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Citations & References (6)
Invitrogen™
Amplex™ Red Uric Acid/Uricase Assay Kit
The Amplex™ Red Uric Acid/Uricase Assay Kit provides a sensitive and simple method for detecting uric acid or uricase usingRead more
| Catalog Number | Quantity |
|---|---|
| A22181 | 400 Assays |
Catalog number A22181
Price (TWD)
15,190.00
線上優惠
Ends: 31-Dec-2025
21,700.00Save 6,510.00 (30%)
Each
Quantity:
400 Assays
Price (TWD)
15,190.00
線上優惠
Ends: 31-Dec-2025
21,700.00Save 6,510.00 (30%)
Each
The Amplex™ Red Uric Acid/Uricase Assay Kit provides a sensitive and simple method for detecting uric acid or uricase using either a fluorescence microplate reader or fluorometer.
See our complete line of Fluorescence Microplate assays.
• Detect concentrations as low as 100 nM uric acid or 0.2 mU/mL of uricase activity
• Format allows for multiple time point measurements
• Designed for minimal autofluorescence interference
Serum uric acid is the end product of purine metabolism in the body tissues and is cleared through the kidneys by glomerular filtration. Most animals can metabolize uric acid to more readily excreted products, but humans lack the necessary enzyme, urate oxidase (uricase), as a result of the presence of two “nonsense mutations” in the human gene for uricase.
The Amplex™ Red Uric Acid/Uricase Assay Kit provides an ultrasensitive method for detecting uric acid or for monitoring uricase activity. In the assay, uricase catalyzes the conversion of uric acid to allantoin, hydrogen peroxide, and carbon dioxide. In the presence of horseradish peroxidase (HRP), hydrogen peroxide reacts stoichiometrically with the Amplex™ Red reagent to generate the red-fluorescent oxidation product resorufin.
Because resorufin has absorption and fluorescence emission maxima of approximately 571 nm and 585 nm, respectively, there is little interference from autofluorescence in most biological samples.
Use Amplex™ Red Assays for a Broad Range of Investigations
A wide variety of validated Amplex™ Red assays are available for studying cell signaling and lipids, neurobiology, inflammation and immune function, and metabolism. We also offer Amplex™ UltraRed Reagent (Cat. No. A36006), a second-generation reagent providing greater sensitivity and brighter fluorescence, and the Amplex™ Red/UltraRed Stop Reagent (Cat. No. A33855). The Amplex™ Red/UltraRed Stop Reagent provides convenience and control by allowing the fluorescence signal-generating reaction to be terminated at a user-determined time point. After addition of the stop reagent, the fluorescence signal remains stable for at least three hours. Custom assay design and packaging are also available.
See our complete line of Fluorescence Microplate assays.
• Detect concentrations as low as 100 nM uric acid or 0.2 mU/mL of uricase activity
• Format allows for multiple time point measurements
• Designed for minimal autofluorescence interference
Serum uric acid is the end product of purine metabolism in the body tissues and is cleared through the kidneys by glomerular filtration. Most animals can metabolize uric acid to more readily excreted products, but humans lack the necessary enzyme, urate oxidase (uricase), as a result of the presence of two “nonsense mutations” in the human gene for uricase.
The Amplex™ Red Uric Acid/Uricase Assay Kit provides an ultrasensitive method for detecting uric acid or for monitoring uricase activity. In the assay, uricase catalyzes the conversion of uric acid to allantoin, hydrogen peroxide, and carbon dioxide. In the presence of horseradish peroxidase (HRP), hydrogen peroxide reacts stoichiometrically with the Amplex™ Red reagent to generate the red-fluorescent oxidation product resorufin.
Because resorufin has absorption and fluorescence emission maxima of approximately 571 nm and 585 nm, respectively, there is little interference from autofluorescence in most biological samples.
Use Amplex™ Red Assays for a Broad Range of Investigations
A wide variety of validated Amplex™ Red assays are available for studying cell signaling and lipids, neurobiology, inflammation and immune function, and metabolism. We also offer Amplex™ UltraRed Reagent (Cat. No. A36006), a second-generation reagent providing greater sensitivity and brighter fluorescence, and the Amplex™ Red/UltraRed Stop Reagent (Cat. No. A33855). The Amplex™ Red/UltraRed Stop Reagent provides convenience and control by allowing the fluorescence signal-generating reaction to be terminated at a user-determined time point. After addition of the stop reagent, the fluorescence signal remains stable for at least three hours. Custom assay design and packaging are also available.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
Dye TypeOther Label(s) or Dye(s)
FormatTube(s), 96-well plate
Quantity400 Assays
Shipping ConditionRoom Temperature
For Use With (Application)Uric Acid/Uricase Assay
For Use With (Equipment)Microplate Reader, Spectrophotometer, Fluorometer
Product LineAmplex
Product TypeAmplex Red Assay Kit
Unit SizeEach
Contents & Storage
Store in freezer -5°C to -30°C and protect from light.
Frequently asked questions (FAQs)
I'm using an Amplex Red kit, the reagent changes color to pink almost immediately in my own Krebs-Ringer buffer but not in HBSS. Why is this?
Can Amplex Red Assays be performed using cell lysates?
Citations & References (6)
Citations & References
Abstract
Use of colorimetric test strips for monitoring the effect of hemodialysis on salivary nitrite and uric acid in patients with end-stage renal disease: a proof of principle.
Journal:Clin Chem
PubMed ID:18676588
'BACKGROUND: Initial screening of potential biomarkers for monitoring dialysis was performed with saliva samples collected from patients with end-stage renal disease (ESRD). A more thorough analysis of the most promising markers identified in the initial screening was conducted with saliva samples acquired at hourly intervals throughout dialysis to monitor analyte
HucR, a novel uric acid-responsive member of the MarR family of transcriptional regulators from Deinococcus radiodurans.
Journal:J Biol Chem
PubMed ID:15448166
'The MarR family of transcriptional regulators comprises a subset of winged helix DNA-binding proteins and includes numerous members that function in environmental surveillance of aromatic compounds. We describe the characterization of HucR, a novel MarR homolog from Deinococcus radiodurans that demonstrates phenolic sensing capabilities. HucR binds as a homodimer to
Fructose induces transketolase flux to promote pancreatic cancer growth.
Journal:Cancer Res
PubMed ID:20647326
'Carbohydrate metabolism via glycolysis and the tricarboxylic acid cycle is pivotal for cancer growth, and increased refined carbohydrate consumption adversely affects cancer survival. Traditionally, glucose and fructose have been considered as interchangeable monosaccharide substrates that are similarly metabolized, and little attention has been given to sugars other than glucose. However,
Activation of NLRP3 inflammasome in alveolar macrophages contributes to mechanical stretch-induced lung inflammation and injury.
Journal:J Immunol
PubMed ID:23436933
Mechanical ventilation of lungs is capable of activating the innate immune system and inducing sterile inflammatory response. The proinflammatory cytokine IL-1ß is among the definitive markers for accurately identifying ventilator-induced lung inflammation. However, mechanisms of IL-1ß release during mechanical ventilation are unknown. In this study, we show that cyclic stretch
IL-1R1/MyD88 signaling is critical for elastase-induced lung inflammation and emphysema.
Journal:J Immunol
PubMed ID:20007584
Lung emphysema and fibrosis are severe complications of chronic obstructive pulmonary disease, and uncontrolled protease activation may be involved in the pathogenesis. Using experimental elastase-induced acute inflammation, we demonstrate here that inflammation and development of emphysema is IL-1R1 and Toll/IL-1R signal transduction adaptor MyD88 dependent; however, TLR recognition is dispensable