFlex Medium in somatic cell reprogramming. What protocol should I follow for blood cells?

We have evaluated the use of Cytotune-iPS 2.0 Sendai Reprogramming Kit (Cat. No. A16517) for somatic cell reprogramming of CD34+ blood cells. For CD34+ cells, follow the instructions provided in the Cytotune 2.0 reprogramming manual for feeder-free reprogramming (pgs. 39-44). On Day 3, you can utilize rhVTN-N (Cat. No. A400457 or A400458), Geltrex Flex matrix (Cat. No. A4000046801, A4000046802, or A4000046803), or rhLaminin-521 (Cat. No. A29248 or A29249). From Day 8 onward, rather than feeding daily with Essential 8 Medium, reprogrammed CD34+ cells should be fed every-other-day with StemFlex Medium.

What is the shelf life of the CytoTune-iPS 2.0 Sendai Reprogramming Kit (Cat. No. A16517)?

The shelf life for this kit is 3 years from the date of manufacture. Both the date of manufacture and the date of expiration are stated on the lot-specific Certificate of Analysis (COA).

Find additional tips, troubleshooting help, and resources within our Stem Cell Culture Support Center.

What is the shelf life of the CytoTune-iPS 2.0 Sendai Reprogramming Kit?

The shelf life for the CytoTune-iPS 2.0 Sendai Reprogramming Kit is 2 years when stored at -80 degrees C with no repeated freeze-thaw cycles.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the optimal passage number for reprogramming patient fibroblasts using the CytoTune-iPS 2.0 Sendai Reprogramming Kit?

We recommend reprogramming patient cells at the earliest passage possible. However, it is important to have the cells growing and healthy, which can take between 1-4 weeks. The cells are usually ready to reprogram once they have gone through a total of 3-4 passages.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all CytoTune™-iPS 2.0 Sendai Reprogramming Kit FAQs

Citations & References (1)

Citations & References
Abstract
Whole-genome mutational burden analysis of three pluripotency induction methods.
Authors:Bhutani K, Nazor KL, Williams R, Tran H, Dai H, Džakula Ž, Cho EH, Pang AW, Rao M, Cao H, Schork NJ, Loring JF,
Journal:Nat Commun
PubMed ID:26892726
'There is concern that the stresses of inducing pluripotency may lead to deleterious DNA mutations in induced pluripotent stem cell (iPSC) lines, which would compromise their use for cell therapies. Here we report comparative genomic analysis of nine isogenic iPSC lines generated using three reprogramming methods: integrating retroviral vectors, non-integrating ... More