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Citations & References (24)
Invitrogen™
Cascade Blue™ hydrazide, Trisodium Salt
The cell-impermeant, fixable, polar tracer Cascade Blue™ hydrazide can be used to investigate cell-cell and cell-liposome fusion as well asRead more
| Catalog Number | Quantity |
|---|---|
| C687 | 10 mg |
Catalog number C687
Price (TWD)
10,430.00
Online offer
Ends: 31-Dec-2025
14,900.00Save 4,470.00 (30%)
Each
Quantity:
10 mg
Price (TWD)
10,430.00
Online offer
Ends: 31-Dec-2025
14,900.00Save 4,470.00 (30%)
Each
The cell-impermeant, fixable, polar tracer Cascade Blue™ hydrazide can be used to investigate cell-cell and cell-liposome fusion as well as membrane permeability and transport through gap junctions or cell uptake during pinocytosis. This water-soluble tracer can be introduced into cells by whole-cell patch clamping, iontophoresis, osmotic lysis of pinocytic vesicles or comparable methods. Additionally, carboxylic acids of proteins and other water-soluble biopolymers can be coupled to this molecule in aqueous solution using water-soluble carbodiimides such as EDAC (Cat. No. E2247).
To make a concentrated stock solution, dissolve in H2O up to ∼1% (w/v). Store frozen and protected from light.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Chemical ReactivityCarboxylic Acid, Ketone, Aldehyde
Emission412 nm
Excitation399 nm
Label or DyeCascade Blue™
Molecular Weight (g/mol)596.44 G/MOL
Product TypeHydrazide
Quantity10 mg
Reactive MoietyAmine, Hydrazide
Shipping ConditionRoom Temperature
Label TypeClassic Dyes
Product LineCASCADE BLUE
Unit SizeEach
Contents & Storage
Store at room temperature and protect from light.
Frequently asked questions (FAQs)
What is the recommended concentration for preparation of Cascade Blue hydrazide, Trisodium Salt (Cat. No. C687) stock solution?
Citations & References (24)
Citations & References
Abstract
Parathyroid hormone controls the size of the intracellular Ca(2+) stores available to receptors linked to inositol trisphosphate formation.
Journal:J Biol Chem
PubMed ID:10636879
'In HEK 293 cells stably expressing type 1 parathyroid (PTH) receptors, PTH stimulated release of intracellular Ca(2+) stores in only 27% of cells, whereas 96% of cells responded to carbachol. However, in almost all cells PTH potentiated the response to carbachol by about 3-fold. Responses to carbachol did not desensitize,
Neuropeptides exert direct effects on rat thymic epithelial cells in culture.
Journal:Dev Immunol
PubMed ID:9716910
'To determine if major thymic neuropeptides and neurotransmitters can directly influence the functional activity of cultured rat thymic epithelium, neuropeptides and neurotransmitters were applied, and intercellular communication, proliferation, and thymulin secretion assessed. After injections of a mixture of lucifer yellow dextran (too large to pass gap junctions) and cascade blue
Oligosaccharide organization of the beta-subunits of pig kidney Na+/K+-ATPase.
Journal:Physiol Res
PubMed ID:9730046
'The distance between the beta-subunits of Na+/K+-ATPase isolated from pig dark red kidney medulla was determined by Förster energy transfer. First, oligosaccharides of the beta-subunit were shown to be labelled with three fluorophores: Lucifer yellow (LY), Lissamine rhodamine B sulfonyl hydrazine (LRSH) and Cascade blue (CB). Further, LY and LRSH
Site-directed conjugation of nonpeptide groups to peptides and proteins via periodate oxidation of a 2-amino alcohol. Application to modification at N-terminal serine.
Journal:Bioconjug Chem
PubMed ID:1325188
'The 2-amino alcohol structure -CH(NH2)CH(OH)- exists in proteins and peptides in N-terminal Ser or Thr and in hydroxylysine. Its very rapid oxidation by periodate at pH 7 generates an aldehyde in the peptide and is the first step in a method for site-directed labeling with biotin or a fluorescent reporter.
Functional analysis of amino acid sequences in connexin43 involved in intercellular communication through gap junctions.
Journal:J Cell Sci
PubMed ID:7615666
'Gap junctions allow direct communication between cells without recourse to the extracellular space and have been widely implicated as important mediators of cell-cell signalling. They are constructed from the connexin proteins, which form a large family, and individual connexins show complex spatial and temporal variations in their expression patterns. Understanding