Free Fatty Acids (NEFA/FFA) Fluorometric Assay Kit

Free fatty acids are converted to acyl coenzyme A in the presence of acyl synthase. This acyl coenzyme A then reacts with acyl oxidase to produce hydrogen peroxide. In the presence of a specific enzyme and probe, hydrogen peroxide reacts to form a fluorescent substrate. The fluorescence intensity, measured at an excitation wavelength of 535 nm and an emission wavelength of 590 nm, is directly proportional to the concentration of free fatty acids in the sample. This kit is useful for researchers studying lipid metabolism, diabetes, obesity, and related fields.

Key features

• Simple and fast protocol—user-friendly, requiring minimal hands-on time and providing rapid results within a short incubation period
• Broad range of kits across key analytes and targets within the most important metabolic pathways
• Consistent—strict quality control criteria to ensure reproducible and reliable results