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Citations & References (107)
Invitrogen™
Fluorescein Di-β-D-Galactopyranoside (FDG)
Fluorescein di-β-D-galactopyranoside (FDG) is one of the most sensitive substrates for galactosidases. Nonfluorescent FDG is sequentially hydrolyzed by β-galactosidase, firstRead more
| Catalog Number | Quantity |
|---|---|
| F1179 | 5 mg |
Catalog number F1179
Price (TWD)
11,550.00
Online offer
Ends: 31-Dec-2025
16,500.00Save 4,950.00 (30%)
Each
Quantity:
5 mg
Price (TWD)
11,550.00
Online offer
Ends: 31-Dec-2025
16,500.00Save 4,950.00 (30%)
Each
Fluorescein di-β-D-galactopyranoside (FDG) is one of the most sensitive substrates for galactosidases. Nonfluorescent FDG is sequentially hydrolyzed by β-galactosidase, first to fluorescein monogalactoside (FMG) and then to highly fluorescent fluorescein. Enzyme-mediated hydorlysis of FDG can be followed by the increase in either absorbance or fluorescence.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell PermeabilityCell-permeant
Excitation/Emission490/514
For Use With (Equipment)Fluorescence Microscope, Fluorometer, Flow Cytometer
Quantity5 mg
Shipping ConditionRoom Temperature
SubstrateBeta-Gal Substrate
Detection MethodFluorescence
Substrate PropertiesChemical Substrate
Unit SizeEach
Contents & Storage
Store in freezer (-5 to -30°C).
Citations & References (107)
Citations & References
Abstract
Detection of rare antigen-presenting cells by the lacZ T-cell activation assay suggests an expression cloning strategy for T-cell antigens.
Journal:Proc Natl Acad Sci U S A
PubMed ID:1378619
'The alpha/beta T-cell receptor a complex ligand formed by the association of antigenic peptides with molecules of the major histocompatibility complex (MHC). The inherent limitations of the conventional T-cell activation assays used to detect these peptide/MHC ligands have, until now, hampered the development of expression cloning systems for T-cell antigens.
Primitive adult hematopoietic stem cells can function as osteoblast precursors.
Journal:Proc Natl Acad Sci U S A
PubMed ID:14673088
'Osteoblasts are continually recruited from stem cell pools to maintain bone. Although their immediate precursor is a plastic-adherent mesenchymal stem cell able to generate tissues other than bone, increasing evidence suggests the existence of a more primitive cell that can differentiate to both hematopoietic and mesenchymal cells. We show here
Retroviral gene transfer is inhibited by chondroitin sulfate proteoglycans/glycosaminoglycans in malignant pleural effusions.
Journal:J Biol Chem
PubMed ID:9115227
'Gene therapy may be an important adjuvant for treating cancer in the pleural space. The initial results of retroviral gene transfer to cancer cells in malignant pleural effusions revealed that transduction was markedly inhibited, and studies to characterize the inhibitory factor(s) were performed. The inhibition was contained within the soluble,
In situ detection of transcriptionally active chromatin and genetic regulatory elements in individual viable mammalian cells.
Journal:Immunol Suppl
PubMed ID:2807403
'Using a newly developed FACS method for quantifying the expression of the Escherischia coli lacZ reporter gene in viable mammalian cells, we have obtained cloned cell lines in which the expression of lacZ is under the control of native endogenous transcription elements. We infected the murine pre-B cell 70Z/3 with
Transcriptional activity of the neuron-specific enolase (NSE) promoter in murine embryonic stem (ES) cells and preimplantation embryos.
Journal:Eur J Cell Biol
PubMed ID:7925488
'Mouse embryonic stem (ES) cells were transfected with a plasmid composed of an E. coli lacZ gene fused to 1.8 kb of rat neuron-specific enolase (NSE) promoter sequences. While this reporter construct had been shown previously to function exclusively in postmitotic neurons and neuro-endocrine cells of transgenic mice, stably transfected