VetMAX™ MastiType Micro4 Kit
VetMAX™ MastiType Micro4 Kit
Applied Biosystems™

VetMAX™ MastiType Micro4 Kit

The VetMAX MastiType Micro4 Kit enables rapid, accurate detection of the four major contagious mastitis-causing pathogens in bovine milk usingRead more
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Catalog NumberQuantity
A39235100 tests
Catalog number A39235
Price (TWD)
-
Quantity:
100 tests
The VetMAX MastiType Micro4 Kit enables rapid, accurate detection of the four major contagious mastitis-causing pathogens in bovine milk using real-time PCR amplification of DNA unique to each pathogen in a single reaction.

The advanced technology of VetMAX MastiType real-time PCR assays offers several advantages:
• Designed for laboratories requiring high-throughput processing methods
• Faster results—typical time from sample to result is 2.5–3 hours, compared to up to 10 days for a culture
• High accuracy—high sensitivity and specificity for all individual pathogens
• Identifies and quantifies DNA, allowing detection of viable, dead, and growth-inhibited pathogens
• Allows use of preserved milk samples, eliminating the need for cooling during sample transportation
• Can be integrated into milk recording programs using preserved milk
• Validated with convenient MagMAX CORE nucleic acid purification method that does not require aspiration and centrifugation

The VetMAX MastiType Micro4 assay detects these four pathogens in a single PCR reaction:
Staphylococcus aureus
Streptococcus agalactiae
Mycoplasma bovis
Streptococcus uberis

Specifications
For Use With (Equipment)7500 Fast System, 7500 System, QuantStudio™
No. of Reactions1 Reaction
Product LineVetMAX
Product TypePCR Reagents and Detection Kit for Animal Health
Quantity100 tests
Sample TypeDNA
Shipping ConditionDry Ice
Detection MethodPrimer-probe
GC-Rich PCR PerformanceHigh
PCR MethodMultiplex qPCR
Reaction SpeedFast or Standard
Unit SizeEach
Contents & Storage
• 1 x 1.2 mL MastiType Master Mix. Contains a Hot-Start DNA polymerase in an optimized PCR buffer with magnesium and dNTPs.
• 1 x 440 μL MastiType Positive Control. A positive control for the PCR reaction components.
• 1 x 550 μL MastiType Micro4 Primer Mix. Includes primers for multiplex PCR amplification of S. aureus, Str. agalactiae, M. bovis, and S. uberis, and an Internal Amplification Control (IAC).

Store at -5 to -30°C.

Frequently asked questions (FAQs)

What kind of milk samples can be tested with the VetMAX MastiType kits?

All VetMAX MastiType kits may be used with milk, bulk milk, frozen milk, or preserved milk (e.g., with bronopol).

What qPCR instrument can I use to run the VetMAX MastiType kits?

Either the Applied Biosystems 7500/7500 Fast Real-Time PCR System or the QuantStudio 5 Real-Time PCR System may be used to run the VetMAX MastiType kits.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Which sample prep kit do you recommend upstream of the VetMAX MastiType kits?

We recommend using the MagMAX CORE Nucleic Acid Purification Kit + MagMAX CORE Mastitis & Panbacteria Module (Cat. No. A40289) with the VetMAX MastiType kits.

Which organisms can be detected by each VetMAX MastiType kit?

The VetMax MastiType Micro4 Kit detects Mycoplasma bovis, Staphylococcus aureus, Streptococcus agalactiae, and Streptococcus uberis.

The VetMax MastiType Myco8 Kit detects Mycoplasma spp., Mycoplasma alkalescens, Mycoplasma bovis, Mycoplasma bovigenitalium, Mycoplasma canadense, Mycoplasma californicum, Staphylococcus aureus, and Streptococcus agalactiae.

The VetMax MastiType Multi Kit detects Staphylococcus aureus, Staphylococcus spp. (including all major coagulase-negative staphylococci), Streptococcus agalactiae, Streptococcus dysgalactiae, Streptococcus uberis, Escherichia coli, Enterococcus spp. (incl. E. faecalis and E. faecium), Klebsiella oxytoca and/or Klebsiella pneumoniae, Serratia marcescens, Corynebacterium bovis, Trueperella pyogenes and/or Peptoniphilus indolicus, Staphylococcal beta-lactamase gene (penicillin-resistance gene), Mycoplasma bovis, Mycoplasma spp., Yeast, and Prototheca spp.