DH5α Competent Cells for Subcloning

Thermo Scientific DH5α Competent Cells for Subcloning are chemically competent E. coli cells recommended for routine subcloning into plasmid vectors. Subcloning efficiency cells are not suitable for the generation of cDNA libraries. The φ80dlacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors to allow blue/white colony screening on bacterial agar plates containing Bluo-Gal or X-Gal.

• Transformation efficiency: >1x10⁶ cfu/μg pUC19 DNA
• Suitable for routine cloning applications
• Convenient economical packaging
• Contain genetic markers that allow for blue/white colony screening

Applications
DH5α Competent Cells for Subcloning are suitable for the standard DNA cloning applications:
• DNA cloning following PCR or restriction digestion reactions
• Subcloning (transferring DNA from a parent vector to a destination vector)
• Plasmid isolation

Genotype
F– φ80lacZΔ M15 Δ (lacZYA-argF) U169 recA1 endA1 hsdR17 (rK– mK+) phoA supE44 λ- thi–1 gyrA96 relA1