Alexa Fluor™ 488 5-TFP (Alexa Fluor™ 488 Carboxylic Acid, 2,3,5,6-Tetrafluorophenyl Ester), 5-isomer
Alexa Fluor™ 488 5-TFP (Alexa Fluor™ 488 Carboxylic Acid, 2,3,5,6-Tetrafluorophenyl Ester), 5-isomer
Citations & References (35)
Invitrogen™

Alexa Fluor™ 488 5-TFP (Alexa Fluor™ 488 Carboxylic Acid, 2,3,5,6-Tetrafluorophenyl Ester), 5-isomer

Tetrafluorophenyl (TFP) esters are an improvement over the succinimidyl ester (SE or NHS-ester) chemistry typically used to attach fluorophores orRead more
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Catalog NumberQuantity
A300051 mg
Catalog number A30005
Price (TWD)
14,000.00
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Ends: 31-Dec-2025
20,000.00
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Quantity:
1 mg
Price (TWD)
14,000.00
Online offer
Ends: 31-Dec-2025
20,000.00
Save 6,000.00 (30%)
Each
Add to cart
Tetrafluorophenyl (TFP) esters are an improvement over the succinimidyl ester (SE or NHS-ester) chemistry typically used to attach fluorophores or haptens to the primary amines of biomolecules. Both reactive chemistries produce the same strong amide bond between the dye or hapten and the compound of interest, but TFP esters are less susceptible to spontaneous hydrolysis during conjugation reactions. Alexa Fluor TFP esters are stable for several hours at the basic pH typically used for reactions – far outlasting succinimidyl esters.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Chemical ReactivityAmine
Label or DyeAlexa Fluor™ 488
Product TypeCarboxylic Acid Tetrafluorophenyl Ester
Quantity1 mg
Reactive MoietyActive Ester, TFP Ester
Shipping ConditionRoom Temperature
Label TypeAlexa Fluor
Product LineAlexa Fluor
Unit SizeEach
Contents & Storage
Store in freezer (-5 to -30°C) and protect from light.

Frequently asked questions (FAQs)

I am labeling a protein with Alexa Fluor 488 SDP ester. The manual recommends using a sodium bicarbonate buffer at pH 8.3. Can I use a different buffer instead?

Yes. The important thing is to use a buffered solution with a pH between 8.0 and 8.5. Do not use Tris buffer, which has amine groups. Most other buffers will work fine in that pH range. This is also true for other amine-reactive dyes, such as succinimidyl (NHS) esters or TFP esters.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am not going to use all of my Alexa Fluor succinimidyl ester reactive dye. Can I just make it up in DMSO and store aliquots at -20 degrees C?

This is not recommended. Any trace amounts of water in the DMSO can promote spontaneous hydrolysis over time. Even if using anhydrous DMSO, DMSO is hygroscopic; it readily absorbs moisture from the atmosphere over time. A better alternative is to dissolve the reactive dye in a volatile solvent, make smaller aliquots and then evaporate off the solvent using a vacuum pump. The smaller aliquots of solid reactive dye should then be stored frozen, desiccated and protected from light. Contact Technical Support by sending an email to techsupport@thermofisher.com for the recommended volatile solvent.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (35)

Citations & References
Abstract
Identification, cloning, and characterization of two N-acetylgalactosamine-binding lectins from the albumen gland of Helix pomatia.
Authors:Markiv A, Peiris D, Curley GP, Odell M, Dwek MV,
Journal:J Biol Chem
PubMed ID:21372134
Helix pomatia agglutinin (HPA), the lectin from the albumen gland of the Roman snail, has been used in histochemical studies relating glycosylation changes to the metastatic potential of solid tumors. To facilitate the use of HPA in a clinical (diagnostic) setting, detailed analysis of the lectin, including cloning and recombinant ... More
Heterogeneous nuclear ribonucleoprotein (hnRNP) E1 binds to hnRNP A2 and inhibits translation of A2 response element mRNAs.
Authors:Kosturko LD, Maggipinto MJ, Korza G, Lee JW, Carson JH, Barbarese E
Journal:Mol Biol Cell
PubMed ID:16775011
'Heterogeneous nuclear ribonucleoprotein (hnRNP) A2 is a trans-acting RNA-binding protein that mediates trafficking of RNAs containing the cis-acting A2 response element (A2RE). Previous work has shown that A2RE RNAs are transported to myelin in oligodendrocytes and to dendrites in neurons. hnRNP E1 is an RNA-binding protein that regulates translation of ... More
Photolithographic patterning of polyethylene glycol hydrogels.
Authors:Hahn MS, Taite LJ, Moon JJ, Rowland MC, Ruffino KA, West JL
Journal:Biomaterials
PubMed ID:16375965
'A simple, inexpensive photolithographic method for surface patterning deformable, solvated substrates is demonstrated using photoactive poly(ethylene glycol) (PEG)-diacrylate hydrogels as model substrates. Photolithographic masks were prepared by printing the desired patterns onto transparencies using a laser jet printer. Precursor solutions containing monoacryloyl-PEG-peptide and photoinitiator were layered onto hydrogel surfaces. The ... More
Physical interactions of the peroxisomal targeting signal 1 receptor pex5p, studied by fluorescence correlation spectroscopy.
Authors:Wang D, Visser NV, Veenhuis M, van der Klei IJ
Journal:J Biol Chem
PubMed ID:12930827
'We have studied Hansenula polymorpha Pex5p and Pex8p using fluorescence correlation spectroscopy (FCS). Pex5p is the Peroxisomal Targeting Signal 1 (PTS1) receptor and Pex8p is an intraperoxisomal protein. Both proteins are essential for PTS1 protein import and have been shown to physically interact. We used FCS to analyze the molecular ... More
Pathway of actin filament branch formation by Arp2/3 complex revealed by single-molecule imaging.
Authors:Smith BA, Daugherty-Clarke K, Goode BL, Gelles J,
Journal:Proc Natl Acad Sci U S A
PubMed ID:23292935
'Actin filament nucleation by actin-related protein (Arp) 2/3 complex is a critical process in cell motility and endocytosis, yet key aspects of its mechanism are unknown due to a lack of real-time observations of Arp2/3 complex through the nucleation process. Triggered by the verprolin homology, central, and acidic (VCA) region ... More
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